本文關鍵詞：質(zhì)譜分析儀對耐甲氧西林金黃色葡萄球菌分子分型的應用研究　出處：《中華醫(yī)院感染學雜志》2016年04期 　論文類型：期刊論文

  更多相關文章： 質(zhì)普分析儀 基質(zhì)輔助激光解析電離飛行時間質(zhì)譜 耐甲氧西林金黃色葡萄球菌 ClinProTools軟件 多位點序列分析

【摘要】：目的評價基質(zhì)輔助激光解析電離飛行時間質(zhì)譜技術(MALDI-TOF MS)對耐甲氧西林金黃色葡萄球菌(MRSA)分子分型的應用價值。方法收集2009年1月-2012年3月來自上海、重慶、廣州、沈陽、烏魯木齊、北京6個城市共270株MRSA,經(jīng)多位點序列分析(MLST)表型主要為St239、St5、St59、St45 4個基因型,使用ClinProTools軟件建立模型,并用剩余菌株驗證得出模型的敏感性和特異性;隨機選取試驗組35株St239,對照組43株包括20株St5、15株St59、8株St45構建模型St239;隨機選取試驗組8株St45,對照組35株包括11株St5、8株St59、16株St239構建模型St45。結(jié)果 154株St239、72株St5、30株St59、14株St45經(jīng)檢測顯示,模型St239、St45、St5的交叉效度和可接受度均為100.0%,僅模型St59的交叉效度為90.5%,其可接受度也為100.0%;模型St45能正確區(qū)分出St45的敏感性和特異性均為100.0%,模型St239能正確區(qū)分出St239的敏感性是95.8%以及特異性為93.2%,模型St59能正確區(qū)分出St59的敏感性為86.7%以及特異性為91.7%,模型St5能正確區(qū)分出St5的敏感性為50.0%以及特異性為95.8%。結(jié)論 MALDI-TOF MS分型與MLST具有較好的一致性,且具有操作簡便、快速、低成本、重復性好、穩(wěn)定性高的優(yōu)點。
[Abstract]:Objective to evaluate the effect of matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) on methicillin-resistant staphylococcus aureus MRSAs. Methods collected from Shanghai from January 2009 to March 2012. There were 270 MRSAs in Chongqing, Guangzhou, Shenyang, Urumqi and Beijing. The four genotypes of St45 were established by ClinProTools software, and the sensitivity and specificity of the model were obtained by using residual strains. 35 strains of St239 were randomly selected as experimental group, 43 strains of control group including 20 strains of St5N15 strains of St59 strain and 8 strains of St45 were selected to construct St239 model. Eight St45 strains were randomly selected in the test group, and 35 strains in the control group, including 11 strains St5, 8 strain St59, 16 strains of St239. Results St45154 strains of St239 were used as the model. The cross validity and acceptability of the model St239S45F5 were 100.0%, respectively, according to the St45 analysis of 72 St5O30 strains and 14 strains of St59.The results showed that both the cross validity and the acceptability of the model were 100.0%. Only the cross validity of model St59 was 90.5 and its acceptability was 100.0; The sensitivity and specificity of model St45 to distinguish St45 were 100.0%. The sensitivity and specificity of model St239 were 95.8% and 93.2%, respectively. The sensitivity and specificity of model St59 were 86.7% and 91.7% respectively. The sensitivity of St5 was 50.0% and the specificity was 95.8. conclusion the classification of MALDI-TOF MS is consistent with that of MLST. It has the advantages of simple operation, fast operation, low cost, good repeatability and high stability.
【作者單位】： 遼寧醫(yī)學院沈陽軍區(qū)總醫(yī)院研究生基地;遼寧中醫(yī)藥大學附屬醫(yī)院檢驗科;沈陽軍區(qū)總醫(yī)院檢驗科;沈陽軍區(qū)總醫(yī)院科訓科;
【基金】：遼寧省科技攻關計劃基金資助項目(2011225021)
【分類號】：R446.5
【正文快照】： 耐甲氧西林金黃色葡萄球菌(MRSA)是臨床抗感染治療的難點之一,MRSA具有傳播途徑廣、致病性強的特點,MRSA的克隆性流行一直是醫(yī)院感染控制工作的監(jiān)測重點,通常使用的監(jiān)測手段為對分離菌株進行分子生物學分型。常用方法有多位點序列分析(MLST)、脈沖場凝膠電泳分析(PFGE)等,這些，

本文編號：1419719