本文選題：rmtA基因 + rmtB基因��； 參考：《中華醫(yī)院感染學(xué)雜志》2016年05期

【摘要】：目的檢測質(zhì)粒介導(dǎo)的16S rRNA甲基化酶基因rmtA和rmtB,在臨床分離的產(chǎn)超廣譜β-內(nèi)酰胺酶(ESBLs)或耐阿米卡星大腸埃希菌和肺炎克雷伯菌中的分布,為臨床合理用藥提供依據(jù)。方法細菌的鑒定和藥敏試驗采用VITEK-2Compact系統(tǒng),ESBLs陽性細菌采用雙紙片協(xié)同試驗證實,采用PCR法檢測rmtA和rmtB基因。結(jié)果 108株細菌均未檢測到rmtA基因,肺炎克雷伯菌和大腸埃希菌中rmtB基因的檢出率分別為15.4%和1.8%;對于產(chǎn)ESBLs菌株,12.0%的肺炎克雷伯菌包含rmtB基因而大腸埃希菌未檢出;20.0%~62.5%的阿米卡星耐藥菌株檢測到rmtB基因,且rmtB基因陽性菌株同時對慶大霉素和妥布霉素耐藥;肺炎克雷伯菌和大腸埃希菌對阿米卡星、慶大霉素及妥布霉素的耐藥率分別為15.4%與8.9%、42.3%與62.5%、21.2%與28.6%。結(jié)論在肺炎克雷伯菌和大腸埃希菌臨床分離株存在16S rRNA甲基化酶基因,且阿米卡星耐藥菌rmtB基因陽性率相對高,所有菌株未檢出rmtA基因。
[Abstract]:Objective to detect the distribution of plasmid mediated 16s rRNA methylase genes rmtA and rmtBin in extended-spectrum 尾 -lactamases (ESBLs) or Escherichia coli and Klebsiella pneumoniae. Methods the identification of bacteria and drug sensitivity test were confirmed by VITEK-2Compact system and double disk synergy test. RmtA and rmtB genes were detected by PCR method. Results rmtA gene was not detected in 108 strains of bacteria. The detection rate of rmtB gene in Klebsiella pneumoniae and Escherichia coli were 15.4% and 1.8%, respectively. RmtB gene was detected in 12.0% of Klebsiella pneumoniae strains producing ESBLs and 62.5% in Escherichia coli. The resistance rates of Klebsiella pneumoniae and Escherichia coli to amikacin, gentamicin and tobramycin were 15.4% and 8.92.3% respectively. Conclusion 16s rRNA methylase gene exists in clinical isolates of Klebsiella pneumoniae and Escherichia coli, and the positive rate of rmtB gene in amikacin resistant bacteria is relatively high. No rmtA gene was detected in all strains.
【作者單位】： 廈門大學(xué)附屬第一醫(yī)院暨福建醫(yī)科大學(xué)教學(xué)醫(yī)院檢驗科;
【基金】：國家自然科學(xué)基金資助項目(81302529)
【分類號】：R440
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本文編號：1977469