【摘要】：目的:探討運(yùn)動(dòng)干預(yù)對實(shí)驗(yàn)性高同型半胱氨酸血癥(hyperhomocysteinemia,HHcy)大鼠過氧化及血管內(nèi)皮功能的影響。方法:將48只大鼠隨機(jī)分為6組,每組各8只。對照組(n=8只)給予普通飼料喂養(yǎng);高蛋氨酸組(n=40只)在此基礎(chǔ)上加3%的蛋氨酸,再根據(jù)運(yùn)動(dòng)強(qiáng)度分為模型組、葉酸組、HHcy運(yùn)動(dòng)組(低強(qiáng)度組、中強(qiáng)度組、高強(qiáng)度組),8只/組,持續(xù)16周。檢測各組大鼠血清同型半胱氨酸(Hcy)、血漿丙二醛(MDA)含量和谷胱甘肽過氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)活性,測定一氧化氮(NO)、一氧化氮合酶(NOS)、內(nèi)皮素1(ET-1)水平。結(jié)果:干預(yù)后8周,HHcy運(yùn)動(dòng)組血清Hcy水平明顯下降,且顯著低于模型組(16.09±4.85 vs.24.24±3.11μmol/L)(P0.05);干預(yù)后16周,HHcy運(yùn)動(dòng)組血清Hcy水平與對照組無顯著性差異(7.81±0.64 vs.6.63±1.27μmol/L)(P0.05),各組血清Hcy水平依次為葉酸組(6.63±1.27μmol/L)低強(qiáng)度組(7.62±0.73μmol/L)中強(qiáng)度組(6.91±0.37μmol/L)高強(qiáng)度組(6.19±0.34μmol/L)(P0.05);干預(yù)后16周,HHcy運(yùn)動(dòng)組MDA均顯著下降,SOD活力和GSH-PX含量明顯升高(P0.05),中、高強(qiáng)度運(yùn)動(dòng)組各過氧化指標(biāo)與對照組無顯著性差異(P0.05);與對照組比較,干預(yù)后16周模型組、葉酸組和低強(qiáng)度組ET-1明顯升高(61.64±12.29、58.86±10.32、59.11±9.09 vs 49.48±11.09pg/ml),NOS(8.52±2.09、18.13±4.65、17.08±4.20 vs 33.98±6.67U/ml)、NO(1.19±0.24、1.30±0.41、1.30±0.41 vs 2.29±0.28μg/L)明顯下降(P0.05),而中強(qiáng)度組、高強(qiáng)度組中ET-1、NOS與對照組比較,差異無顯著性意義(P0.05);HHcy運(yùn)動(dòng)組、葉酸組NO均明顯高于模型組(P0.05)。結(jié)論:高蛋氨酸飲食可誘發(fā)大鼠HHcy,而中、高強(qiáng)度運(yùn)動(dòng)干預(yù)有助于降低HHcy大鼠血清Hcy水平,改善氧化應(yīng)激狀態(tài),減輕內(nèi)皮功能損傷。
[Abstract]:Aim: to investigate the effects of exercise intervention on peroxidation and vascular endothelial function in experimental hyperhomocysteinemia (hyperhomocysteinemia,HHcy) rats. Methods: 48 rats were randomly divided into 6 groups with 8 rats in each group. The control group (n = 8) was fed with common feed. High methionine group (n = 40) added 3% methionine on this basis. According to exercise intensity, it was divided into three groups: model group, folic acid group, HHcy exercise group (low intensity group, moderate intensity group, high intensity group), 8 rats in each group for 16 weeks. The contents of plasma malondialdehyde (MDA), glutathione peroxidase (GSH-PX), superoxide dismutase (SOD) and nitric oxide (NO), nitric oxide synthase (NOS),) were measured in serum homocysteine (Hcy), of rats in each group. Endothelin 1 (ET-1) level. Results: at 8 weeks after intervention, the serum Hcy level in HHcy exercise group was significantly lower than that in model group (16.09 鹵4.85 vs.24.24 鹵3.11 渭 mol/L) (P0.05). At 16 weeks after intervention, there was no significant difference in serum Hcy level between HHcy exercise group and control group (7.81 鹵0.64 vs.6.63 鹵1.27 渭 mol/L) (P0.05). The serum Hcy levels of folic acid group (6.63 鹵1.27 渭 mol/L), low intensity group (7.62 鹵0.73 渭 mol/L), moderate intensity group (6.91 鹵0.37 渭 mol/L), high intensity group (6.19 鹵0.34 渭 mol/L) (P0.05); At 16 weeks after intervention, MDA decreased significantly in HHcy exercise group, SOD activity and GSH-PX content increased significantly (P0.05), and there was no significant difference in peroxidation index between high intensity exercise group and control group (P0.05). Compared with the control group, ET-1 in the folic acid group and low intensity group increased significantly (61.64 鹵12.2958.86 鹵10.32 vs 59.11 鹵9.09 vs 49.48 鹵11.09pg/ml) 16 weeks after intervention. NOS (8.52 鹵2.09 鹵4.65 鹵17.08 鹵4.20 vs 33.98 鹵0.24 6.67U/ml), NO (1.30 鹵0.41 vs 2.29 鹵0.28 渭 g / L) decreased significantly (P0.05), while in the medium intensity group, ET-1, in the high intensity group decreased significantly (P0.05). There was no significant difference between NOS and control group (P0.05). NO in HHcy exercise group and folic acid group were significantly higher than that in model group (P0.05). Conclusion: high methionine diet can induce HHcy, in rats. High intensity exercise intervention can reduce the level of serum Hcy in HHcy rats, improve the state of oxidative stress, and alleviate the injury of endothelial function.
【作者單位】： 浙江醫(yī)院心臟康復(fù)中心;
【基金】：浙江省自然科學(xué)基金資助項(xiàng)目(LY12H17001)
【分類號】：R54;R493

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 朱紅芳;潘群;;社區(qū)運(yùn)動(dòng)干預(yù)對2型糖尿病患者的影響[J];中國臨床護(hù)理;2010年02期

2 韓俏英;梁繼娟;李蕾;;飲食控制和運(yùn)動(dòng)干預(yù)對糖尿病患者康復(fù)的影響[J];現(xiàn)代臨床護(hù)理;2009年05期

3 張學(xué)慧;;運(yùn)動(dòng)干預(yù)對2型糖尿病病人血管功能的影響[J];護(hù)理研究;2012年16期

4 朱立新;;社區(qū)青年高血壓運(yùn)動(dòng)干預(yù)中期效果分析[J];基層醫(yī)學(xué)論壇;2012年23期

5 劉國環(huán);王淑文;;運(yùn)動(dòng)干預(yù)對老年2型糖尿病病人血糖血脂的影響[J];臨床醫(yī)學(xué)工程;2013年09期

6 付延玉;羅紅超;廖曉玲;劉麗玉;張本菊;;飲食和運(yùn)動(dòng)干預(yù)對非酒精性脂肪肝病患者疾病轉(zhuǎn)歸的影響[J];現(xiàn)代臨床護(hù)理;2014年01期

7 王莉;孫中莉;;高脂血癥85例飲食和運(yùn)動(dòng)干預(yù)[J];齊魯護(hù)理雜志;2008年11期

8 李娟,李青,常仁翠;運(yùn)動(dòng)干預(yù)對老年2型糖尿病病人血糖血脂的影響[J];護(hù)理學(xué)雜志;2003年09期

9 吳云海;;糖尿病康復(fù)中運(yùn)動(dòng)干預(yù)作用的相關(guān)性研究[J];實(shí)用中醫(yī)內(nèi)科雜志;2008年07期

10 王玉俠;王人衛(wèi);;運(yùn)動(dòng)干預(yù)對癌癥發(fā)生、患者生存及預(yù)后的影響[J];中國運(yùn)動(dòng)醫(yī)學(xué)雜志;2011年04期

相關(guān)會議論文 前1條

1 馮鵬程;楊寶國;余永章;;運(yùn)動(dòng)干預(yù)在戒毒矯治中的應(yīng)用研究[A];中國康復(fù)醫(yī)學(xué)會運(yùn)動(dòng)療法分會第十一屆全國康復(fù)學(xué)術(shù)大會學(xué)術(shù)會議論文摘要匯編[C];2011年

相關(guān)碩士學(xué)位論文 前1條

1 劉愛鋒;運(yùn)動(dòng)干預(yù)對社區(qū)中老年原發(fā)性高血壓病人的降壓效果分析[D];延邊大學(xué);2007年

，

本文編號：2365758