本文選題：骨骼肌細(xì)胞　切入點(diǎn)：不同負(fù)荷運(yùn)動　出處：《動物醫(yī)學(xué)進(jìn)展》2017年06期 　論文類型：期刊論文

【摘要】：探討不同負(fù)荷運(yùn)動對小鼠骨骼肌細(xì)胞凋亡及mTOR、Akt蛋白表達(dá)的影響。30只小鼠分為3組,依次為對照組(C)、大強(qiáng)度運(yùn)動組(H)、力竭組(G)。對照組不做運(yùn)動訓(xùn)練。大強(qiáng)度運(yùn)動組(H)給予25m/min的運(yùn)動訓(xùn)練。力竭組(G)給予10 m/min的運(yùn)動訓(xùn)練至力竭。6周運(yùn)動訓(xùn)練結(jié)束后,取腓腸肌,TUNEL檢測細(xì)胞凋亡情況。Western blot檢測組織中Bcl-2、Bax、mTOR、Akt蛋白水平。雙縮脲法檢測丙二醛(MDA)含量。黃嘌呤氧化酶法檢測SOD活性。結(jié)果表明,大強(qiáng)度運(yùn)動組(H)和力竭組(G)細(xì)胞凋亡指數(shù)均高于對照組(C),大強(qiáng)度運(yùn)動組(H)比力竭組(G)細(xì)胞凋亡指數(shù)高,大強(qiáng)度運(yùn)動組(H)和力竭組(G)Bax蛋白水平均高于對照組(C);大強(qiáng)度運(yùn)動組(H)Bax水平高于力竭組(G),大強(qiáng)度運(yùn)動組(H)和力竭組(G)Bcl-2、mTOR、p-Akt蛋白水平均低于對照組(C),大強(qiáng)度運(yùn)動組(H)Bcl-2、mTOR、p-Akt蛋白水平低于力竭組(G);大強(qiáng)度運(yùn)動組(H)和力竭組(G)Akt蛋白水平與對照組(C)相比沒有明顯變化;大強(qiáng)度運(yùn)動組(H)和力竭組(G)MDA含量均高于對照組(C),大強(qiáng)度運(yùn)動組(H)MDA含量高于力竭組(G);大強(qiáng)度運(yùn)動組(H)和力竭組(G)SOD活性均低于對照組(C),大強(qiáng)度運(yùn)動組(H)SOD活性高于力竭組(G);大強(qiáng)度運(yùn)動和力竭運(yùn)動均會引起骨骼肌細(xì)胞的凋亡,且大強(qiáng)度運(yùn)動比力竭運(yùn)動更易引起骨骼肌細(xì)胞凋亡。
[Abstract]:To investigate the effect of different load exercise on the apoptosis of skeletal muscle cells and the expression of mTORA Akt protein in mice, 30 mice were divided into three groups. The order was: control group, high intensity exercise group, exhaustion group, no exercise training. High intensity exercise group was given 25 m / min exercise training. Exhaustion group was given 10 m / min exercise training until the end of exhaustive 6 weeks exercise training, and the control group was given 25 m / min exercise training, and the exhaustion group was given 10 m / min exercise training until the end of exhaustive exercise training at the end of week 6. Tunel from gastrocnemius muscle was used to detect apoptosis. Western blot was used to detect the protein level of Bcl-2Bax-mTORA. Biuret method was used to detect the content of malondialdehyde (MDA). Xanthine oxidase method was used to detect the activity of SOD. The apoptotic index of high intensity exercise group and exhaustive group were higher than that of control group, and the apoptosis index of high intensity exercise group was higher than that of exhausted group. High intensity exercise group and exhaustive group were higher than the control group, the high intensity exercise group was higher than the exhausted group, the high intensity exercise group was higher than the exhausted group, and the high intensity exercise group had lower protein levels than the control group, and the high intensity exercise group had lower HBcl-2mTORP -Akt protein than the control group, and the high intensity exercise group had lower HBcl-2mTORp-Akt protein level than that of the control group, while the high intensity exercise group was higher than that of the exhausted group, and the high intensity exercise group was higher than the exhausted group, and the high intensity exercise group had lower protein levels than the control group. Compared with the control group, the levels of G _ (+) -Akt protein in the high intensity exercise group and the exhausted group were not significantly changed, while those in the exhausted group were lower than those in the control group (P < 0.05), but there was no significant difference between the two groups. The content of MDA in the high intensity exercise group and the exhausted group was higher than that in the control group, and the content of MDA in the high intensity exercise group was higher than that in the exhausted group, while the activity of GG SOD in the high intensity exercise group and the exhausted group was lower than that in the control group, while in the high intensity exercise group it was higher than that in the exhaustive exercise group. High intensity exercise and exhaustive exercise could induce apoptosis of skeletal muscle cells. High intensity exercise is more likely to induce skeletal muscle cell apoptosis than exhaustive exercise.
【作者單位】： 廣西大學(xué)體育學(xué)院;廣西醫(yī)科大學(xué)第一附屬醫(yī)院;廣西醫(yī)科大學(xué)附屬口腔醫(yī)院;
【分類號】：G804.2;R87

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