【摘要】：目的:對獻血員血液進行更加完善的血型血清學(xué)檢測,減少由于獻血員原因造成的紅細胞成分血ABO同型交叉配血不相合,從而避免血型血清學(xué)檢測不合格的血發(fā)往臨床。方法:1研究對象所研究標本來自滄州市中心血站2014年采集的所有無償獻血者的標本共計65487例,按采集時間將標本分為兩個組:其中單月份(1,3,5,7,9,11月份)采集的標本的共計33046例作為對照組;雙月份(2,4,6,8,10,12月份)采集的標本共計32441例作為試驗組。2實驗方法對照組:ABO正反定型:正定型紅細胞與抗-A、抗-B反應(yīng),反定型試劑A細胞、B細胞與血漿反應(yīng)。試驗組:ABO正定型:正定型紅細胞與抗-A、抗-B、抗-A1,抗-AB、抗-Ig G、C3d;反定型A細胞、B細胞、O細胞、S1細胞、S2細胞、S3細胞與獻血員血漿反應(yīng)。結(jié)果:兩組實驗方法在A亞型(不包括A2亞型)檢出率方面有顯著性差異,試驗組可以檢出更多的A亞型。兩組實驗方法在B亞型和AB亞型鑒定方面無顯著性差異。兩組實驗方法在不規(guī)則抗體檢出率方面有顯著性差異,試驗組檢出了更多的不規(guī)則抗體及一定比例的直抗陽性的獻血員。兩組實驗在臨床退血方面比較有顯著性差異,試驗組檢測后發(fā)出的血液退血率同對照組比較顯著降低。結(jié)論:在做ABO血型正反定型時,正定型同時加入抗-A1、抗-AB試劑可以提高A亞型(不包含A2亞型)的檢出率,反定型加入O細胞和抗體篩選細胞大大提高了獻血員血清中Ig M型不規(guī)則抗體的檢出率。兩組試驗檢驗出的血液,試驗組發(fā)出的血液臨床退血率明顯低于對照組。直接抗人球蛋白試驗檢出了獻血員中直抗陽性的血液。從而避免交叉配血時出現(xiàn)抗體篩選陰性但配血主側(cè)凝集的現(xiàn)象。
[Abstract]:Objective: to improve the blood type serological test of blood donors, to reduce the mismatch of ABO homotypic cross matching caused by blood donors, so as to avoid blood group serological examination unqualified blood to clinical. Methods A total of 65487 blood donors were collected from Cangzhou Central Blood Station in 2014. The specimens were divided into two groups according to the time of collection: 33046 samples were collected in a single month (1 / 3) as control group; A total of 32441 samples were collected in December as control group (control group .2). The positive and negative typing of positive type erythrocytes and anti-A, anti-B reaction, anti-typing reagent A cell B cell reaction with plasma were observed in the control group (n = 2). The results were as follows: (1) in the experiment group, 2 cases were divided into two groups: control group (n = 2) and control group (n = 2). In the test group, the positive definite pattern of positive type ABO: positive red blood cell and anti--A-, anti--B-, anti--A1, anti--AB-AB, anti--Ig GG C3d; antitype A cell / B cell / S _ 1 cell / S _ 2 cell / S _ 3 cell / blood donor plasma reaction. Results: there was significant difference in the detection rate of A subtype (excluding A2 subtype) between the two groups, and more A subtypes could be detected in the experimental group. There was no significant difference in the identification of subtype B and AB between the two groups. There were significant differences in the detection rate of irregular antibodies between the two groups. More irregular antibodies and a certain proportion of direct anti-positive blood donors were detected in the test group. There was a significant difference between the two groups in terms of clinical blood loss, and the blood degenerative rate of the test group was significantly lower than that of the control group. Conclusion: when ABO blood group is positive and negative, anti-A _ 1 is added into positive typing at the same time. Anti-AB reagent can improve the detection rate of A subtype (not including A2 subtype). By adding O cells and antibody screening cells, the detection rate of Ig M irregular antibody in blood donors was increased greatly. The clinical degenerative rate of blood in the two groups was significantly lower than that in the control group. Direct antiglobulin test detected direct anti-positive blood in blood donors. In order to avoid cross-matching the antibody screening negative but the main side of the blood agglutination phenomenon.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R446.6

【參考文獻】

相關(guān)期刊論文 前10條

1 侯玉濤;于晶晶;劉素芳;張燁;;1035例疑難交叉配血原因分析[J];國際檢驗醫(yī)學(xué)雜志;2014年19期

2 聶益軍;;惡性疾病導(dǎo)致ABO血型抗原或抗體減弱的討論[J];檢驗醫(yī)學(xué)與臨床;2014年01期

3 盛智偉;劉定文;;交叉配血不相合的原因分析及處理[J];中國保健營養(yǎng);2013年02期

4 龔晨輝;饒美英;葉水文;戴蘊;;血型不規(guī)則抗體檢測在臨床輸血中的作用[J];實驗與檢驗醫(yī)學(xué);2012年06期

5 李雪英;邱玉霞;竇茉莉;;冰凍亞型紅細胞的保存及應(yīng)用[J];山東醫(yī)藥;2012年35期

6 孫曉琳;關(guān)曉珍;于洋;陳鑫;駱群;汪德清;;36例ABO血型亞型檢測及血清學(xué)分析[J];臨床輸血與檢驗;2012年03期

7 莫秋紅;袁茜茜;周先果;劉金蓮;;Rh血型不合致新生兒溶血病的實驗結(jié)果分析及換血治療[J];國際檢驗醫(yī)學(xué)雜志;2012年13期

8 沈柏濤;謝一唯;陳秉宇;;反復(fù)輸血的受血者不完全抗體和直接抗人球蛋白試驗陽性率的研究[J];中國衛(wèi)生檢驗雜志;2012年06期

9 羅興利;李淑琴;唐潔;;微柱凝膠交叉配血不合原因分析與處理[J];檢驗醫(yī)學(xué)與臨床;2012年08期

10 曹叢雪;劉景東;;直接抗人球蛋白試驗陽性貧血患者輸血前疑難配血處理及血液成分的選擇[J];檢驗醫(yī)學(xué)與臨床;2011年24期

，

本文編號：2159560