發(fā)布時(shí)間：2018-02-22 17:10

  本文關(guān)鍵詞： 腦缺血 微透析 紋狀體 多巴胺 被動(dòng)回避反射　出處：《首都體育學(xué)院》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：研究目的:實(shí)驗(yàn)和臨床研究已經(jīng)證明,運(yùn)動(dòng)能對(duì)腦缺血產(chǎn)生神經(jīng)保護(hù)的作用,但是此過(guò)程中的潛在神經(jīng)化學(xué)機(jī)制還有待進(jìn)一步研究。本研究的目的是通過(guò)探究主動(dòng)跑輪運(yùn)動(dòng)促進(jìn)腦缺血后認(rèn)知功能恢復(fù)的紋狀體腦區(qū)多巴胺的動(dòng)態(tài)變化,由此探討主動(dòng)跑輪運(yùn)動(dòng)改善腦缺血損傷的機(jī)制。研究方法:選取健康3月齡雄性SD大鼠24只,隨機(jī)將其分為4組:運(yùn)動(dòng)4周缺血組(4RI組)、運(yùn)動(dòng)1周缺血組(1RI組)、缺血對(duì)照組(I組)、假手術(shù)組(C組),每組6只。運(yùn)動(dòng)4周缺血組、運(yùn)動(dòng)1周缺血組大鼠分別進(jìn)行為期4周和1周的主動(dòng)跑輪訓(xùn)練,缺血對(duì)照組和假手術(shù)組不進(jìn)行主動(dòng)跑輪訓(xùn)練。在完成4周的主動(dòng)跑輪訓(xùn)練之后,運(yùn)動(dòng)4周缺血組、運(yùn)動(dòng)1周缺血組、缺血對(duì)照組大鼠進(jìn)行永久性雙側(cè)頸總動(dòng)脈結(jié)扎(2-V0)手術(shù),制備大鼠全腦缺血模型,而假手術(shù)組僅暴露雙側(cè)頸總動(dòng)脈,不進(jìn)行結(jié)扎處理。腦缺血前、后運(yùn)用活體腦內(nèi)微透析及高效液相色譜電化學(xué)聯(lián)用技術(shù)對(duì)大鼠紋狀體透析液中的多巴胺水平進(jìn)行測(cè)定,微透析實(shí)驗(yàn)完成后,將所有大鼠灌流取腦并做組織學(xué)鑒定。另外重新選取24只健康3月齡雄性SD大鼠,每組6只,分組及訓(xùn)練方法同上,訓(xùn)練結(jié)束后,對(duì)運(yùn)動(dòng)4周缺血組、運(yùn)動(dòng)1周缺血組、缺血對(duì)照組大鼠進(jìn)行永久性雙側(cè)頸總動(dòng)脈結(jié)扎,制備全腦缺血模型,在腦缺血后24小時(shí)應(yīng)用被動(dòng)回避反射評(píng)價(jià)四組大鼠的學(xué)習(xí)記憶能力。研究結(jié)果:1、與假手組恒定的多巴胺含量相比,I組大鼠2-VO缺血手術(shù)后紋狀體腦區(qū)多巴胺含量顯著上升(P0.001),在缺血手術(shù)后20分鐘上升至最大值,隨后逐漸下降。而4RI組和1RI組大鼠2-VO缺血手術(shù)前后,大鼠紋狀體腦區(qū)多巴胺含量變化無(wú)顯著差異(P0.05)。2、4RI組和1RI組大鼠缺血前紋狀體腦區(qū)多巴胺基礎(chǔ)含量顯著高于C組和I組(P0.001)。但是4RI組和1RI組大鼠缺血前紋狀體腦區(qū)多巴胺基礎(chǔ)含量比較無(wú)顯著差異(P0.05)。3、被動(dòng)回避反射結(jié)果顯示,C組大鼠、4RI組大鼠和1RI組大鼠重復(fù)訓(xùn)練時(shí)間顯著長(zhǎng)于I組大鼠(P0.001)。C組大鼠、4RI組大鼠和1RI組大鼠重復(fù)訓(xùn)練時(shí)間相比無(wú)顯著差異(P0.05)。4、形態(tài)學(xué)研究結(jié)果顯示,微透析探針被準(zhǔn)確放置在大鼠右側(cè)紋狀體,提示腦缺血前后收集的透析液為紋狀體腦區(qū)神經(jīng)細(xì)胞的外液。研究結(jié)論:4周和1周的主動(dòng)跑輪運(yùn)動(dòng)可以顯著提高大鼠紋狀體腦區(qū)多巴胺基礎(chǔ)濃度,并且能抑制大鼠腦缺血后紋狀體腦區(qū)多巴胺的過(guò)度釋放,降低腦缺血對(duì)大鼠學(xué)習(xí)記憶能力的損害。因此,缺血前的主動(dòng)跑輪運(yùn)動(dòng)抑制腦缺血大鼠紋狀體腦區(qū)多巴胺水平的快速上升,可能是運(yùn)動(dòng)促進(jìn)腦缺血大鼠認(rèn)知功能恢復(fù)的潛在神經(jīng)化學(xué)機(jī)制。
[Abstract]:Objective: experimental and clinical studies have shown that exercise has a neuroprotective effect on cerebral ischemia. However, the underlying neurochemical mechanism of this process remains to be further studied. The aim of this study was to explore the dynamic changes of dopamine in the striatum after cerebral ischemia by exploring the effects of active wheel movement on the recovery of cognitive function in the striatum. Methods: 24 healthy male SD rats aged 3 months were selected to study the mechanism of active wheel running in improving cerebral ischemia injury. The rats were randomly divided into 4 groups: 4 weeks ischemia group, 4 weeks ischemia group, 1 week ischemia group, 1 RI group, ischemia control group, sham operation group C group, 6 rats in each group. The rats in the ischemic group underwent 4 weeks and 1 week of active wheel training, while the ischemic control group and the sham operation group did not. One week of exercise ischemia group, ischemic control group rats underwent permanent bilateral common carotid artery ligation (2-V0) operation, the rat model of global cerebral ischemia was made, while the sham operation group only exposed bilateral common carotid artery without ligation. The dopamine levels in rat striatum dialysate were determined by microdialysis in vivo and electrochemical high performance liquid chromatography (HPLC). In addition, 24 healthy male SD rats aged 3 months were re-selected, 6 rats in each group, divided into groups and training methods. After training, 4 weeks ischemia group and 1 week ischemia group were selected. The model of global cerebral ischemia was established by permanent bilateral common carotid artery ligation in rats of ischemic control group. The learning and memory abilities of the four groups were evaluated by passive avoidance reflex 24 hours after cerebral ischemia. Results: 1: 1, compared with the constant dopamine content in the prosthetic hand group, the dopamine content in the striatum of rats in group I was significantly higher than that in group I after 2-VO ischemia. The peak value of P0.001 increased to a maximum at 20 minutes after ischemic surgery. Then decreased gradually. However, before and after 2-VO ischemia in 4RI group and 1RI group, There was no significant difference in dopamine content in the striatum of rats. The dopamine basal levels in the preischemic striatum of the rats in P0.05 and 1RI groups were significantly higher than those in the C and I groups, but the dopa levels in the preischemic striatum of the 4RI and 1RI groups were significantly higher than those of the rats in the preischemic striatum. The results of passive avoidance reflex showed that the time of repeated training in group C and group 1RI was significantly longer than that in group I (P 0.001), group C (n = 4) and group 1RI (n = 1). There was no significant difference between the two groups (P0.05. 4). The morphological results showed that, The microdialysis probe was placed exactly in the right striatum of the rat. The results suggest that the dialysate collected before and after cerebral ischemia is the extracorporeal fluid of the neurons in the striatum. Conclusion the active wheel movement at 4 weeks and 1 week can significantly increase the basic concentration of dopamine in the striatum of rats. And it can inhibit the excessive release of dopamine in striatum after cerebral ischemia, and decrease the impairment of learning and memory ability after cerebral ischemia in rats. The active wheel running before ischemia inhibits the rapid increase of dopamine level in the striatum of rats with cerebral ischemia, which may be a potential neurochemical mechanism by which exercise can promote the recovery of cognitive function in rats with cerebral ischemia.
【學(xué)位授予單位】：首都體育學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：G804.2

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