【摘要】：目的排除HIV血清學(xué)篩查中的假陽性獻(xiàn)血者,探討HIV血清學(xué)反應(yīng)性獻(xiàn)血者的歸隊(duì)條件。方法對(duì)2013年1月1日-2015年6月30日間大連市血液中心常規(guī)血液篩查中核酸檢測(cè)(NAT)無反應(yīng)性、HIV血清學(xué)反應(yīng)性且不被免疫印跡法(WB)確證的獻(xiàn)血者做歸隊(duì)復(fù)查。復(fù)查方式同血液篩查,但血清學(xué)檢測(cè)不設(shè)"灰區(qū)"。結(jié)果共篩查出HIV血清學(xué)反應(yīng)性且確證陽性83例,流行率為0.43‰(83/192 065),其中第3代ELISA漏檢4例(漏檢率0.2/萬),第4代ELISA漏檢2例(漏檢率0.1/萬);ELISA試劑設(shè)置"灰區(qū)"的特異性低于取消"灰區(qū)"的特異性(特異性差D的95%可信區(qū)間:第3代試劑0.06‰-0.17‰,第4代試劑0.18‰-0.32‰);研究期間歸隊(duì)復(fù)查獻(xiàn)血者68人,歸隊(duì)檢測(cè)總次數(shù)為112次,單次歸隊(duì)復(fù)查的時(shí)間跨度為47-867(中位數(shù)194)d,單人歸隊(duì)的時(shí)間總跨度為89-908(中位數(shù)373)d。41人僅做了1次復(fù)查,合格率為70.73%(29/41);14人做了2次復(fù)查,合格率為57.14%(8/14);10人做了3次復(fù)查,90.00%(9/10);3人做了≥4次復(fù)查,合格率為33.33%(1/3)。歸隊(duì)反應(yīng)性標(biāo)本的確證試驗(yàn)全部為陰性。結(jié)論采用NAT和第4代ELISA聯(lián)合檢測(cè)模式同時(shí)取消HIV血清學(xué)檢測(cè)的"灰區(qū)"可以減少血液篩查及歸隊(duì)檢測(cè)中的假陽性,利于更多的獻(xiàn)血者歸隊(duì);血清學(xué)HIV檢測(cè)反應(yīng)性獻(xiàn)血者的歸隊(duì)標(biāo)準(zhǔn)宜為每次復(fù)查間隔時(shí)間≥3個(gè)月,2次復(fù)查合格者即可再次獻(xiàn)血。
[Abstract]:Objective to exclude false positive blood donors from HIV serological screening and to explore the conditions of return of HIV serological reactive donors. Methods from January 1, 2013 to June 30, 2015, donors whose nucleic acids were tested for (NAT) nonreactivity and HIV serological reactivity and were not confirmed by Western blot (WB) were reviewed. The method of reexamination was the same as blood screening, but no gray area was set up for serological examination. Results 83 cases of HIV seroreactivity were screened and confirmed positive, the prevalence rate was 0.43 鈥，

本文編號(hào)：2333736