【摘要】：目的:建立氣相色譜-質(zhì)譜聯(lián)用(GC-MS)同時(shí)測(cè)定尿液中6種內(nèi)源性類固醇激素水平的方法,對(duì)廣東省運(yùn)動(dòng)員體內(nèi)的類固醇激素代謝特征進(jìn)行統(tǒng)計(jì)歸納和比較分析,為廣東省運(yùn)動(dòng)員尿液中類固醇激素的生物護(hù)照的建立提供數(shù)據(jù)支持。方法:選取1 474名廣東省運(yùn)動(dòng)員(包括814名男隊(duì)員,660名女隊(duì)員)在賽前訓(xùn)練期間的尿樣,并對(duì)其中主要的6種內(nèi)源性類固醇進(jìn)行酶解、提取及衍生化后采用氣質(zhì)聯(lián)用(GC-MS)方法定量分析。分析采用HP-1MS色譜柱(17 m×0.2 mm,0.11μm),恒流模式,使用氦氣為載氣,流量為1 m L·min-1,進(jìn)樣量:1μL;分流比:10∶1;進(jìn)樣口溫度和EI離子源溫度分別為280℃和200℃;質(zhì)譜SCAN模式定性確證,掃描范圍m/z 40~460;選擇離子模式(SIM)下定量分析。結(jié)果:廣東省女運(yùn)動(dòng)員尿液中6種類固醇激素濃度及睪酮/表睪酮比值平均值均顯著低于國(guó)外女運(yùn)動(dòng)員(P0.05),其中睪酮濃度平均值僅為國(guó)外女運(yùn)動(dòng)員的12.2%;廣東省男運(yùn)動(dòng)員尿液中雄酮、本膽烷醇酮、睪酮、5β-雄烷-3α,17β-二醇4種類固醇激素濃度及睪酮/表睪酮比值平均值均顯著低于國(guó)外男運(yùn)動(dòng)員(P0.05),而表睪酮濃度平均值明顯高于國(guó)外男運(yùn)動(dòng)員(P0.05),5α-雄烷-3α,17β-二醇濃度平均值與國(guó)外男運(yùn)動(dòng)員基本持平(P0.05)。廣東省男、女運(yùn)動(dòng)員尿液中類固醇5α-雄烷-3α,17β-二醇/5β-雄烷-3α,17β-二醇濃度比值則均顯著高于國(guó)外男、女運(yùn)動(dòng)員的水平(P0.05),雄酮/本膽烷醇酮濃度比值與國(guó)外運(yùn)動(dòng)員基本持平。結(jié)論:本研究選取6種內(nèi)源性類固醇激素進(jìn)行氣質(zhì)聯(lián)用分析,并通過(guò)與國(guó)內(nèi)外運(yùn)動(dòng)員相關(guān)代謝指標(biāo)的對(duì)比分析,對(duì)廣東運(yùn)動(dòng)員訓(xùn)練期間的尿液中類固醇代謝特征進(jìn)行歸納,為廣東省運(yùn)動(dòng)員尿液內(nèi)源性類固醇指標(biāo)生物護(hù)照的建立提供數(shù)據(jù)支持。
[Abstract]:Objective: to establish a gas chromatography-mass spectrometry (GC-MS) method for simultaneous determination of six endogenous steroid hormones in urine, and to analyze the metabolic characteristics of steroid hormones in athletes of Guangdong province. To provide data for the establishment of biological passports of steroid hormones in the urine of athletes in Guangdong Province. Methods: urine samples were collected from 1 474 Guangdong athletes (including 814 male and 660 female players) during pre-competition training, and 6 main endogenous steroids were hydrolyzed by enzyme. After extraction and derivatization, GC-MS was used for quantitative analysis. The HP-1MS column (17m 脳 0.2mm-1 / 0.11 渭 m), constant-current mode), helium gas as carrier gas, flow rate of 1ml min-1, injection quantity of 1 渭 L, shunt ratio of 10: 1, injection temperature of 280 鈩，

本文編號(hào)：2132714