耐甲氧西林金黃色葡萄球菌pvl及tst基因分布與感染類型研究
發(fā)布時(shí)間：2018-09-17 10:29
【摘要】：目的了解耐甲氧西林金黃色葡萄球菌(MRSA)臨床菌株攜帶殺白細(xì)胞毒素(PVL)編碼基因pvl和中毒休克綜合征毒素-1(TSST-1)編碼基因tst情況、流行特征及感染類型,為該類感染的臨床治療提供依據(jù)。方法收集2012年1-12月臨床分離的MRSA 96株,采用PCR檢測(cè)96株MRSA中pvl和tst基因,采用全自動(dòng)微生物分析儀檢測(cè)11種臨床常用抗菌藥物的敏感性,采用多重PCR對(duì)攜帶pvl基因(pvl+)或tst基因(tst+)的MRSA進(jìn)行mec分型(SCCmec),了解與分析pvl+或tst+MRSA感染類型。結(jié)果 96株MRSA中20株檢出pvl基因占20.8%,13株檢出tst基因占13.5%,未發(fā)現(xiàn)同時(shí)攜帶pvl和tst基因的菌株;除左氧氟沙星和莫西沙星外,pvl+/tst+MRSA對(duì)其他抗菌藥物耐藥率與pvl-/tst-菌株相似;20株pvl+MRSA中7株社區(qū)獲得性感染菌株、13株醫(yī)院獲得性感染菌株,其中17株分離自皮膚和軟組織感染患者膿液,13株tst+MRSA均為醫(yī)院獲得感染菌株,其中8株分離自肺部感染患者痰液;20株pvl+MRSA中,SCCmecⅢ型11株、SCCmecⅣa型5株、SCCmecII型2株,2株未能分型;13株tst+MRSA均為SCCmecⅢ型。結(jié)論 pvl+MRSA在社區(qū)和醫(yī)院內(nèi)均有流行,主要引起皮膚和軟組織感染;tst+MRSA僅在醫(yī)院內(nèi)流行,主要引起肺部感染;pvl+和tst+MRSA均以SCCmecⅢ型為主。
[Abstract]:Objective to investigate the prevalence, prevalence and type of infection in clinical strains of methicillin-resistant Staphylococcus aureus (MRSA) carrying leukocytotoxin (PVL) coding gene (pvl) and toxic shock syndrome toxin 1 (TSST-1) gene tst. To provide the basis for the clinical treatment of this kind of infection. Methods 96 strains of MRSA isolated from January to December 2012 were collected. The genes of pvl and tst in 96 strains of MRSA were detected by PCR, and the sensitivity of 11 kinds of commonly used antimicrobial agents were detected by automatic microbiological analyzer. Mec typing of MRSA carrying pvl gene (pvl) or tst gene (tst) was conducted by multiple PCR and pvl or tst MRSA infection types were analyzed. Results out of 96 MRSA strains, 20 pvl genes were detected in 20.8G and 13 tst genes were detected in 13 strains, and no pvl and tst gene carriers were found. With the exception of levofloxacin and moxifloxacin, PVL / TST MRSA was resistant to other antimicrobial agents, which were similar to those of pvl-/tst- strains, including 7 community-acquired infections (pvl MRSA) and 13 nosocomial infections (Nosocomial). Among them, 17 strains were isolated from patients with skin and soft tissue infection and 13 strains of tst MRSA were nosocomial infected strains. Among the 20 sputum strains isolated from patients with pulmonary infection, 11 strains of SCCmec 鈪，
本文編號(hào)：2245562
[Abstract]:Objective to investigate the prevalence, prevalence and type of infection in clinical strains of methicillin-resistant Staphylococcus aureus (MRSA) carrying leukocytotoxin (PVL) coding gene (pvl) and toxic shock syndrome toxin 1 (TSST-1) gene tst. To provide the basis for the clinical treatment of this kind of infection. Methods 96 strains of MRSA isolated from January to December 2012 were collected. The genes of pvl and tst in 96 strains of MRSA were detected by PCR, and the sensitivity of 11 kinds of commonly used antimicrobial agents were detected by automatic microbiological analyzer. Mec typing of MRSA carrying pvl gene (pvl) or tst gene (tst) was conducted by multiple PCR and pvl or tst MRSA infection types were analyzed. Results out of 96 MRSA strains, 20 pvl genes were detected in 20.8G and 13 tst genes were detected in 13 strains, and no pvl and tst gene carriers were found. With the exception of levofloxacin and moxifloxacin, PVL / TST MRSA was resistant to other antimicrobial agents, which were similar to those of pvl-/tst- strains, including 7 community-acquired infections (pvl MRSA) and 13 nosocomial infections (Nosocomial). Among them, 17 strains were isolated from patients with skin and soft tissue infection and 13 strains of tst MRSA were nosocomial infected strains. Among the 20 sputum strains isolated from patients with pulmonary infection, 11 strains of SCCmec 鈪，
本文編號(hào)：2245562
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