【摘要】：目的比較EB病毒(EBV)感染患兒全血淋巴細胞和血清中EB病毒DNA(EBV-DNA)定量分析結果的關系,探討如何根據患兒感染狀態(tài)合理選擇臨床標本類型。方法收集醫(yī)院2015年9月-12月疑似EB病毒感染的患兒199例,分別取患兒的外周全血及血清,采用實時熒光PCR定量檢測EBV-DNA及酶聯(lián)免疫吸附法測定EBV-CA-IgM,數據采用SPSS 13.0軟件進行統(tǒng)計分析。結果全血淋巴細胞EBV-DNA陽性標本95例,陽性率為47.7%;血清EBV-DNA陽性標本36例,陽性率為18.1%;血清EBV-CA-IgM抗體陽性23例,陽性率為11.6%;當血清IgM抗體陰性時,兩種標本類型EBV-DNA陽性率差異有統(tǒng)計學意義(P0.01);將全血及血清EBV-DNA均為陽性的標本進行配對分析,定量結果呈正相關(P0.01)。結論全血中淋巴細胞的EBV-DNA陽性率及拷貝數均高于血清。臨床上可以根據患兒的病情來選擇合適的標本。
[Abstract]:Objective to compare the relationship between the quantitative analysis of EBV DNA (EBV-DNA) in whole blood lymphocytes and serum of children with EBV (EBV) infection and to explore how to select the clinical specimens according to the infective status of EBV in children with Epstein-Barr virus (EBV) infection. Methods 199 children with suspected Epstein-Barr virus infection from September to December 2015 were collected and their peripheral blood and serum were collected respectively. EBV-DNA and EBV-CA-IgM were quantitatively detected by real-time fluorescence PCR and enzyme linked immunosorbent assay (Elisa). The data were analyzed by SPSS 13.0 software. Results the positive rate of EBV-DNA in whole blood lymphocytes was 47.7%, the positive rate of serum EBV-DNA was 18.1%, the positive rate of EBV-CA-IgM antibody was 11.6% in 23 cases, and the positive rate was 11.6% when the serum IgM antibody was negative. The positive rate of EBV-DNA was significantly different between the two types of samples (P0.01), and the positive rate of EBV-DNA in whole blood and serum samples was analyzed by paired analysis, and the quantitative results were positively correlated (P0.01). Conclusion the positive rate and copy number of EBV-DNA in whole blood lymphocytes are higher than those in serum. The appropriate specimen can be selected clinically according to the patient's condition.
【作者單位】： 武漢市婦女兒童醫(yī)療保健中心檢驗部;
【基金】：國家自然科學基金資助項目(81201604) 湖北省自然科學基金資助項目(2012FFB05301)
【分類號】：R725.1;R440

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