【摘要】：目的探討超早期高壓氧治療對脊髓全橫斷損傷大鼠股骨骨鈣、骨生物力學(xué)及骨膠原的影響。方法 75只Sprague-Dawley大鼠隨機(jī)分為假手術(shù)組(n=15)、模型組(n=20)和高壓氧組(n=40),其中高壓氧組又分為3小時組(高壓氧1組,n=20)和12小時組(高壓氧2組,n=20)。假手術(shù)組僅行椎板切除術(shù),其他三組均行T10水平脊髓完全橫向切斷術(shù)。高壓氧1組和高壓氧2組分別于術(shù)后第3、12小時開始高壓氧治療,10 d為1個療程,共3個療程。治療后,各組取右股骨測定股骨生物力學(xué)性能參數(shù),測定骨鈣、骨羥脯氨酸(Hyp)含量;HE染色和Masson染色觀察骨小梁和骨膠原形態(tài)學(xué)變化。結(jié)果治療后,與假手術(shù)組比較,模型組股骨結(jié)構(gòu)力學(xué)和材料力學(xué)參數(shù)下降(P0.05),骨干重、骨鈣及Hyp含量減少(P0.05);骨小梁及骨膠原數(shù)目減少,稀疏、斷裂、排列不整。與模型組和高壓氧2組比較,高壓氧1組股骨結(jié)構(gòu)力學(xué)和材料力學(xué)參數(shù)均增高,骨干重、骨鈣及Hyp含量增加(P0.05),骨小梁及骨膠原數(shù)目增多,連續(xù)性較好,排列較整齊。結(jié)論超早期高壓氧治療可明顯改善脊髓全橫斷損傷大鼠股骨骨鈣含量,改善骨膠原含量及質(zhì)量,改善生物力學(xué)性能。
[Abstract]:Aim to investigate the effects of hyperbaric oxygen therapy on calcium, bone biomechanics and collagen of femur in rats with spinal cord transection injury. Methods 75 Sprague-Dawley rats were randomly divided into three groups: sham operation group (n = 15), model group (n = 20) and hyperbaric oxygen group (n = 40). The rats in hyperbaric oxygen group were divided into 3 hours group (hyperbaric oxygen group 1, n = 20) and 12 hours group (hyperbaric oxygen group 2, n = 20). In the sham operation group, laminectomy was performed only, and the other three groups underwent complete transverse spinal cord resection at T 10 level. Hyperbaric oxygen group 1 and hyperbaric oxygen group 2 began hyperbaric oxygen therapy at 3 and 12 hours after operation respectively, 10 days as a course of treatment, a total of 3 courses. After treatment, the biomechanical parameters of femur, bone calcium and hydroxyproline (Hyp) content were measured, and the morphological changes of trabecular bone and bone collagen were observed by HE staining and Masson staining. Results after treatment, compared with the sham-operated group, the parameters of femoral structural mechanics and material mechanics in the model group decreased (P0.05), bone mass, bone calcium and Hyp decreased (P0.05). The number of trabeculae and collagen decreased, sparse, fractured, and irregular. Compared with model group and hyperbaric oxygen group, the parameters of femoral structural mechanics and material mechanics, bone mass, bone calcium and Hyp content, bone trabecular and collagen number, continuity and arrangement in hyperbaric oxygen group 1 were higher than those in model group and hyperbaric oxygen group (P0.05). Conclusion Hyperbaric oxygen therapy can significantly improve the calcium content, collagen content and biomechanical properties of femur in rats with spinal cord transection injury.
【作者單位】： 中南大學(xué)湘雅二醫(yī)院;
【基金】：湖南省科技廳基金項目(No.2008SK3108)
【分類號】：R651.2;R459.6
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本文編號：2456382