本文選題：細(xì)胞因子誘導(dǎo)的殺傷細(xì)胞 + 抗凝劑　； 參考：《細(xì)胞與分子免疫學(xué)雜志》2016年03期

【摘要】：目的探討外周血液樣本抗凝劑類型、保存時(shí)間和保存溫度對(duì)細(xì)胞因子誘導(dǎo)的殺傷(CIK)細(xì)胞培養(yǎng)的影響,為自體免疫細(xì)胞治療技術(shù)的外周血保存標(biāo)準(zhǔn)化提供實(shí)驗(yàn)依據(jù)。方法將抽取的4份60 m L外周血樣本分別應(yīng)用肝素鈉、細(xì)胞保存液(枸櫞酸鈉)和EDTA抗凝劑抗凝,并分別存放于4、22、30℃的環(huán)境中,儲(chǔ)存0、4、8、24 h,應(yīng)用正交實(shí)驗(yàn)設(shè)計(jì)優(yōu)選為12組,然后分離出單個(gè)核細(xì)胞分別誘導(dǎo)培養(yǎng)成CIK細(xì)胞,對(duì)比12組CIK細(xì)胞增殖效率及γ干擾素(IFN-γ)分泌量的變化。結(jié)果在CIK細(xì)胞培養(yǎng)過(guò)程中,EDTA組CIK細(xì)胞增殖不明顯,肝素鈉組和血液保存液組CIK細(xì)胞增殖明顯,且肝素鈉優(yōu)于血液保存液;血液保存時(shí)間對(duì)CIK細(xì)胞培養(yǎng)影響不明顯,但保存時(shí)間越長(zhǎng),細(xì)胞增殖效率越差,保存時(shí)間超過(guò)8 h的CIK細(xì)胞,其增殖效率在第16天以后明顯降低。對(duì)培養(yǎng)時(shí)間在16 d內(nèi)的CIK細(xì)胞,血液保存溫度影響不顯著,但CIK細(xì)胞培養(yǎng)16 d之后,22℃保存條件下,CIK細(xì)胞增殖效率最高,4℃次之,30℃最差。結(jié)論肝素鈉和血液保存液均可用于CIK細(xì)胞培養(yǎng)的血液抗凝;保存時(shí)間在24 h內(nèi),溫度在4℃~30℃之間保存的血液,均可用于CIK細(xì)胞培養(yǎng)。
[Abstract]:Objective to investigate the effects of anticoagulant type, preservation time and preservation temperature on cytokine induced cytotoxicity (CIK) cell culture in peripheral blood samples, and to provide experimental evidence for the standardization of peripheral blood preservation in autologous immune cell therapy. Methods four samples of 60 mL peripheral blood were collected and treated with sodium heparin, sodium citrate (sodium citrate) and anticoagulant EDTA, respectively, and stored at 40 鈩，

本文編號(hào)：1933622