本文關(guān)鍵詞：雙抗體夾心ELISA法檢測石頭魚毒素　出處：《軍事醫(yī)學》2016年06期 　論文類型：期刊論文

  更多相關(guān)文章： 石頭魚毒素 NeoVTX 酶聯(lián)免疫吸附試驗 雙抗體夾心法

【摘要】：目的建立石頭魚毒素neoVTX的酶聯(lián)免疫吸附試驗(ELISA)檢測方法,為該毒素的檢測、中毒診斷及治療提供依據(jù)。方法以過碘酸鈉法偶聯(lián)馬抗-HRP,利用雙抗體夾心ELISA方法檢測neoVTX。結(jié)果該法的線性范圍在磷酸鹽緩沖液(PBS)中為4~512 ng/ml,線性回歸方程為Y=1.676X-1.140(r~2=0.9728,n=8,P0.0001),在血漿中檢測線性范圍為4~2048 ng/ml,線性回歸方程為Y=0.3094X-0.1208(r~2=0.9907,n=10,P0.0001)。牛血清白蛋白、卵清蛋白及人血漿對檢測結(jié)果無干擾。結(jié)論成功建立了石頭魚毒素的雙抗體夾心ELISA檢測方法,該法有較高的靈敏度和特異性,適用于溶液中及血漿中微量neoVTX的樣品分析。
[Abstract]:Objective to establish an enzyme linked immunosorbent assay (Elisa) for the detection of stonefish toxin neoVTX. Methods the method of sodium periodate combined with horse anti-HRP was used to provide evidence for the diagnosis and treatment of poisoning. A double antibody sandwich ELISA method was used to detect neoVTX.Results the linear range of this method was 4 512 ng/ml in phosphate buffer solution. The linear regression equation was YT 1.676X-1.140C 0.9728NM (P0.0001A), and the linear range of detection in plasma was 4m 2048 ng/ml. The linear regression equation was YT 0.3094 X-0.1208 ru 20.9907 ~ (10) P0.00010.Bovine Serum Albumin (BSA). Ovalbumin and human plasma do not interfere with the detection results. Conclusion A double antibody sandwich ELISA method for the detection of stonefish toxin has been successfully established. The method has high sensitivity and specificity. It is suitable for sample analysis of trace neoVTX in solution and plasma.
【作者單位】： 軍事醫(yī)學科學院生物工程研究所;
【基金】：國家863計劃資助項目(SS2013AA090204)
【分類號】：R446.61
【正文快照】： 石頭魚(Synanceia)屬輻鰭類亞綱(Actinoptery-gii)湁形目(Scorpaeniformes)毒湁科(Synanceidae),主要存在于太平洋和印度洋的熱帶水域,被認為是毒性最強的魚類之一[1]。其背部的毒刺引起的刺傷,可引起劇痛、低血壓、水腫、惡心、頭暈、胸悶、肌無力以及呼吸和循環(huán)衰竭,甚至死

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1 張韞;陳亞利;宋詩雨;王森;王e，

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