【摘要】：目的:比較3種檢測(cè)方法對(duì)肺炎支原體(MP)感染的臨床診斷效果及應(yīng)用價(jià)值。方法:對(duì)疑為呼吸系統(tǒng)MP感染的494例住院患兒留取痰液,用PCR熒光探針法進(jìn)行MP DNA檢測(cè),用被動(dòng)凝集法和間接免疫熒光法進(jìn)行血清抗MP抗體檢測(cè)。結(jié)果:PCR熒光探針法陽性299例(60.53%),被動(dòng)凝集法陽性290例(58.70%),間接免疫熒光法陽性242例(48.99%),PCR熒光探針法與被動(dòng)凝集法的陽性率無差別(χ2=0.341,P0.012 5),間接免疫熒光法與PCR熒光探針法(χ2=13.274,P0.012 5)、被動(dòng)凝集法(χ2=9.383,P0.012 5)陽性率相比,差異均有統(tǒng)計(jì)學(xué)意義。被動(dòng)凝集法陽性結(jié)果中滴度為40的患兒共77例,其中70例的另2種方法結(jié)果均為陽性。結(jié)論:間接免疫熒光法的檢測(cè)陽性率低于PCR熒光探針法與被動(dòng)凝集法。對(duì)于兒童,被動(dòng)凝集法靜脈血標(biāo)本的采集較PCR熒光探針法的痰標(biāo)本采集更為簡(jiǎn)便,可將被動(dòng)凝集法作為MP感染的門診篩查項(xiàng)目;被動(dòng)凝集法應(yīng)將滴度≥40的結(jié)果判定為陽性。
[Abstract]:Objective: to compare the clinical diagnostic effect and application value of three detection methods for mycoplasma pneumoniae (MP) infection. Methods: sputum was collected from 494 hospitalized children with suspected respiratory MP infection, MP DNA was detected by PCR fluorescence probe method, serum anti-MP antibody was detected by passive agglutination method and indirect immunofluorescence method. Results there were 299 cases (60.53%) positive by PCR fluorescence probe method, 290 cases (58.70%) positive by passive agglutination method, and 242cases (48.99%) positive by indirect immunofluorescence method. There was no difference between PCR fluorescence probe method and passive agglutination method (蠂 20.341U P 0.0125), indirect immunofluorescence assay and PCR fluorescence probe method (蠂 213.274U P 0.012). 5) the positive rate of the passive agglutination method (蠂 29.383 P 0.012 5) was higher than that of the control group. The difference was statistically significant. There were 77 children with 40 titers in the positive results of the passive agglutination method, of which the other two methods were all positive. Conclusion: the positive rate of indirect immunofluorescence assay is lower than that of PCR fluorescence probe method and passive agglutination method. For children, the collection of venous blood samples by passive agglutination method is more convenient than that of sputum samples collected by PCR fluorescence probe method. Passive agglutination method can be used as an outpatient screening item for MP infection, and the results of titer 鈮，

本文編號(hào)：2204874