【摘要】：目的通過(guò)Carba NP試驗(yàn)對(duì)革蘭陰性菌產(chǎn)生的碳青霉烯酶進(jìn)行快速檢測(cè),了解臨床產(chǎn)碳青霉烯酶的革蘭陰性菌的臨床分布及耐藥特征,并驗(yàn)證CarbaNP試驗(yàn)準(zhǔn)確性,為臨床合理使用抗菌藥物提供理論依據(jù)。方法選取醫(yī)院2013年10月-2014年10月檢出1 135株革蘭陰性菌,利用Carba NP比色法對(duì)其中90株多耐藥菌產(chǎn)碳青霉烯酶進(jìn)行檢測(cè),并通過(guò)改良Hodge試驗(yàn),亞胺培南-EDTA(乙二胺四乙酸)雙紙片協(xié)同實(shí)驗(yàn)等方法驗(yàn)證其準(zhǔn)確性、特異性。結(jié)果肺炎克雷伯菌、鮑氏不動(dòng)桿菌主要來(lái)自呼吸道標(biāo)本,共693株,占61.06%,銅綠假單胞菌主要來(lái)自痰液及燒傷創(chuàng)面膿汁,共425株,占37.44%,1 135株革蘭陰性菌篩選出多耐藥菌為90株,占7.93%,將90株多耐藥的菌株經(jīng)Carba NP法檢測(cè)出產(chǎn)碳青霉烯酶株22株。結(jié)論 Carba NP試驗(yàn)用于檢測(cè)碳青霉烯酶操作簡(jiǎn)單、快速、靈敏、費(fèi)用低廉、特異性強(qiáng),可有助于提高產(chǎn)碳青霉烯酶革蘭陰性菌的檢出率和抗菌藥物的合理使用水平。
[Abstract]:Objective to investigate the clinical distribution and drug resistance of gram-negative bacteria producing carbapenem by Carba NP test, and to verify the accuracy of CarbaNP test. To provide a theoretical basis for the rational use of antimicrobial drugs in clinic. Methods from October 2013 to October 2014, 1 135 strains of Gram-negative bacteria were detected by Carba NP colorimetry, and 90 strains of multidrug-resistant strains were detected by Hodge test. The accuracy and specificity of imipenem-EDTA (ethylenediamine tetraacetic acid) double disk synergy test were verified. Results Klebsiella pneumoniae and Acinetobacter baumannii mainly came from respiratory tract specimens, 693 strains (61.06%). Pseudomonas aeruginosa mainly came from sputum and burn wound pus, 425 strains (37.44%). 90 strains (7.933%) of multidrug resistant strains were screened out from 1 135 strains of Gram-negative bacteria. 22 strains of carbapenem producing strains were detected by Carba NP. Conclusion the Carba NP test is simple, rapid, sensitive, inexpensive and specific for the detection of carbapenem. It is helpful to improve the detection rate of carbapenem producing gram-negative bacteria and the level of rational use of antimicrobial agents.
【作者單位】： 沈陽(yáng)醫(yī)學(xué)院;遼寧省環(huán)境污染與微生態(tài)研究室;
【基金】：遼寧省科學(xué)事業(yè)公益研究基金資助項(xiàng)目(GY2013-A-014) 遼寧省大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練基金資助項(xiàng)目(20150810)
【分類號(hào)】：R446.5

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