血液核酸篩查非特異擴增曲線的原因分析
發(fā)布時間：2019-03-13 11:22
【摘要】：目的分析血液篩查Roche Cobas s201核酸檢測系統(tǒng)非特異擴增曲線產(chǎn)生的原因。方法統(tǒng)計本室2014年10月-2015年12月用Cobas Taq Screen MPX v2.0核酸試劑檢測產(chǎn)生非特異擴增曲線的次數(shù),探討其發(fā)生與時間、檢測系統(tǒng)(A、B和C系統(tǒng))和試劑批號(批號1、批號2、批號3、批號4、批號5和批號6)之間的關(guān)系。結(jié)果本室核酸檢測非特異擴增曲線總發(fā)生率為0.386%(86/2 228);不同月份(χ~2=27.325,P0.05)、不同檢測系統(tǒng)(χ~2=66.305,P0.05)和不同批號試劑(χ~2=129.550,P0.05)之間的非特異擴增曲線發(fā)生率均有差異,其中2015年2月份、C檢測系統(tǒng)和試劑批號2的發(fā)生率在各分項中均最大,分別為0.893%(10/1 120)、3.17%(10/315)和5.26%(10/190);非特異擴增曲線類型中以HCV曲線出現(xiàn)47次最多,占54.65%(47/86)。結(jié)論核酸檢測非特異擴增曲線的發(fā)生與檢測系統(tǒng)和試劑批號有明顯相關(guān)性,加強對檢測系統(tǒng)的管理和試劑確認(rèn)管理能夠提高核酸檢測性能。
[Abstract]:Objective to analyze the causes of nonspecific amplification curve of Roche Cobas S201 nucleic acid detection system in blood screening. Methods from October 2014 to December 2015, the number of non-specific amplification curves produced by Cobas Taq Screen MPX v2.0 nucleic acid reagent was counted, and its occurrence and time were discussed. The detection system (A, B and C systems) and the batch number (lot number 1, B and C) were used to detect the non-specific amplification curve. The relationship between lot number 2, batch number 3, batch number 4, batch number 5 and batch number 6). Results the total incidence of nonspecific amplification curve was 0.386% (86 渭 2.228). The incidence of non-specific amplification curves in different months (蠂 ~ 2 / 27.325, P 0.05), different detection systems (蠂 ~ 2 / 66.305, P 0.05) and different batches of reagents (蠂 ~ 2 / 129.550, P 0.05) were all different, in which February 2015, the incidence of non-specific amplification curve was significantly different. The incidence of C detection system and assay number 2 was the highest in all sub-items, which were 0.893% (10 / 120), 3.17% (10 / 315) and 5.26% (10 / 190), respectively. Among the non-specific amplification curve types, the HCV curve appeared 47 times, accounting for 54.65% (47 / 86). Conclusion the occurrence of non-specific amplification curve of nucleic acid detection has obvious correlation with detection system and reagent batch number. Strengthening the management of detection system and reagent confirmation management can improve the performance of nucleic acid detection.
【作者單位】： 東莞市中心血站;
【基金】：東莞市醫(yī)療衛(wèi)生類科技計劃一般項目(2015105101245)
【分類號】：R440
，
本文編號：2439344
[Abstract]:Objective to analyze the causes of nonspecific amplification curve of Roche Cobas S201 nucleic acid detection system in blood screening. Methods from October 2014 to December 2015, the number of non-specific amplification curves produced by Cobas Taq Screen MPX v2.0 nucleic acid reagent was counted, and its occurrence and time were discussed. The detection system (A, B and C systems) and the batch number (lot number 1, B and C) were used to detect the non-specific amplification curve. The relationship between lot number 2, batch number 3, batch number 4, batch number 5 and batch number 6). Results the total incidence of nonspecific amplification curve was 0.386% (86 渭 2.228). The incidence of non-specific amplification curves in different months (蠂 ~ 2 / 27.325, P 0.05), different detection systems (蠂 ~ 2 / 66.305, P 0.05) and different batches of reagents (蠂 ~ 2 / 129.550, P 0.05) were all different, in which February 2015, the incidence of non-specific amplification curve was significantly different. The incidence of C detection system and assay number 2 was the highest in all sub-items, which were 0.893% (10 / 120), 3.17% (10 / 315) and 5.26% (10 / 190), respectively. Among the non-specific amplification curve types, the HCV curve appeared 47 times, accounting for 54.65% (47 / 86). Conclusion the occurrence of non-specific amplification curve of nucleic acid detection has obvious correlation with detection system and reagent batch number. Strengthening the management of detection system and reagent confirmation management can improve the performance of nucleic acid detection.
【作者單位】： 東莞市中心血站;
【基金】：東莞市醫(yī)療衛(wèi)生類科技計劃一般項目(2015105101245)
【分類號】：R440
，
本文編號：2439344
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