本文選題：解脲脲原體 + 多位點序列分型��； 參考：《浙江大學(xué)》2015年碩士論文

【摘要】：背景及意義 解脲脲原體(Ureaplasma, UU)屬柔膜綱支原體目支原體科脲原體屬,是一種介于細(xì)菌與病毒之間的一種能獨立生活的最小原核細(xì)胞型微生物,它與微小脲原體、生殖脲原體等常常寄居在正常人群生殖道、泌尿道。但也是一種易引起生殖道、泌尿道感染的病原微生物,有大量文獻報道,它們會引起男性的前列腺炎、尿道炎,尿結(jié)石,腎盂腎炎等,對于女性,還常常是陰道炎、子宮頸炎、盆腔炎、生殖腔道的炎性反應(yīng)、粘連與阻塞,以及造成不孕不育、流產(chǎn)的病原體之一。曾經(jīng)我們認(rèn)為,UU引起的感染是否發(fā)生很大程度上取決于人體的抵抗屏障和免疫狀態(tài)是否正常；但近來有很多文獻發(fā)現(xiàn),UU有不同的生物群和亞群,而是否引起感染貌似和他們也有著很大的聯(lián)系。 研究者們已建立了多種針對UU的基因分型方法,包括傳統(tǒng)的聚合酶鏈反應(yīng)(polymerase chain reaction, PCR),限制性片段長度多態(tài)性(restriction fragment length polymorphism, RFLP)等。然而,針對UU特定血清型或亞型與某種特定疾病的關(guān)聯(lián)還未見報道。為了鑒定具有引起特定疾病的UU亞群并研究UU分子流行病學(xué)信息,迫切需求新的高分辨率分型方法的建立。 本次研究利用基于4個管家基因(ftsH, rpL22, valS, thrS)進行的多位點序列分型(MLST)方法對283株解脲脲原體進行分析,其中包括14種血清型標(biāo)準(zhǔn)菌株及臨床樣本分離得到的269株解脲脲原體。結(jié)果發(fā)現(xiàn)MLST序列能夠在探索脲原體屬分子流行病學(xué)及人群構(gòu)成方面有很高的鑒別能力。 目的 研究解脲脲原體臨床菌株的分子流行病學(xué)規(guī)律和種群結(jié)構(gòu),明確遺傳背景,為解脲脲原體的感染診治提供理論參考。 方法 269株解脲脲原體臨床菌株,其中134株來源于有癥狀患者,135株來源于無癥狀人群,與14株標(biāo)準(zhǔn)菌株開展多位點序列分型(MLST)研究,并應(yīng)用MEGA5與START2軟件進行生物信息學(xué)分析,以揭示其流行趨勢。 結(jié)果 分析283株解脲脲原體發(fā)現(xiàn)可分成99種序列型(STs),14種血清型標(biāo)準(zhǔn)菌株分為12種STs,269株臨床菌株可分為87種特異性STs。ST1和ST22為主要的STs,分別占有68和70株。eBURST分析表明存在兩個克隆復(fù)合體(CC1和CC2),并且通過標(biāo)準(zhǔn)連鎖指數(shù)(IAS)分析顯示存在連鎖不平衡。系統(tǒng)發(fā)育學(xué)分析顯示解脲脲原體14種血清型標(biāo)準(zhǔn)菌株分為兩個種群,此結(jié)果與解脲脲原體的物種分群(分為解脲脲原體和微小脲原體兩類)高度相符。對269株臨床菌株進行生物群分析,CC1中的菌株都是微小脲原體,CC2中的是解脲脲原體,此外,陰道炎、輸卵管阻塞及子宮頸炎患者中CC2型感染更為多見。 結(jié)論 解脲脲原體MLST方案具有很高的分辨率,適用于解脲脲原體的分子流行病學(xué)和種群結(jié)構(gòu)分析。
[Abstract]:Background and significance Ureaplasma Ureaplasma (UU) belongs to the genus Ureaplasma of Mycoplasma Mycoplasma. Ureaplasma Urealyticum (UU) is a minimal prokaryotic microorganism that can live independently between bacteria and viruses. Reproductive Ureaplasma and so on often sojourn in the normal population genital tract, urinary tract. However, it is also a pathogenic microorganism that can easily cause genital tract, urinary tract infection, which has been reported in a large number of documents. They can cause prostatitis, urethritis, urinary calculi, pyelonephritis and so on in men. For women, they are often vaginitis, cervicitis, etc. Pelvic inflammation, inflammation of the reproductive tract, adhesion and obstruction, and one of the pathogens causing infertility and abortion. We used to think that infection caused by UU depends largely on whether the body's resistance barrier and immune state are normal, but there have been a lot of literature recently found that the UU has different biota and subgroups. Whether or not to cause infection seems to have a strong connection with them. Many genotyping methods for UU have been established, including polymerase chain reactionation (PCR), restriction fragment length polymorphism, RFLP) restriction (restriction fragment length polymorphism, RFLP), and so on. However, the association of UU specific serotypes or subtypes with a specific disease has not been reported. In order to identify the UU subgroups that cause specific diseases and to study the molecular epidemiological information of UU, it is urgent to establish a new high resolution typing method. In this study, 283 strains of Ureaplasma Urealyticum were analyzed by multilocus sequence typing based on four housekeeping genes, ftsH, rpL22, valSST, and 269 strains of Ureaplasma Urealyticum were isolated from 14 serotype standard strains and clinical samples. The results showed that MLST sequence could be used to identify the molecular epidemiology and population composition of Ureaplasma. Purpose To study the molecular epidemiology and population structure of Ureaplasma Urealyticum clinical strains, to clarify the genetic background, and to provide a theoretical reference for the diagnosis and treatment of Ureaplasma Urealyticum infection. Method There were 269 clinical strains of Ureaplasma Urealyticum, of which 134 were from asymptomatic patients and 135 were from asymptomatic population. Multilocus sequence typing was carried out with 14 standard strains, and bioinformatics analysis was carried out with MEGA5 and START2 software. To reveal its trend. Result The analysis of 283 strains of Ureaplasma Urealyticum can be divided into 99 serotypic strains and 14 serotypic standard strains, which can be divided into 12 strains of STS, which can be divided into 87 kinds of specific STs.ST1 and 70 strains of STS. EBURST analysis shows that there are two kinds of STS. The analysis of CC1 and CC2 by the standard linkage index (IASA) showed linkage disequilibrium. Phylogenetic analysis showed that 14 strains of Ureaplasma Urealyticum serotype were divided into two populations, which was highly consistent with the species groups of Ureaplasma Urealyticum and Ureaplasma minimus. Biota analysis of 269 clinical strains of CC1 was carried out. Ureaplasma Urealyticum was found in CC2. In addition, CC2 type infection was more common in patients with vaginitis, tubal obstruction and cervicitis. Conclusion Ureaplasma Urealyticum MLST has high resolution and is suitable for molecular epidemiology and population structure analysis of Ureaplasma Urealyticum.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R446.5

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