本文關(guān)鍵詞： 多藥耐藥 鮑氏不動桿菌 生物膜 葡萄糖　出處：《中華醫(yī)院感染學(xué)雜志》2016年03期 　論文類型：期刊論文

【摘要】：目的探索多藥耐藥鮑氏不動桿菌在不同濃度葡萄糖環(huán)境中其生物膜形成能力的變化,為糖尿病患者預(yù)防與治療感染提供理論依據(jù)。方法用測定MIC方法篩選2012年4月-2013年10月臨床分離的多藥耐藥鮑氏不動桿菌,用含有不同葡萄糖濃度的胰蛋白胨大豆肉湯培養(yǎng),分別測定其570nm處吸光度值,采用激光共聚焦顯微鏡(CLSM)觀察和測定生物膜在不同濃度、不同時(shí)間形成的形態(tài)及厚度,不同濃度下膜生成能力采用Kruskal-Wallis H檢驗(yàn)及t檢驗(yàn)法進(jìn)行統(tǒng)計(jì)。結(jié)果 30株多藥耐藥鮑氏不動桿菌對頭孢他啶、哌拉西林、阿米卡星的耐藥率均為96.7%;葡萄糖濃度為11.1~30.0mmol/L時(shí)膜形成能力與無糖培養(yǎng)比較吸光度值差異有統(tǒng)計(jì)學(xué)意義(P0.05),但≥11.1mmol/L的各濃度吸光度值比較差異無統(tǒng)計(jì)學(xué)意義;CLSM觀察顯示,多藥耐藥鮑氏不動桿菌葡萄糖濃度在11.1~30.0mmol/L時(shí)菌株熒光強(qiáng)度均在大約24h基本達(dá)到最強(qiáng),48h有一個(gè)強(qiáng)度維持,比無糖環(huán)境提前24h;生物膜厚度數(shù)據(jù)統(tǒng)計(jì)顯示,加糖培養(yǎng)12~36h膜厚度達(dá)到最大,加糖培養(yǎng)膜厚度最大值與無糖培養(yǎng)膜厚度最大值相比差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論多藥耐藥鮑氏不動桿菌在環(huán)境中葡萄糖濃度≥11.1mmol/L時(shí),形成生物膜的能力明顯增強(qiáng),形成生物膜的厚度增加,形成生物膜的周期明顯縮短。
[Abstract]:Objective to explore the change of biofilm formation ability of Acinetobacter baumannii in different glucose concentration. Methods the multidrug resistant Acinetobacter baumannii isolated from April 2012 to October 2013 was screened by MIC assay and cultured in soybean broth containing different glucose concentrations. The absorbance values at 570 nm were measured, and the morphology and thickness of biofilm formed at different concentration and time were observed and measured by laser confocal microscope (CLSM). Results 30 strains of multidrug resistant Acinetobacter baumannii were treated with ceftazidime and piperacillin. The drug resistance rates of amikacin were 96.7mg / L, and the membrane forming ability was significantly different from that of sugar-free culture when glucose concentration was 11.1 渭 mol / L (30.0 mmol / L), but there was no significant difference between the absorbance values of each concentration 鈮，

本文編號：1550749