本文選題：持續(xù)運(yùn)動 + 間歇運(yùn)動��； 參考：《云南師范大學(xué)》2017年碩士論文

【摘要】：研究目的:運(yùn)動科學(xué)領(lǐng)域越來越多的研究表明,適宜的運(yùn)動對學(xué)習(xí)記憶能力具有促進(jìn)作用。本研究通過6周有氧跑臺訓(xùn)練對SD大鼠學(xué)習(xí)記憶能力、海馬和皮質(zhì)氧化抗氧化的影響,探討持續(xù)與間歇運(yùn)動促進(jìn)大鼠學(xué)習(xí)記憶的提高,其作用機(jī)制可能是通過改變MDA、SOD代謝及NO的表達(dá)來實(shí)現(xiàn)。研究方法:實(shí)驗(yàn)動物進(jìn)行適應(yīng)性飼養(yǎng)后進(jìn)行篩選,篩選方法采用水迷宮可視平臺實(shí)驗(yàn),淘汰疑似先天癡呆的大鼠。選取健康雄性成年SD大鼠24只,隨機(jī)分為空白對照組(n=8)、持續(xù)訓(xùn)練組(n=8)和間歇訓(xùn)練組(n=8)。在正式實(shí)驗(yàn)前進(jìn)行1周的適應(yīng)性跑臺訓(xùn)練。跑臺正式訓(xùn)練采用遞增負(fù)荷形式,起始速度為22m/min,坡度為0,每周遞增1m/min,進(jìn)行6周,最后一周速度為27m/min;持續(xù)組運(yùn)動40min,間歇組每次運(yùn)動20min,每天2次,兩次運(yùn)動間休息3小時。跑臺訓(xùn)練結(jié)束后三組大鼠進(jìn)行為期5天的Morris水迷宮實(shí)驗(yàn)(4天定位航行實(shí)驗(yàn),1天空間探索實(shí)驗(yàn))。在水迷宮測試后禁食禁水24h后,用3.6%水合氯醛腹腔注射麻醉,取海馬、皮質(zhì)。使用試劑盒測試大鼠海馬、皮質(zhì)MDA含量、SOD活力、NO含量。研究結(jié)果:(1)Morris水迷宮檢測結(jié)果:定位航行實(shí)驗(yàn)結(jié)果顯示,縱向比較發(fā)現(xiàn),定位航行實(shí)驗(yàn)中,隨訓(xùn)練天數(shù)的增加,各組潛伏期減小,站臺象限路程%和時間%增加。橫向比較發(fā)現(xiàn),第2天,運(yùn)動組潛伏期與對照組有顯著性差異(p0.05);第3天,與對照組相比,持續(xù)組(p0.05)與間歇組(p0.01)下降明顯,其中間歇組與持續(xù)組相比,間歇組明顯下降(p0.05);第4天,運(yùn)動組潛伏期顯著低于對照組(p0.01)。站臺象限路程%在4天的測試中都是間歇組最高;第3天間歇組顯著高于對照組和持續(xù)組(p0.05),第4天間歇組明顯高于對照組(p0.05)。在4天的測試中,站臺象限時間%間歇組最高,持續(xù)組次之,對照組最低;第3天間歇組站臺象限時間%高于對照組,差異極具顯著性(p0.01);第4天間歇組明顯高于持續(xù)組和對照組,差異極具顯著性(p0.01)。空間探索實(shí)驗(yàn)結(jié)果顯示,與對照組相比,持續(xù)組與間歇組穿臺次數(shù)顯著增加(p0.01),間歇組多于持續(xù)組,但無差異;持續(xù)組與間歇組到達(dá)潛伏期顯著低于對照組(p0.01),間歇組低于持續(xù)組,運(yùn)動組間無差異;站臺象限路程%與站臺象限時間%間歇組顯著高于對照組(p0.05),兩運(yùn)動組間無差異;運(yùn)動組總路程顯著高于對照組(p0.01),運(yùn)動組間無差異;平均速度持續(xù)組(p0.05)與間歇組(p0.01)都顯著高于對照組,但運(yùn)動組間無差異。(2)海馬氧化抗氧化能力結(jié)果:與對照組相比,持續(xù)組和間歇組MDA含量顯著降低(P0.01),間歇組低于持續(xù)組,但無差異;持續(xù)組與間歇組SOD活性顯著高于對照組(P0.01),間歇組高于持續(xù)組,但組間無差異。與對照組比較,運(yùn)動組NO含量較高,其中,間歇組顯著高于對照組(P0.05),但持續(xù)組和間歇組無差異。(3)皮質(zhì)氧化抗氧化能力結(jié)果:持續(xù)組、間歇組MDA含量低于對照組(P0.05),間歇組低于持續(xù)組,但組間無差異;持續(xù)組與間歇組SOD活性高于對照組,間歇組高于持續(xù)組,但組間無差異;NO檢測結(jié)果顯示,間歇組最高,持續(xù)組次之,對照組最低,三組大鼠之間無差異(P0.05)。研究結(jié)論:六周有氧跑臺運(yùn)動改善了大鼠的空間學(xué)習(xí)記憶能力,使大鼠大腦海馬和皮質(zhì)的SOD、NO活性增高,MDA含量下降,減弱了脂質(zhì)過氧化對海馬和皮質(zhì)的損傷,間歇運(yùn)動與持續(xù)運(yùn)動相比,間歇運(yùn)動對增強(qiáng)學(xué)習(xí)記憶的效果以及清除自由基的能力強(qiáng)于持續(xù)運(yùn)動。六周跑臺運(yùn)動促進(jìn)學(xué)習(xí)記憶的提高可能與運(yùn)動提高海馬、皮質(zhì)對自由基損傷的抵抗能力,使機(jī)體對自由基產(chǎn)生耐受力和適應(yīng)力,從而提高抗氧化能力以及提高NO的表達(dá)有關(guān)。
[Abstract]:Research purposes: more and more studies in the field of sports science have shown that appropriate exercise has a promoting effect on learning and memory ability. In this study, the effects of 6 week aerobic table training on the learning and memory ability of SD rats, the oxidation and oxidation resistance of hippocampus and cortex, and the improvement of learning and memory in rats by continuous and intermittent movement were discussed. It may be realized by changing the expression of MDA, SOD metabolism and NO. Research methods: the experimental animals were screened by adaptive feeding, and the screening methods were selected by the water maze visual platform experiment, and the rats with suspected congenital dementia were eliminated. 24 healthy adult male SD rats were selected and randomly divided into blank control group (n=8), continuous training group (n=8) and room. The training group (n=8) was trained for 1 weeks prior to the formal experiment. The formal training used the incremental load form, the starting speed was 22m/min, the gradient was 0, the weekly increase of 1m/min, the 6 week, the last week was 27m/min, the continuous group movement 40min, the interval group each exercise 20min, the two times between the 3 hours between the two exercises. After the end of the training, the three groups of rats were conducted a 5 day Morris water maze test (4 days positioning navigation experiment, 1 day space exploration experiment). After the water maze test was forbidden to water 24h, the hippocampus and cortex were taken with 3.6% chloral chloral injection, the hippocampus and the cortex were taken to test the rat sea horse, the cortical MDA content, the SOD activity, and the NO content. The results were (1) M The results of orris water maze test: the results of the navigation experiment showed that the longitudinal comparison found that the incubation period decreased with the increase of the number of training days in the navigation experiment, and the horizontal comparison found that the incubation period of the sports group was significantly different from the control group (P0.05) on the second day, and the third day, compared with the control group. Group (P0.05) and intermittent group (P0.01) decreased significantly, among which intermittent group was significantly lower than that in continuous group (P0.05); on the fourth day, the latency of the exercise group was significantly lower than that of the control group (P0.01). The platform quadrant route% was the highest in the intermittent group in the 4 day test; the third day interval group was significantly higher than the control group and the continuous group (P0.05), and the fourth day intermittent group was obvious. Higher than the control group (P0.05). In the 4 day test, the platform quadrant time interval group was the highest, the continuous group, the control group was the lowest, the third day interval group platform quadrant time% was higher than the control group, the difference was significant (P0.01); the fourth day interval group was significantly higher than the continuous group and the group, the difference was very significant (P0.01). Spatial exploration experimental results showed that the results showed that Compared with the control group, the number of times in the continuous group and the intermittent group increased significantly (P0.01), and the intermittent group was more than the continuous group, but there was no difference. The duration of the interval group and the intermittent group was significantly lower than that of the control group (P0.01). The intermittent group was lower than the continuous group, and there was no difference between the group and the exercise group; the station platform quadrant% and the platform quadrant time interval group were significantly higher than the control group (p0.0 5) there was no difference between the two exercise groups, the total distance of the exercise group was significantly higher than that of the control group (P0.01), and there was no difference between the exercise groups, and the average speed continuous group (P0.05) and the intermittent group (P0.01) were significantly higher than the control group, but there was no difference between the exercise groups. (2) the oxidation antioxidant capacity of the hippocampus: compared with the control group, the MDA content in the continuous group and the intermittent group was significantly lower (P0.01). The interval group was lower than the continuous group, but no difference was found in the continuous group and the intermittent group SOD activity was significantly higher than the control group (P0.01), the intermittent group was higher than the continuous group, but there was no difference between the group and the control group. Compared with the control group, the NO content of the exercise group was higher than that of the control group (P0.05), but there was no difference between the continuous group and the intermittent group. (3) the oxidation antioxidant capacity of the cortex was found. Fruit: in the continuous group, the MDA content in the intermittent group was lower than the control group (P0.05), the intermittent group was lower than the continuous group, but there was no difference between the group and the group. The SOD activity in the continuous group and the intermittent group was higher than the control group. The intermittent group was higher than the continuous group, but there was no difference between the groups. The results of NO test showed that the interval group was the highest, the continued group was the lowest, and the three groups of rats had no difference (P0.05). Conclusion: the six week aerobic table exercise improved the spatial learning and memory ability of rats, increased the activity of SOD and NO in the hippocampus and cortex of the rat brain, decreased the content of MDA, and weakened the damage to the hippocampus and cortex by lipid peroxidation. Intermittent movement could enhance the effect of learning and memory and scavenge free radicals, compared with the continuous movement. The improvement of learning and memory in the six week treadmill exercise may improve the resistance of the hippocampus, the cortex to the free radical damage, the tolerance and adaptability of the body to the free radicals, so as to improve the antioxidant capacity and to improve the expression of NO.

【學(xué)位授予單位】：云南師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：G804.2

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陶家祥;大鼠基因組成功破譯的重大科學(xué)價值[J];世界科學(xué);2004年05期

2 陳偉強(qiáng);趙善廣;;自制注射用大鼠固定裝置[J];上海實(shí)驗(yàn)動物科學(xué);1992年04期

3 馬蘭軍;毛雁;熊正英;;茶多酚補(bǔ)充對游泳大鼠脂肪代謝的影響及機(jī)理研究[J];武漢體育學(xué)院學(xué)報;2008年03期

4 胡敏;鄒亮疇;劉承宜;任飛;;大鼠抗阻訓(xùn)練模型的歸類與分析[J];廣州體育學(xué)院學(xué)報;2009年05期

5 崔玉鵬;張凡;王保成;宋玉濤;楊衛(wèi)平;;大負(fù)荷游泳訓(xùn)練與模擬失重對大鼠下丘腦單胺類神經(jīng)遞質(zhì)影響的對比研究[J];武漢體育學(xué)院學(xué)報;2009年06期

6 羅崇彬;藍(lán)金全;;清芝靈對高脂血癥模型大鼠血脂的調(diào)控作用[J];宜春學(xué)院學(xué)報(自然科學(xué));2006年02期

7 王成績;;游泳對載脂蛋白E敲除大鼠的脂代謝和動脈粥樣硬化形成的影響[J];宜春學(xué)院學(xué)報;2011年12期

8 郭慶;譚德勇;;藥物處理心肌缺血大鼠有效性的研究[J];昆明師范高等�？茖W(xué)校學(xué)報;2005年04期

9 茹今;;貓與老鼠[J];雜文選刊(下旬版);2010年08期

10 張洪海;王人衛(wèi);黃雅君;檀志宗;;遞增負(fù)荷運(yùn)動對大鼠腎上腺、甲狀腺軸激素的影響[J];北京體育大學(xué)學(xué)報;2008年01期

相關(guān)會議論文 前10條

1 尹音;孫振宇;胡敏;李冬霞;;持續(xù)性高正加速度對大鼠顳頜關(guān)節(jié)損傷的作用[A];第八屆全國顳下頜關(guān)節(jié)病學(xué)及（牙合）學(xué)大會論文匯編[C];2011年

2 祝~=驤;iJ梊霞;洃克琴;崔素英;文允摪;，

本文編號：1841819