發(fā)布時(shí)間：2018-04-10 06:25

  本文選題：金黃色葡萄球菌　切入點(diǎn)：MRSA　出處：《現(xiàn)代預(yù)防醫(yī)學(xué)》2016年23期

【摘要】：目的分析臨床分離金黃色葡萄球菌(Staphylococcus aureus,S.aureus)中苯唑西林敏感mec A基因陽性的金黃色葡萄球菌(OS-MRSA)的發(fā)生率和生物學(xué)特性,為臨床預(yù)防和治療S.aureus感染提供理論依據(jù)。方法臨床分離的872株S.aureus經(jīng)全自動(dòng)微生物分析儀鑒定及藥物敏感性檢測,表型分析判定苯唑西林耐藥金黃色葡萄球菌(MRSA)菌株和苯唑西林敏感金黃色葡萄球菌(MSSA)菌株;采用聚合酶鏈?zhǔn)椒磻?yīng)(PCR)檢測mec A基因和其他金黃色葡萄球菌特異基因,通過對比表型和基因分析結(jié)果鑒定出OS-MRSA。結(jié)果 872株S.aureus根據(jù)臨床表型分析判定610株苯唑西林耐藥(MRSA)菌株,262株苯唑西林敏感(MSSA)菌株,篩選鑒定出5株OS-MRSA,共4種基因類型,其中有兩株屬于同一基因類型。結(jié)論該院兩年內(nèi)臨床S.aureus感染中根據(jù)表型分析MRSA占70%,MSSA占30%,而OSMRSA占"MSSA"的2%。
[Abstract]:Objective to analyze the incidence and biological characteristics of oxacillin sensitive mec A gene positive staphylococcus aureus OS-MRSAs isolated from Staphylococcus aureus S.aureus in order to provide a theoretical basis for clinical prevention and treatment of S.aureus infection.Methods A total of 872 clinical isolates of S.aureus were identified by automatic microbiological analyzer and drug sensitivity test. Phenotypic analysis showed that the strains of oxacillin resistant Staphylococcus aureus and oxacillin sensitive Staphylococcus aureus were identified by phenotypic analysis.Mec A gene and other Staphylococcus aureus specific genes were detected by polymerase chain reaction (PCR), and OS-MRSAs were identified by comparative phenotype and gene analysis.Results according to the clinical phenotypic analysis, 872 S.aureus strains were identified as oxacillin resistant strains and 262 oxacillin sensitive strains. Five OS-MRSAs were identified, and two of them belonged to the same gene type.Conclusion according to phenotypic analysis, MRSA accounted for 30% of clinical S.aureus infection and OSMRSA accounted for 2% of "MSSA" in this hospital within two years.
【作者單位】： 安徽中醫(yī)藥大學(xué)第一附屬醫(yī)院檢驗(yàn)中心;
【分類號】：R446.5

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