發(fā)布時間：2018-01-11 18:08

  本文關(guān)鍵詞：線粒體靶向抗氧化劑SS-31肽對膿毒血癥小鼠急性肺損傷的影響　出處：《臨床麻醉學(xué)雜志》2016年05期 　論文類型：期刊論文

  更多相關(guān)文章： 膿毒癥 肺損傷 氧化應(yīng)激 SS-肽

【摘要】：目的觀察線粒體靶向抗氧化劑SS-31肽對小鼠膿毒血癥引起急性肺損傷(ALI)的影響。方法采用盲腸結(jié)扎穿孔法(CLP)建立小鼠膿毒癥模型。成年雄性C57BL/6小鼠48只,體重25~32g,隨機均分為四組:假手術(shù)+溶劑組(A組)、假手術(shù)+SS-31肽組(B組)、CLP+溶劑組(C組)及CLP+SS-31肽組(D組)。于術(shù)后即刻及5hB、D組腹腔注射SS-31肽5mg/kg,A、C組腹腔注射等容劑量的生理鹽水。假手術(shù)或CLP 24h后,采用HE染色法進行肺組織學(xué)評分,取肺組織檢測腫瘤壞死因子-α(TNF-α)、白細胞介素-6(IL-6)、IL-10、丙二醛(MDA)、超氧化物歧化酶(SOD)、髓過氧化物酶(MPO)活性、干濕重比(W/D)、活性氧簇(ROS)、ATP水平、核轉(zhuǎn)錄因子κB(NF-κB)p65、誘導(dǎo)性一氧化氮合酶(iNOS)表達。結(jié)果 C組肺炎癥滲出、水腫程度均明顯重于A組(P0.05),D組肺組織充血、炎性細胞浸潤、肺泡壁水腫明顯輕于C組(P0.05);C組肺組織學(xué)評分、IL-6濃度、MDA、MPO、W/D、ROS水平、NF-κB p65及iNOS表達明顯高于A組,ATP水平明顯低于A組(P0.05);D組肺組織學(xué)評分、IL-6濃度、MDA、MPO、W/D、ROS水平、NF-κB p65及iNOS表達明顯低于C組,ATP水平明顯高于C組(P0.05)。A組與B組各項指標(biāo)差異無統(tǒng)計學(xué)意義。四組肺組織TNF-α、IL-10濃度及SOD水平差異無統(tǒng)計學(xué)意義。結(jié)論 SS-31肽可改善小鼠膿毒血癥引起的ALI,這可能是通過抑制膿毒血癥的炎性反應(yīng)和氧化應(yīng)激實現(xiàn)的。
[Abstract]:Objective to observe the effect of mitochondrial targeted antioxidant SS-31 peptide on acute lung injury induced by sepsis in mice. The sepsis model was established in 48 adult male C57BL / 6 mice. The body weight was 25 ~ 32g. They were randomly divided into four groups: sham-operation solvent group (group A) and sham-operation group (group B with SS-31 peptide). CLP solvent group (C group) and CLP SS-31 peptide group (group D) were injected intraperitoneally with SS-31 peptide 5 mg / kg at 5 h after operation. In group C, saline of equal volume was injected intraperitoneally. 24 hours after sham-operation or CLP, lung histology was evaluated by HE staining method, and tumor necrosis factor- 偽 (TNF- 偽) was detected in lung tissue. The activity of IL-10, malondialdehyde (MDAA), superoxide dismutase (SOD), myeloperoxidase (MPO) and dry wet weight ratio (WR) were observed. The level of ATP, the expression of nuclear transcription factor 魏 B, NF- 魏 B, inducible nitric oxide synthase (iNOS) and the expression of ATP in Ros were observed. Results the pulmonary inflammation exudated in group C. The degree of edema was significantly more severe than that of group A (P 0.05) and the infiltration of inflammatory cells, and the edema of alveolar wall was significantly less than that of group C (P 0.05). The expression of NF- 魏 B p65 and iNOS in group C was significantly higher than that in group A (P < 0.05). The level of ATP in group A was significantly lower than that in group A (P 0.05). Lung histological score and IL-6 concentration in group D were significantly lower than those in group C (P < 0.05). The expression of NF- 魏 B p65 and iNOS was significantly lower than that in group C. The level of ATP in group C was significantly higher than that in group C. there was no significant difference between group A and group B. TNF- 偽 in lung tissue of four groups. There was no significant difference in IL-10 concentration and SOD level. Conclusion SS-31 peptide can improve ALI induced by sepsis in mice. This may be achieved by inhibiting inflammatory responses to sepsis and oxidative stress.
【作者單位】： 江蘇大學(xué)附屬金壇醫(yī)院ICU;江蘇省麻醉學(xué)重點實驗室江蘇省麻醉與鎮(zhèn)痛應(yīng)用技術(shù)重點實驗室;南京軍區(qū)南京總醫(yī)院麻醉科;
【基金】：江蘇大學(xué)醫(yī)學(xué)臨床科技發(fā)展基金項目(JLY2012008-1) 江蘇金壇市科技計劃項目(JT2013065)
【分類號】：R459.7
【正文快照】： 作者單位:213200江蘇大學(xué)附屬金壇醫(yī)院ICU(李國民、丁東亮、袁冬);江蘇省麻醉學(xué)重點實驗室江蘇省麻醉與鎮(zhèn)痛應(yīng)用技術(shù)重點實驗室(楊嬌嬌);南京軍區(qū)南京總醫(yī)院麻醉科(吳晶、楊建軍)急性肺損傷(acute lung injury,ALI)是指各種肺內(nèi)外致病因素所導(dǎo)致的急性、進行性的呼吸功能不全,

【相似文獻】

相關(guān)期刊論文 前10條

1 魏文峰;;中樞神經(jīng)系統(tǒng)感染與膿毒血癥[J];交通醫(yī)學(xué);2014年01期

2 林偉平;李素珍;張秋芳;;高齡膿毒血癥患者白細胞急劇下降一例的護理[J];解放軍護理雜志;2008年17期

3 馮亭;;膿毒血癥13例臨床護理[J];齊魯護理雜志;2010年28期

4 戴喜明;;類鼻疽伯克霍爾德菌重癥肺炎并發(fā)膿毒血癥1例[J];臨床急診雜志;2012年03期

5 黃彩英;;1例幼兒燙傷后嚴重感染膿毒血癥的搶救和護理體會[J];大家健康(學(xué)術(shù)版);2012年21期

6 ;診治一例膿毒血癥的經(jīng)驗教訓(xùn)[J];新醫(yī)學(xué);1973年09期

7 陳建華;海綿竇膿毒血癥的觀察護理[J];護士進修雜志;1993年02期

8 吳建國;劉彥伶;賈蓓;黃文祥;;耐甲氧西林金黃色葡萄球菌膿毒血癥復(fù)發(fā)1例[J];中國感染與化療雜志;2014年02期

9 戴吉;陸俊杰;周敏;;血漿腦鈉肽對嚴重膿毒血癥預(yù)后判斷的臨床意義[J];當(dāng)代醫(yī)學(xué);2014年10期

10 申吉泓;張白羽;;泌尿外科醫(yī)生必須高度重視的尿源性膿毒血癥[J];現(xiàn)代泌尿外科雜志;2014年04期

相關(guān)會議論文 前6條

1 胡靜;陸國平;陸鑄今;盧亞芳;賀駿;張靈恩;;新生兒膿毒血癥血液凈化的護理[A];中華醫(yī)學(xué)會第十四次全國兒科學(xué)術(shù)會議論文匯編[C];2006年

2 胡靜;陸國平;陸鑄今;盧亞芳;賀駿;張靈恩;;新生兒膿毒血癥血液凈化的護理[A];2006（第三屆）江浙滬兒科學(xué)術(shù)會議暨浙江省兒科學(xué)術(shù)年會論文匯編[C];2006年

3 劉長文;鄭永科;陸駿;朱克毅;胡煒;胡偉航;;高容量血液濾過對膿毒血癥合并急性呼吸窘迫綜合征的呼吸、血液動力學(xué)和氧代謝的影響[A];2006年浙江省危重病學(xué)學(xué)術(shù)年會論文匯編[C];2006年

4 吳亮;龔仕金;嚴靜;戴海文;陳進;蔡國龍;虞意華;張召才;;血漿降鈣素原檢測對老年膿毒血癥的早期診斷價值[A];2005年浙江省危重病學(xué)學(xué)術(shù)年會論文匯編[C];2005年

5 田恬;;3例因結(jié)石梗阻造成尿膿毒血癥行輸尿管鏡下雙J管置入治療的護理[A];2013年貴州省泌尿外科學(xué)術(shù)會議論文匯編[C];2013年

6 張志飛;;洱源性膿毒血癥并發(fā)膿胸一例[A];科技創(chuàng)輝煌——中國創(chuàng)新成果與學(xué)術(shù)精典[C];2011年

相關(guān)博士學(xué)位論文 前2條

1 唐e，

本文編號：1410625