本文關(guān)鍵詞： 酶聯(lián)免疫試驗(yàn) 核酸擴(kuò)增技術(shù) 檢測模式　出處：《中國輸血雜志》2016年07期 　論文類型：期刊論文

【摘要】：目的比較核酸檢測技術(shù)(NAT)在無償獻(xiàn)血不同血液安全篩查模式下陽性反應(yīng)率。方法在2010年1月1日-2013年8月31日期間,采用2遍ELISA檢測,無反應(yīng)性樣本采用Cobas Taqsceen MPX Test進(jìn)行1遍NAT;2013年9月1日-2014年9月29日期間,采用1遍ELISA檢測,無反應(yīng)性樣本采用Cobas Taqsceen MPX Test進(jìn)行1遍NAT;2014年9月29日-2015年8月31日期間,采用1遍ELISA檢測,無反應(yīng)性樣本采用Cobas Taqsceen MPX Test v2.0(MPX v2.0)進(jìn)行1遍NAT。對NAT混檢陽性pool采用MPX v2.0拆分單檢,并鑒別病毒種類;對ELISA檢測抗-HIV無反應(yīng)性、HIV RNA有反應(yīng)性的獻(xiàn)血者定期進(jìn)行追蹤分析,觀察有無血清學(xué)轉(zhuǎn)換,以確定感染狀態(tài)。結(jié)果3個階段共完成NAT標(biāo)本422 667份,混檢陽性898pools,陽性率0.21%,其中HBVDNA混檢陽性率為0.209%(893/422 667),HCVRNA混檢陽性1例(1/422 667),HIVRNA混檢陽性4例(4/422 667);拆分單檢總陽性率為0.126%(551/422 667),拆分陽性率為61.35%(551/898),拆分后單檢HBVDNA陽性545例,HCVRNA陽性2例,HIVRNA陽性4例。對HIVRNA陽性樣本進(jìn)行定期追蹤分析,4例獻(xiàn)血者均在獻(xiàn)血后3個月內(nèi)發(fā)生血清學(xué)陽轉(zhuǎn),確定均為窗口期感染HIV。3個階段采用不同的檢測模式,NAT總陽性反應(yīng)率無顯著性差異;拆分單檢陽性反應(yīng)率Ⅲ階段高于Ⅰ階段(P0.05)。結(jié)論 NAT檢測的總陽性反應(yīng)率與檢測模式無關(guān);應(yīng)用NAT可降低輸血風(fēng)險,尤其對HBV窗口期感染的陽性檢出率較高。
[Abstract]:Objective to compare the positive reaction rate of nucleic acid test (Nat) in different blood safety screening modes of blood donation. Methods from January 1st 2010 to August 31st 2013, ELISA was used twice. The non-reactive samples were performed once with Cobas Taqsceen MPX Test; during the period from September 1st 2013 to September 29th 2014, with one ELISA test; with Cobas Taqsceen MPX Test; and with Cobas Taqsceen MPX Test with the period September 29th 2014 to September 29th 2014. ELISA was used once, and Cobas Taqsceen MPX Test v2.0MPX v2.0 was used to detect the non-reactive samples. MPX v2.0 was used to separate the NAT positive pool and identify the virus types. In order to determine the status of infection, the blood donors who were tested by ELISA for anti-HIV / RNA reactivity were regularly followed up and analyzed. Results 422,667 NAT samples were collected in three stages. The positive rate of HBVDNA mixed detection was 0.209 and 0.209 respectively. One case was positive for 1 / 422 667HIV RNA, 4 cases were positive for HIV RNA, the total positive rate was 0.1266 551 / 422 667G, the positive rate of HBVDNA was 61.35% 551% 8987.After resolution, 545 cases were positive for HBVDNA and 545 cases were positive for HIV RNA and HBVDNA respectively, respectively. The total positive rate was 0.1266 551 / 422 667G, and the positive rate of HBVDNA was 61.35% 551% 8988.The positive rate of HBVDNA was 551% 8988.The positive rate of HBVDNA was 545 cases after the resolution, and the total positive rate was 0.1266 551 / 422,667%. The total positive rate of HBVDNA was 0.126 551% 898%. 4 cases were positive. The positive samples of HIVRNA were regularly followed up. 4 cases of blood donors all developed serological positive conversion within 3 months after blood donation. There was no significant difference in the total positive reaction rate of Nat in all the stages of window infection with different detection modes, and the positive reaction rate in stage 鈪，

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