本文選題：微流控　切入點：超聲封合　出處：《大連理工大學(xué)》2015年碩士論文

【摘要】：即時檢測(POCT)技術(shù)是一種臨床醫(yī)學(xué)中的快速檢測技術(shù),目前已廣泛應(yīng)用于血糖、腫瘤檢測等各個方面。基于微流控的即時檢測芯片屬于微流控技術(shù)、生物醫(yī)學(xué)工程、先進制造技術(shù)等的聯(lián)合應(yīng)用,本文圍繞該芯片的研制,做了以下幾個方面的研究：確定了基于微流控技術(shù)、熒光免疫檢測技術(shù)的即時檢測芯片方案。芯片的材料采用熱塑性聚合物,結(jié)合注塑成型工藝和超聲波封合技術(shù),以實現(xiàn)芯片批量制造的目的。設(shè)計的芯片結(jié)構(gòu)主要包括進樣區(qū)、混合區(qū)、流速控制結(jié)構(gòu)、檢測區(qū)和廢液回收區(qū)等。為驗證芯片結(jié)構(gòu)方案的可行性,采用微加工工藝,制作出SU-8模具,并采用澆注的方法,制作出PDMS微流控芯片,對芯片各功能區(qū)域特別是流速控制結(jié)構(gòu),進行功能性試驗,最終確定了芯片設(shè)計方案。選用超聲波封合作為芯片的封合方法,并設(shè)計了一種配合式的導(dǎo)能筋結(jié)構(gòu)。合理設(shè)計了芯片的整體結(jié)構(gòu),使其有利于注塑成型的質(zhì)量,以及符合芯片的定位和封合過程中的裝夾要求�；赥aguchi正交試驗設(shè)計方法,進行芯片的注塑成型工藝試驗,獲得了各工藝參數(shù)對芯片注塑成型質(zhì)量的主次影響順序和其最優(yōu)水平組合,得出了各個注塑成型工藝參數(shù)對芯片微結(jié)構(gòu)填充和封合面的平面度的影響規(guī)律；通過訂制特定的焊頭,自行設(shè)計和制作芯片夾具,搭建超聲波封合實驗平臺。通過多次封合實驗,得到超聲封合最佳工作參數(shù)。以羊全血作為實驗樣品,進行芯片的自進樣實驗。實驗過程中,芯片各區(qū)域都表現(xiàn)出較好的流動穩(wěn)定性,通過15組重復(fù)實驗得出,血樣流動前沿流過流速控制結(jié)構(gòu)的時間的標(biāo)準(zhǔn)差為8.3s,流過整個芯片的時間的標(biāo)準(zhǔn)差為19.4s。
[Abstract]:Instant detection technique is a rapid detection technique in clinical medicine, which has been widely used in blood sugar, tumor detection and so on.The real-time detection chip based on microfluidic is the joint application of microfluidic technology, biomedical engineering, advanced manufacturing technology and so on.The real-time detection chip scheme of fluorescence immunoassay technology.The chip is made of thermoplastic polymer, injection molding technology and ultrasonic sealing technology.The chip structure includes sampling area, mixing area, flow control structure, detection area and waste recovery area.In order to verify the feasibility of the chip structure, the SU-8 mould was fabricated by micromachining technology, and the PDMS microfluidic chip was fabricated by pouring method. The functional tests were carried out on the functional regions of the chip, especially the flow control structure.Finally, the chip design scheme is determined.The ultrasonic sealing cooperation is selected as the sealing method of the chip, and a kind of matching energy conduction bar structure is designed.The whole structure of the chip is designed reasonably, which is propitious to the quality of injection molding, and meets the requirements of chip positioning and clamping in the process of sealing.Based on the orthogonal design method of Taguchi, the injection molding process of the chip was tested, and the sequence of the main and secondary effects of each process parameter on the injection molding quality of the chip and its optimal level combination were obtained.The effects of various injection molding process parameters on the flatness of the microstructural filling and sealing surfaces of the chip were obtained. The ultrasonic sealing experimental platform was built by customizing the specific welding head and designing and making the chip clamp by itself.The optimum working parameters of ultrasonic sealing were obtained through several sealing experiments.The whole blood of sheep was used as the experimental sample, and the self-injection experiment of the chip was carried out.In the process of the experiment, the flow stability of each region of the chip is good. Through 15 groups of repeated experiments, the standard deviation of the time of the blood flow front flowing through the flow velocity control structure is 8.3 s, and the standard deviation of the time passing through the whole chip is 19.4s.
【學(xué)位授予單位】：大連理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R446;TN492

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本文編號：1711390