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抗-P1對輸血相容性檢測的影響

發(fā)布時(shí)間:2019-06-25 08:31
【摘要】:目的回顧性分析8例抗-P1標(biāo)本,為抗-P1引起的輸血相容性檢測困難提供解決方案。方法血型鑒定采用強(qiáng)生Auto VUE玻璃珠卡式法,正反定型不一致樣本用試管法進(jìn)行復(fù)查;抗體篩查用強(qiáng)生Auto VUE抗人球卡進(jìn)行檢測,陽性樣本用Diana抗人球卡做抗體鑒定;試管法鑒定被檢者紅細(xì)胞上是否存在P1抗原,必要時(shí)檢測反定試劑紅細(xì)胞是否存在P1抗原;統(tǒng)計(jì)8例標(biāo)本配血相合率。結(jié)果 8例抗篩陽性標(biāo)本均導(dǎo)致ABO正反定型不符,抗體鑒定結(jié)果均為抗-P1,配血相合率為14%-67%。結(jié)論抗-P1引起正反定型不一致時(shí),應(yīng)使用P1抗原陰性試劑紅細(xì)胞進(jìn)行血型反定型,以確保結(jié)果的準(zhǔn)確性。
[Abstract]:Objective to analyze 8 cases of anti-P1 specimens retrospectively. to provide a solution for the difficulty of detecting the compatibility of blood transfusion caused by anti-P1. Methods Blood group identification was carried out by Johnson Auto VUE glass bead card method, positive and negative inconsistent samples were reexamined by test tube method, antibody screening was detected by Johnson Auto VUE anti-human ball card, positive samples were identified by Diana anti-human ball card, P1 antigen was detected on red blood cells by test tube method, P1 antigen was detected by reverse reagent red blood cells if necessary, and the matching rate of 8 samples was counted. Results the positive and negative typing of ABO was found in all 8 positive samples. The results of antibody identification were anti-P1, and the rate of matching blood phase was 14% 鈮,

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