本文選題：運動 + 慢性心力衰竭　； 參考：《中國老年學雜志》2017年08期

【摘要】：目的觀察8 w運動對心力衰竭大鼠左室結(jié)構(gòu)、功能和重構(gòu)基因表達的影響,探討運動改善左室重構(gòu)的可能機制。方法 40只SD大鼠隨機分為心力衰竭對照組(HC組,n=10)、心力衰竭運動組(HE組,n=10)、假手術(shù)對照組(SC組,n=10)和假手術(shù)運動組(SE組,n=10),心力衰竭模型采用冠狀動脈結(jié)扎術(shù)。HE組和SE組進行8 w跑臺運動,SC組和HC組在鼠籠內(nèi)自由活動。超聲心動圖檢測左室結(jié)構(gòu)和功能;分離左室后行Masson染色法進行組織病理學觀察并獲得膠原容積分數(shù)(CVF);實時熒光定量PCR檢測左室心肌心鈉素(ANF)、α-肌球蛋白重鏈(α-MHC)、β-肌球蛋白重鏈(β-MHC)和肌質(zhì)網(wǎng)Ca~(2+)-ATP酶(SERCA2a)mRNA表達量。結(jié)果 (1)與SC組比較,SE組大鼠左室出現(xiàn)離心性肥大,心功能提高,α-MHC和SERCA2a mRNA升高(P0.05);(2)與SC組比較,HC組左室出現(xiàn)向心性肥大,心功能降低,CVF明顯增加(P0.05),心肌α-MHC和SERCA2a mRNA表達下調(diào)(P0.05),ANF和β-MHC mRNA表達上調(diào)(P0.05);(3)與HC組比較,HE組大鼠左室發(fā)生離心性肥大,心功能提高,CVF明顯減少(P0.05),心肌α-MHC和SERCA2a mRNA表達上調(diào)(P0.05),ANF和β-MHC mRNA表達下調(diào)(P0.05)。結(jié)論長期運動抑制心力衰竭大鼠左室重構(gòu)并改善心功能,其機制與心肌纖維化程度減輕、胚胎基因表達下調(diào)、收縮蛋白表達上調(diào)有關(guān)。
[Abstract]:Objective to observe the effects of exercise for 8 weeks on left ventricular structure, function and remodeling gene expression in rats with heart failure, and to explore the possible mechanism of improving left ventricular remodeling by exercise.Methods Forty Sprague-Dawley rats were randomly divided into three groups: heart failure control group (HC group), heart failure exercise group (HE group), sham operation control group (SC group) and sham operation exercise group (SE group). Coronary artery ligation was performed on heart failure model.Group C and group HC moved freely in cage for 8 weeks.Left ventricular structure and function were measured by echocardiography.Masson staining was used to observe the histopathology of left ventricle and collagen volume fraction was obtained. The expression of left ventricular atrial natriuretic peptide (ANF), 偽 -myosin heavy chain (尾 -MHCC), 尾 -myosin heavy chain (尾 -MHC1) and sarcoplasmic reticulum (Ca~(2) -ATPase SERCA2a mRNA was detected by real-time quantitative PCR.Results compared with SC group, left ventricular eccentricity was found in SE group, cardiac function was increased, 偽 -MHC and SERCA2a mRNA were increased (P 0.05) compared with SC group, left ventricular hypertrophy appeared in HC group.The expression of 偽 -MHC and SERCA2a mRNA was down-regulated and the expression of P0.05 and 尾 -MHC mRNA was upregulated (P0.05). Compared with the HC group, the left ventricular eccentricity occurred in the HE group.The expression of 偽 -MHC and SERCA2a mRNA was up-regulated and the expression of P0.05 and 尾 -MHC mRNA was down-regulated.Conclusion Long-term exercise inhibits left ventricular remodeling and improves cardiac function in rats with heart failure. The mechanism is related to the decrease of myocardial fibrosis, down-regulation of embryonic gene expression and up-regulation of contractile protein expression.
【作者單位】： 鄭州大學體育系;
【基金】：河南省體育局項目(HT2013-05)
【分類號】：G804.7
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本文編號：1767418