發(fā)布時(shí)間：2019-02-27 17:48

【摘要】：對(duì)從河北某些經(jīng)濟(jì)昆蟲養(yǎng)殖場(chǎng)發(fā)病(死亡)蝗蟲(東亞飛蝗)中分離的褪色沙雷氏菌(Serratia marcescens),進(jìn)行了比較系統(tǒng)的主要生物學(xué)性狀的研究,主要包括形態(tài)學(xué)特征、理化特性、系統(tǒng)發(fā)育學(xué)、病原學(xué)意義、血清學(xué)分型、脈沖場(chǎng)凝膠電泳分子分型等方面的內(nèi)容。研究結(jié)果顯示,來(lái)源于發(fā)病(死亡)養(yǎng)殖蝗蟲的褪色沙雷氏菌,各菌株具有相同的基本表觀生物學(xué)指征性狀和系統(tǒng)發(fā)育特征,但存在產(chǎn)生、不產(chǎn)生紅色色素的菌株。通過(guò)對(duì)健康蝗蟲的回歸感染實(shí)驗(yàn),按 科赫法則‖進(jìn)行病原學(xué)意義判定,表明分離菌株具有比較強(qiáng)的致病作用,也是構(gòu)成蝗蟲自然發(fā)病(死亡)的原發(fā)病原菌。選擇從不同蝗蟲養(yǎng)殖場(chǎng)分離獲得的病原褪色沙雷氏菌代表菌株,采用經(jīng)典的免疫血清學(xué)凝集試驗(yàn)方法進(jìn)行血清學(xué)同源性分析,表明在不同菌株間存在同源性差異性,但均存在顯著的血清學(xué)交叉反應(yīng)。采用脈沖場(chǎng)凝膠電泳方法對(duì)不同菌株進(jìn)行分子分型研究,結(jié)果供試11株褪色沙雷氏菌的基因相似性在64%~100%之間,相互存在不同程度的親緣關(guān)系。本研究表明,來(lái)源于養(yǎng)殖蝗蟲的褪色沙雷氏菌野生菌株,具有比較典型的褪色沙雷氏菌生物學(xué)性狀和系統(tǒng)發(fā)育特征,但存在不同的抗原特異性,脈沖場(chǎng)凝膠電泳分析存在一定程度的親緣關(guān)系,對(duì)養(yǎng)殖蝗蟲具有重要的病原學(xué)意義。這些,不僅豐富了褪色沙雷氏菌的生物學(xué)性狀內(nèi)容,也為對(duì)褪色沙雷氏菌的檢驗(yàn)與進(jìn)一步深入研究提供了具有參考價(jià)值的科學(xué)資料。另一方面是鑒于褪色沙雷氏菌并非人類及養(yǎng)殖動(dòng)物的重要病原菌,可能會(huì)成為對(duì)蝗災(zāi)進(jìn)行生物學(xué)防治研究的直接或構(gòu)建基因工程菌的候選菌株。
[Abstract]:The main biological characters of (Serratia marcescens), isolated from locusts (locusts) isolated from some economic insect farms in Hebei province were studied systematically, including morphological characteristics, physicochemical characteristics, and physical and chemical characteristics. Phylogeny, etiological significance, serological typing, pulse field gel electrophoresis molecular typing and so on. The results showed that the strains from the diseased (dead) cultured locusts had the same basic epibiological characters and phylogenetic characteristics, but there were strains that produced no red pigment. Through the regression infection experiment of healthy locusts, the etiological significance was determined according to the rule of Koch method. The results showed that the isolated strains had strong pathogenic effect and were also the primary pathogenic bacteria of locusts' natural disease (death). The representative strains isolated from different locust farms were selected and the classical serological agglutination test was used to analyze the serological homology. The results showed that there were homology differences among the different strains. However, there were significant serological cross-reactions. The molecular typing of different strains was studied by pulsed field gel electrophoresis (PFGE). The results showed that the genetic similarity of 11 strains of Serratia discolor was between 64% and 100%, and there was different degree of genetic relationship between them. The results showed that the wild strain of Serratia discolor derived from cultured locusts had typical biological and phylogenetic characteristics, but had different antigen specificity. Pulsed-field gel electrophoresis (PFGE) analysis has a certain degree of phylogenetic relationship, which is of great significance for culturing locusts. These results not only enrich the biological characters of Serratia discolor, but also provide valuable scientific data for the examination and further study of Serratia discolor. On the other hand, Serratia discolor is not an important pathogen in human and farmed animals, so it may become a candidate strain to study the biological control of locust infestation directly or to construct genetically engineered bacteria.
【學(xué)位授予單位】：河北科技師范學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R446.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前5條

1 尚艷霞，，張麗娜;粘質(zhì)沙雷氏菌感染5例臨床分析[J];河北醫(yī)藥;1994年02期

2 馮嘉麗;胡靜;陳清;;香港海鷗形菌DNA脈沖場(chǎng)凝膠電泳的技術(shù)條件[J];熱帶醫(yī)學(xué)雜志;2010年10期

3 馮廣達(dá);鄧名榮;郭俊;朱紅惠;羊宋貞;梁浩亮;朱昌雄;;廣東某農(nóng)村塘壩飲用水污染的微生物溯源[J];中國(guó)環(huán)境科學(xué);2011年01期

4 葉蕊;石麗媛;王鵬;宋志忠;;脈沖場(chǎng)凝膠電泳技術(shù)簡(jiǎn)介及其在細(xì)菌分子分型中的應(yīng)用[J];中國(guó)媒介生物學(xué)及控制雜志;2013年02期

5 呂國(guó)平;李麗婕;蘆飛;徐保紅;劇慧棟;郭玉梅;秦麗云;;食源性金黃色葡萄球菌脈沖場(chǎng)凝膠電泳分型分析[J];微生物學(xué)雜志;2013年04期

本文編號(hào)：2431475