本文選題：低氧 + PIK/AKT信號通路��； 參考：《中國病理生理雜志》2016年05期

【摘要】：目的:探討低氧預處理對老年人骨髓間充質(zhì)干細胞(h BM-MSCs)的保護作用,為提高老年自體干細胞移植治療效果提供實驗支持。方法:老年h BM-MSCs于低氧培養(yǎng)箱中培養(yǎng)24 h進行低氧預處理,實驗分為年輕h BM-MSCs組(young組),老年h BM-MSCs組(old組)及低氧預處理老年h BM-MSCs組(old+hypoxia組)。300μmol/L H_2O_2作用30 min建立細胞氧化應激模型,50μmol/L LY294002作用2 h阻斷PI3K/AKT信號通路,Brd U摻入實驗檢測細胞增殖能力;CCK-8法檢測細胞活力,Western blot檢測凋亡相關(guān)蛋白Bax、Bcl-2表達水平和AKT磷酸化水平。結(jié)果:Brd U摻入實驗顯示低氧預處理的老年h BM-MSCs細胞陽性率為39.85%±3.45%,與old組相比增殖能力顯著提高(P0.05)。300μmol/L H_2O_2作用30 min誘導細胞氧化應激后,old+hypoxia組與old組比較,細胞活力顯著提高(P0.05),凋亡相關(guān)蛋白Bax表達量顯著降低(P0.05),抑制凋亡的Bcl-2蛋白表達量顯著增高(P0.05),且AKT磷酸化水平顯著增高,差異有統(tǒng)計學顯著性(P0.05);應用LY294002抑制PI3K/AKT信號通路后,細胞活力下降(P0.05)。結(jié)論:低氧預處理可以通過激活AKT信號通路提高老年人骨髓間充質(zhì)干細胞活力及增殖能力。
[Abstract]:Objective: to investigate the protective effect of hypoxic preconditioning on bone marrow mesenchymal stem cells (BM-MSCs) in the elderly and to provide experimental support for the treatment of autologous stem cell transplantation. Methods: the aged BM-MSCs was cultured in hypoxia incubator for 24 hours. The experiment was divided into three groups: young group in young BM-MSCs group, BM-MSCs group in old age group (n = 30 min) and hypoxia preconditioning group in old BM-MSCs group (n = 30 min). The model of cell oxidative stress was induced by 50 渭 mol/L LY294002 for 2 h to block the signal pathway of PI3K/AKT. The cell viability was detected by CCK-8 method. The expression of apoptosis related protein Baxfen Bcl-2 and the level of AKT phosphorylation were detected by Western blot. Results the positive rate of BM-MSCs cells was 39.85% 鹵3.45% after hypoxia preconditioning. Compared with old group, the proliferation ability of old group was significantly higher than that of old group. Compared with old group, the proliferation ability of hypoxia group was significantly higher than that of old group after 30 min induction of oxidative stress. The cell viability was increased significantly (P 0.05), the expression of apoptosis-related protein (Bax) was significantly decreased, the expression of Bcl-2 protein was significantly increased (P 0.05), and the level of AKT phosphorylation was significantly increased (P 0.05). The cell viability was decreased by P0.05. Conclusion: hypoxia preconditioning can enhance the activity and proliferation of bone marrow mesenchymal stem cells by activating AKT signaling pathway.
【作者單位】： 山西醫(yī)科大學人體解剖學教研室;山西醫(yī)科大學形態(tài)學實驗室;山西醫(yī)科大學第二醫(yī)院;
【基金】：山西醫(yī)科大學青年基金資助項目(No.02201002);山西醫(yī)科大學基礎醫(yī)學院331基金資助項目(No.201217)
【分類號】：R457.7

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