本文選題：耐甲氧西林金黃色葡萄球菌 + 葡萄球菌染色體mec盒��； 參考：《中華醫(yī)院感染學(xué)雜志》2016年03期

【摘要】：目的檢測(cè)臨床分離的耐甲氧西林金黃色葡萄球菌(MRSA)葡萄球菌染色體mec盒(SCCmec)基因型并根據(jù)分型結(jié)果分析耐藥性,為臨床治療MRSA感染提供參考依據(jù)。方法收集2013年8月-2014年5月臨床分離的金黃色葡萄球菌122株,采用VITEK-2全自動(dòng)微生物分析儀完成藥敏鑒定,普通PCR技術(shù)檢測(cè)mecA基因,并采用多重PCR對(duì)其進(jìn)行SCCmec基因型分型。結(jié)果 122株金黃色葡萄球菌,經(jīng)mecA基因篩選后,檢出MRSA 47株,檢出率38.5%,SCCmec分型結(jié)果表明,SCCmecⅢ型菌株35株占74.5%,SCCmecⅡ型菌株6株占12.8%,SCCmecV型2株占4.3%,未分型菌株4株占8.5%;SCCmecⅡ型、SCCmecⅢ型除了對(duì)β-內(nèi)酰胺類藥物廣泛耐藥外,還對(duì)喹諾酮類、大環(huán)內(nèi)酯類、克林霉素、四環(huán)素具有較高的抗藥活性,而SCCmecV型僅對(duì)紅霉素、克林霉素、四環(huán)素耐藥;所有菌株均對(duì)呋喃妥因、萬古霉素、替加環(huán)素、利奈唑胺穩(wěn)定敏感。結(jié)論醫(yī)院臨床分離的MRSA以SCCmecⅢ型為主,其次為SCCmecⅡ型,并有少量SCCmecV型出現(xiàn);SCCmecⅢ型和SCCmecⅡ型對(duì)抗菌藥物均呈多藥耐藥性。
[Abstract]:Objective to detect the genotypes of methicillin-resistant Staphylococcus aureus (MRSA) chromosome mec cassette (SCC mecc) and to analyze the drug resistance according to the results of the genotyping so as to provide a reference for clinical treatment of MRSA infection. Methods 122 strains of Staphylococcus aureus isolated from August 2013 to May 2014 were collected. The drug sensitivity was identified by VITEK-2 automatic microbiological analyzer. The mecA gene was detected by ordinary PCR technique. The SCCmec genotypes were genotyped by multiple PCR. Results among the 122 strains of Staphylococcus aureus, 47 strains of MRSA were detected by mecA gene screening. The results of SCCmec typing showed that 35 strains of SCCmec 鈪，

本文編號(hào)：1916005