本文關(guān)鍵詞： 瘰疬分枝桿菌 real-time PCR 非結(jié)核分枝桿菌　出處：《中國人獸共患病學(xué)報(bào)》2016年12期 　論文類型：期刊論文

【摘要】：目的建立針對(duì)非結(jié)核分枝桿菌中瘰疬分枝桿菌菌株的real-time PCR檢測方法。方法在我們的研究中,根據(jù)瘰疬分枝桿菌的SodA基因設(shè)計(jì)MGB探針和特定的引物,并用來檢測模擬樣品中的瘰疬分枝桿菌。結(jié)果該方法最低瘰疬分枝桿菌DNA檢測濃度是101拷貝數(shù),標(biāo)準(zhǔn)曲線顯示閾值周期和SodA基因片段拷貝數(shù)之間的相關(guān)系數(shù)是0.973,斜率為-3.249,表現(xiàn)出良好的線性關(guān)系。此外,模擬樣品最低檢測濃度是每毫升101個(gè)細(xì)菌,此外,其他9株菌為陰性結(jié)果,驗(yàn)證了良好的特異性。結(jié)論該方法對(duì)于檢測瘰疬分枝桿菌模擬標(biāo)本顯示很高的敏感性和特異性,可用于瘰疬分枝桿菌菌株的生態(tài)和流行病學(xué)監(jiān)測。
[Abstract]:Objective to establish a real-time PCR method for the detection of Mycobacterium scrofulla in non-tuberculous mycobacteria. MGB probes and specific primers were designed according to the SodA gene of Mycobacterium scrofulla. The method was used to detect Mycobacterium scrofula in simulated samples. Results the lowest DNA detection concentration of Mycobacterium scrofula was 101-copy number. The standard curve showed that the correlation coefficient between threshold period and copy number of SodA gene fragment was 0.973, slope was -3.249, showing a good linear relationship. The minimum detectable concentration of simulated samples was 101 bacteria per milliliter, in addition, the other 9 strains were negative. Conclusion this method is highly sensitive and specific for the detection of mimic specimens of Mycobacterium scrofula, and can be used for ecological and epidemiological surveillance of Mycobacterium scrofula.
【作者單位】： 中國疾病預(yù)防控制中心傳染病預(yù)防控制所 傳染病預(yù)防控制國家重點(diǎn)實(shí)驗(yàn)室 感染性疾病診治協(xié)同創(chuàng)新中心;溫州醫(yī)科大學(xué)檢驗(yàn)醫(yī)學(xué)院生命科學(xué)學(xué)院;
【基金】：financially supported by the projects 81401647 of Natural Science Foundation of China 2013ZX10003006 and 2013ZX10003002001 of Chinese National Key Program of Mega Infectious Diseases~~
【分類號(hào)】：R440
【正文快照】： IntroductionM.scrofulaceumis　a　slow-growing　environ-Mycobacteria　that　are　not　members　of　the　M.mental　and　opportunistic　atypical　mycobacteriumtuberculosis　complex(M.africanum,M.bovis,that　causes　cervical　lymphadenopathy　in　childrenM.b，

本文編號(hào)：1452543