本文選題：急性心肌梗死 + miRNA��； 參考：《昆明醫(yī)科大學(xué)》2015年碩士論文

【摘要】：目的：miRNA通過調(diào)節(jié)基因的表達(dá),廣泛參與心血管系統(tǒng)細(xì)胞的增殖、遷移、分化、凋亡等病理生理過程,在心血管系統(tǒng)疾病的發(fā)生發(fā)展過程中起著重要的調(diào)控作用。本研究采用實(shí)時(shí)熒光定量PCR,檢測(cè)血漿中miRNA-30d、miRNA-199a、 miRNA-208b和miRNA-374b的表達(dá)情況,研究在STEMI、NSTEMI、UA、SA及健康對(duì)照組中的差異,探討血漿miRNA作為急性心肌梗死診斷生物學(xué)標(biāo)記物的可能性。方法：收集50位STEMI患者,50位NSTEMI患者,50位UA患者,50位SA患者及30位健康對(duì)照組血液,提取總RNA,利用實(shí)時(shí)熒光定量PCR技術(shù)檢測(cè)miRNA的表達(dá)量,并對(duì)各組間相關(guān)miRNA進(jìn)行統(tǒng)計(jì)學(xué)分析。同時(shí)檢測(cè)患者cTnI和CK-MB,分析與目的miRNA的相關(guān)性,及在診斷急性心肌梗死中的敏感度和特異性分析。結(jié)果：血漿miRNA在STEMI組、NSTEMI組、UA組、SA組中表達(dá)升高,明顯高于健康對(duì)照組,具有統(tǒng)計(jì)學(xué)意義(P0.05)。miRNA表達(dá)水平與cTnI和CK-MB呈正相關(guān)。ROC曲線顯示miRNA在診斷急性心肌梗死時(shí)明顯優(yōu)于cTnI和CK-MB,特別是胸痛發(fā)作后3小時(shí)內(nèi)。結(jié)論：實(shí)驗(yàn)結(jié)果提示心肌細(xì)胞特異性的miRNA可作為一類新的生物標(biāo)志物用于急性心肌梗死的早期診斷及治療評(píng)估。
[Abstract]:Objective to regulate the expression of genes and participate in the pathophysiological processes such as proliferation, migration, differentiation and apoptosis of cardiovascular system cells, which play an important role in the development of cardiovascular diseases. In this study, real-time fluorescent quantitative PCRs were used to detect the expression of miRNA-30dnmRNA-199ab, miRNA-208b and miRNA-374b in plasma. The differences were studied in STEMIN NSTEMIMIUASA and healthy controls, and the possibility of using plasma miRNAs as biological markers in the diagnosis of acute myocardial infarction (AMI) was investigated. Methods: 50 patients with STEMI, 50 patients with NSTEMI, 50 patients with UA, 50 patients with SA and 30 healthy controls were collected, the total RNAs were extracted and the expression of miRNA was detected by real-time fluorescence quantitative PCR. The relationship between cTnI and CK-MBs and miRNA, sensitivity and specificity in the diagnosis of acute myocardial infarction were analyzed. Results: the expression of miRNA in plasma was significantly higher in the SA group than that in the control group, and the expression of miRNA in the SA group was significantly higher than that in the healthy control group. The positive correlation between miRNA expression and cTnI and CK-MB showed that miRNA was superior to cTnI and CK-MBin in the diagnosis of acute myocardial infarction, especially within 3 hours after chest pain attack. Conclusion: the results suggest that miRNA can be used as a new biomarker for the early diagnosis and treatment of acute myocardial infarction.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R446.11

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1 李勤;血漿miRNA-30、miRNA-199、miRNA-208及miRNA-374在ACS診斷中的應(yīng)用研究[D];昆明醫(yī)科大學(xué);2015年

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