本文關(guān)鍵詞：幽門螺桿菌感染對胃黏膜NADPH氧化酶DUOX2和NOX2表達的影響　出處：《河南科技大學》2015年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 幽門螺桿菌 DUOX2 NOX2 胃黏膜 Cag A Vac A

【摘要】：目的:研究幽門螺桿菌(Helicobacter pylori,Hp)感染對胃黏膜DUOX2和NOX2基因和蛋白表達的影響,進一步揭示胃疾病的發(fā)病機理。方法:收集2013年8月至2014年2月有上消化道癥狀到河南科技大學第一附屬醫(yī)院做胃鏡檢查的患者胃黏膜組織標本200例,其中男性113例,女性87例,年齡16-86歲,平均52.3歲。采用快速尿素酶試驗檢測Hp感染情況;酶聯(lián)免疫吸附實驗檢測Hp陽性患者血清中Cag A和Vac A抗體的表達情況;RT-PCR檢測胃黏膜DUOX2和NOX2 m RNA的表達情況;免疫組化和Western Blot檢測胃黏膜DUOX2和NOX2蛋白的表達情況。結(jié)果:1.快速尿素酶試驗結(jié)果顯示Hp陽性者111例(男69例,女42例),Hp陰性者89例(男44例,女45例)。2.RT-PCR結(jié)果顯示DUOX2 m RNA在Hp陽性患者胃黏膜組織中的表達明顯低于Hp陰性患者(P=0.02);Hp毒力強(Cag A+/Vac A+)的菌株胃黏膜DUOX2 m RNA表達量低,Hp毒力弱(Cag A-/Vac A-)的菌株胃黏膜DUOX2m RNA表達量高(均P0.05)。胃黏膜NOX2 m RNA水平在Hp(+)組的表達量高于Hp(-)組(P0.05),但在感染不同毒力的Hp的組別之間NOX2m RNA表達無明顯差別。3.免疫組化結(jié)果顯示DUOX2蛋白在200例胃黏膜組織中均有表達,與基因水平上的表達一致。DUOX2蛋白在Hp陽性組患者胃黏膜表達量明顯低于Hp陰性組(P0.05);在Hp陽性的胃黏膜組織中與Cag A-/Vac A-組相比,Cag A+或Vac A+組DUOX2蛋白表達降低,Cag A+/Vac A+組DUOX2蛋白表達最低(均P0.05)。NOX2表達于胃黏膜炎性細胞內(nèi),Hp(+)組NOX2蛋白的表達較Hp(-)組升高(P0.05);在感染不同毒力的Hp的組別之間NOX2蛋白表達無差別。4.Western Blot結(jié)果顯示胃黏膜組織DUOX2蛋白在Hp(+)組的表達量低于Hp(-)組,后者是前者的2.2倍。結(jié)論:1.Hp感染后胃黏膜DUOX2表達降低,NOX2表達升高,提示Hp感染對胃黏膜DUOX2和NOX2的表達均具有一定的影響作用。2.Hp感染后抑制了DUOX2的表達,且毒力越強的菌株對DUOX2表達的抑制作用越強;相反,Hp的感染促進了胃黏膜NOX2的表達,但NOX2表達量的多少與Hp的毒力無關(guān)。3.胃黏膜DUOX2和NOX2在Hp感染所致胃疾病的發(fā)生發(fā)展中起到一定的作用。
[Abstract]:Objective: To study the effect of Helicobacter pylori (Helicobacter pylori, Hp) infection on the expression of DUOX2 in gastric mucosa and NOX2 gene and protein, further reveal the pathogenesis of gastric diseases. Methods: from August 2013 to February 2014 with upper gastrointestinal symptoms in Henan University of Science and Technology First Affiliated Hospital patients for gastroscopy gastric mucosa specimens from 200 patients, among them 113 cases were male, 87 were female, aged 16-86 years old, average 52.3 years old. By rapid urease test to detect Hp infection; the expression of enzyme-linked immunosorbent assay in patients with positive serum Hp Cag A and Vac A antibody; expression of RT-PCR DUOX2 and NOX2 M detection of gastric mucosa RNA expression; immunohistochemistry Western and Blot detection of DUOX2 in gastric mucosa and NOX2 protein. Results: 1. rapid urease test results showed that 111 cases were Hp positive (male 69 cases, female 42 cases), 89 cases of Hp negative patients (male 44 cases, female 45 cases).2.RT -PCR results showed that the expression of DUOX2 m RNA in Hp positive patients with gastric mucosa was significantly lower than that of Hp negative patients (P=0.02); Hp (Cag A+/Vac A+) virulent strain DUOX2 in gastric mucosa of M RNA low expression, Hp (Cag A-/Vac A-) weak virulent strains of DUOX2m in gastric mucosa of RNA high expression (P0.05 NOX2 m). Gastric mucosal RNA levels in Hp (+) expression group was higher than that of Hp (-) group (P0.05), but in between the infection of different Hp strains group NOX2m RNA expression had no significant difference.3. immunohistochemistry showed that DUOX2 protein in 200 cases of gastric mucosa tissues. The expression of.DUOX2 protein the expression of Hp in gastric mucosa of patients with positive group were significantly lower than that in Hp negative group and gene expression level of (P0.05); in Hp positive gastric mucosa compared with Cag A-/Vac group A-, Cag A+ or Vac A+ reduced the expression of DUOX2 protein in Cag group, A+/Vac A+ group of DUOX2 protein expression in the lowest expression of.NOX2 (P0.05) In the gastric mucosa inflammatory cells, Hp (+) NOX2 protein expression group than in Hp (-) group (P0.05); infection between different Hp strains group NOX2 protein expression.4.Western Blot showed no difference in gastric mucosa of DUOX2 protein in Hp (+) expression was lower than that in Hp (- group), which is 2.2 times the former. Conclusion: 1.Hp infection in gastric mucosa after DUOX2 expression decreased, NOX2 expression increased, suggesting that Hp infection on the expression of DUOX2 in gastric mucosa and NOX2 have certain influence after.2.Hp infection inhibited the expression of DUOX2, the stronger the inhibition and the more virulent strain on the expression of DUOX2 on the contrary, Hp infection; promote the expression of NOX2 in gastric mucosa, but the expression of NOX2 and the amount of Hp is independent of.3. DUOX2 in gastric mucosa and toxicity of NOX2 in Hp induced gastric diseases play a certain role in the development.

【學位授予單位】：河南科技大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R446.6

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本文編號：1425294