本文關(guān)鍵詞：轉(zhuǎn)化生長因子-β1和血管內(nèi)皮生長因子在濃縮生長因子各層中的分布及含量特點(diǎn)　出處：《北京大學(xué)學(xué)報(醫(yī)學(xué)版)》2016年05期 　論文類型：期刊論文

  更多相關(guān)文章： 濃縮生長因子 轉(zhuǎn)化生長因子β 血管內(nèi)皮生長因子 骨生成

【摘要】：目的:測定濃縮生長因子(concentrated growth factors,CGF)凝膠層與紅細(xì)胞(red blood cells,RBC)層中轉(zhuǎn)化生長因子-β1(transforming growth factor-β1,TGF-β1)、血管內(nèi)皮生長因子(vascular endothelial growth factor,VEGF)分布及含量,探究TGF-β1、VEGF在CGF凝膠層內(nèi)的分布及含量與其促成骨效應(yīng)的相關(guān)性并指導(dǎo)臨床應(yīng)用。方法:招募6名健康志愿者,采集靜脈血制備CGF樣品。免疫組織化學(xué)法觀察CGF凝膠層與RBC層中TGF-β1、VEGF的分布并測定制備即刻的累積光密度值(integrated optical density,IOD)和平均光密度值(average optical density,AOD)。酶聯(lián)免疫吸附(enzyme-linked immunosorbent assays,ELISA)測定制備即刻上清層與制備1 d后CGF凝膠層析出液中TGF-β1、VEGF的濃度。結(jié)果:TGF-β1與VEGF大量聚集于CGF凝膠層中,在RBC層中僅位于多核細(xì)胞胞漿和散在的血小板內(nèi);CGF凝膠層與RBC層的交界區(qū)域有大量血小板與白細(xì)胞聚集,并有較高的TGF-β1表達(dá);CGF凝膠層析出液中TGF-β1和VEGF的濃度分別為(55 236.78±3 686.34)ng/L和(610.99±148.81)ng/L,均顯著高于上清層(20 710.20±4 523.14)ng/L和(335.20±51.69)ng/L(P0.001)。結(jié)論:CGF凝膠層內(nèi)含有大量TGF-β1、VEGF,可發(fā)揮促進(jìn)成骨與加速組織愈合的作用。在臨床應(yīng)用中建議應(yīng)用時間宜早為佳,除整個CGF層外,可將上清層、CGF層與RBC層交界區(qū)域一并使用。
[Abstract]:Objective: to determine the gel layer of concentrated growth factor concented growth factors (CGF) and red blood cells in erythrocytes. Transforming growth factor- 尾 1 (TGF- 尾 1). The distribution and content of vascular endothelial growth factor (VEGF) in vascular endothelial growth factor.TGF- 尾 1 was studied. The relationship between the distribution and content of VEGF in CGF gel layer and its effect on bone and guiding clinical application. Methods: 6 healthy volunteers were recruited. CGF samples were collected from venous blood, and TGF- 尾 1 in CGF gel layer and RBC layer were observed by immunohistochemical method. The distribution of VEGF and the immediate cumulative optical density of the prepared optical density were measured. IOD) and average optical density. Enzyme linked immunosorbent assays. TGF- 尾 1 in CGF gel chromatography solution was determined by Elisa. The concentration of VEGF. Results: TGF- 尾 1 and VEGF were clustered in the CGF gel layer, and in the RBC layer only in the cytoplasm of the multicellular cells and in the scattered platelets. A large number of platelets and leukocytes were accumulated in the junction region of CGF gel layer and RBC layer, and the expression of TGF- 尾 1 was higher. The concentrations of TGF- 尾 1 and VEGF in CGF gel chromatography solution were 55 236.78 鹵3 686.34 ng / L and 610.99 鹵148.81, respectively. Ng/L. Both were significantly higher than those in the upper clear layer (20 710.20 鹵4 523.14 ng / L and 335.20 鹵51.69 ng / L P 0.001). Conclusion: TGF- 尾 1 was found in the gel layer of CGF. VEGF, which can promote osteogenesis and accelerate tissue healing, should be applied early in clinical application, except for the whole CGF layer, the supernatant layer can be added. The CGF layer is used in conjunction with the RBC layer.
【作者單位】： 北京大學(xué)口腔醫(yī)學(xué)院·口腔醫(yī)院修復(fù)科口腔數(shù)字化醫(yī)療技術(shù)和材料國家工程實(shí)驗(yàn)室口腔數(shù)字醫(yī)學(xué)北京市重點(diǎn)實(shí)驗(yàn)室;中國人民解放軍總醫(yī)院口腔科;
【基金】：國家臨床重點(diǎn)�？平ㄔO(shè)項(xiàng)目(2011) 教育部留學(xué)回國人員科研啟動基金(2012-940)資助~~
【分類號】：R446.6
【正文快照】： 濃縮生長因子(concentrated growth factors,CGF)是繼富血小板血漿(platelet-rich plasma,PRP)、富血小板纖維蛋白(platelet-rich fibrin,PRF)后的新一代血小板濃縮產(chǎn)物,由Saccol于2006年首先提出[1],其包含了發(fā)揮骨誘導(dǎo)作用的自體血小板生長因子和發(fā)揮骨引導(dǎo)作用的纖維蛋白基

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