【摘要】：目的:觀察環(huán)保固定液(聚羥基丙烯酸、環(huán)保透明脫蠟液Van-clear單獨(dú)或聯(lián)合)替代傳統(tǒng)固定液[4%(體積分?jǐn)?shù))中性緩沖甲醛、傳統(tǒng)透明脫蠟液二甲苯]應(yīng)用于熒光原位雜交(fluorescence in situ hybridization,FISH)法檢測(cè)宮頸組織中人端粒酶核糖核酸組分(human telomerase RNA component,h TERC)基因擴(kuò)增的差異。方法:收集2013年3月到2015年4月于中山市博愛醫(yī)院婦科住院部送檢的255例宮頸組織標(biāo)本,同一病變部位切取4個(gè)樣本,分為4組,命名為A、B、C、D組:A組采用4%中性緩沖甲醛固定、二甲苯透明脫蠟制作切片;B組采用聚羥基丙烯酸固定、二甲苯透明脫蠟制作切片;C組采用4%中性緩沖甲醛固定、Van-clear透明脫蠟制作切片;D組采用聚羥基丙烯酸固定、Van-clear透明脫蠟制作切片。采用FISH技術(shù)檢測(cè)4組宮頸標(biāo)本中h TERC基因。結(jié)果:在FISH法檢測(cè)宮頸各級(jí)病變組織h TERC基因時(shí),熒光顯微鏡下,A、B、C、D四組的組織輪廓和背景均清晰,探針定位準(zhǔn)確,可見耀眼的紅/綠熒光信號(hào)。B、C、D組與A組陽(yáng)性率相比差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05),且FISH結(jié)果符合率高。結(jié)論:環(huán)保試劑聚羥基丙烯酸、Van-clear有潛在的可能單獨(dú)或聯(lián)合替代4%中性緩沖甲醛、二甲苯應(yīng)用于FISH法檢測(cè)宮頸h TERC基因。
[Abstract]:Objective: to observe the substitution of Van-clear (polyhydroxy acrylic acid, environmental transparent dewaxing solution Van-clear) for the traditional fixed solution [4% (volume fraction)] neutral buffer formaldehyde. Traditional transparent dewaxing solution xylene was used in fluorescence in situ hybridization (fluorescence in situ hybridization,FISH) method to detect the difference of amplification of human telomerase RNA (human telomerase RNA component,h TERC) gene in cervical tissues. Methods: from March 2013 to April 2015, 255 specimens of cervical tissue were collected from the department of gynecology of Boai Hospital, Zhongshan City. Four samples were taken from the same lesion site and divided into 4 groups, named as Group A: group A, fixed with 4% neutral buffered formaldehyde. The transparent dewaxing of xylene was used in group B to be fixed with polyhydroxyacrylic acid. The transparent dewaxing of xylene in group C was made with 4% neutral buffered formaldehyde fixed Van-clear transparent dewaxing. Group D used polyhydroxyacrylic acid to fix Van-clear transparent dewaxing. H TERC gene in 4 groups of cervical specimens was detected by FISH technique. Results: when the h TERC gene was detected by FISH, the tissue profile and background of the four groups were clear and the probe location was accurate. It can be seen that there was no significant difference in the positive rate between group D and group A (P0.05), and the coincidence rate of FISH results was high. Conclusion: Van-clear, an environmental protection reagent, has the potential to replace 4% neutral buffered formaldehyde alone or in combination. Xylene is used to detect h TERC gene in cervix by FISH.
【作者單位】： 南方醫(yī)科大學(xué)附屬中山博愛醫(yī)院病理科;
【基金】：中山市衛(wèi)生局醫(yī)學(xué)科研立項(xiàng)課題(2014J128)資助~~
【分類號(hào)】：R440

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