天堂国产午夜亚洲专区-少妇人妻综合久久蜜臀-国产成人户外露出视频在线-国产91传媒一区二区三区

噬菌體PF23生物學(xué)特性及其對(duì)小鼠肺炎克雷伯菌全身感染療效觀察

發(fā)布時(shí)間:2019-05-29 09:53
【摘要】:目的了解肺炎克雷伯菌裂解性噬菌體PF23的生物學(xué)特性,觀察其對(duì)小鼠全身感染的療效。方法電鏡觀察噬菌體PF23的形態(tài),一步生長(zhǎng)曲線以及對(duì)溫度和pH的敏感性;提取噬菌體基因組,酶切法鑒定其核酸類型,并初步估算其基因組大小;建立小鼠全身感染模型,觀察PF23的治療效果。結(jié)果 PF23的噬菌斑為有暈清晰透明圓斑,直徑約2mm;電鏡觀察PF23具有尾噬菌體目,長(zhǎng)尾病毒科病毒的形態(tài)特征;其在50℃的溫度下及pH 4~9范圍內(nèi)穩(wěn)定性好。一步生長(zhǎng)曲線顯示其潛伏期約為11min,裂解量約為41PFU/細(xì)胞。其基因組核酸不能被雙鏈DNA限制性內(nèi)切酶KpnI、EcoRI和HindIII切開,但能被BamHI切開,且酶片段大小合計(jì)27kb。用PF23治療由其宿主菌所致全身感染小鼠,小鼠的生存期均2d,7d存活率為37.5%;未治療對(duì)照組小鼠1d內(nèi)均死亡。結(jié)論肺炎克雷伯菌的噬菌體PF23潛伏期短,有較好的熱穩(wěn)定性和酸堿穩(wěn)定性,治療小鼠肺炎克雷伯菌全身感染效果顯著,值得進(jìn)一步研究。
[Abstract]:Objective to investigate the biological characteristics of lytic bacteriophage PF23 of Klebsiella pneumoniae and to observe its curative effect on systemic infection in mice. Methods the morphology, one-step growth curve and sensitivity to temperature and pH of bacteriophage PF23 were observed by electron microscope, the bacteriophage genome was extracted, the nucleic acid type was identified by enzyme digestion, and the genome size was estimated preliminarily. The model of systemic infection in mice was established and the therapeutic effect of PF23 was observed. Results the plaque of PF23 was halo clear and transparent round spot with a diameter of about 2 mm. The morphological characteristics of PF23 were observed by electron microscope, and its stability was good at 50 鈩,

本文編號(hào):2487848

資料下載
論文發(fā)表

本文鏈接:http://sikaile.net/huliyixuelunwen/2487848.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶7a334***提供,本站僅收錄摘要或目錄,作者需要?jiǎng)h除請(qǐng)E-mail郵箱bigeng88@qq.com
国产一区二区三区色噜噜| 东京热男人的天堂社区| 成人国产激情在线视频| 日韩日韩日韩日韩在线| 亚洲国产精品久久琪琪| 日韩性生活视频免费在线观看| 国产成人精品一区在线观看 | 老司机精品视频在线免费看 | 久久这里只有精品中文字幕| 日韩黄片大全免费在线看 | 国产精品欧美激情在线观看| 中国美女偷拍福利视频| 亚洲国产综合久久天堂| 国产人妻熟女高跟丝袜| 亚洲性日韩精品一区二区| 热情的邻居在线中文字幕| 香蕉尹人视频在线精品| 久久精品亚洲欧美日韩| 亚洲av又爽又色又色| 亚洲一区二区三区三区| 深夜少妇一区二区三区| 黄色污污在线免费观看| 亚洲最新av在线观看| 开心五月激情综合婷婷色| 中文字幕亚洲在线一区| 日韩精品区欧美在线一区| 亚洲一区二区三区在线免费| 日韩欧美黄色一级视频| 亚洲国产成人av毛片国产 | 亚洲欧美国产精品一区二区| 国产亚洲精品一二三区| 亚洲黄片在线免费小视频| 热情的邻居在线中文字幕| 中国美女草逼一级黄片视频| 九九热在线免费在线观看| 日本人妻中出在线观看| 欧美午夜视频免费观看| 亚洲最新av在线观看| 视频一区二区三区自拍偷| 日本午夜福利视频免费观看| 亚洲精品中文字幕无限乱码|