本文關(guān)鍵詞：液相色譜-動(dòng)態(tài)串聯(lián)質(zhì)譜技術(shù)同時(shí)檢測人尿中120種刺激劑和利尿劑等禁用物質(zhì)　出處：《中國運(yùn)動(dòng)醫(yī)學(xué)雜志》2016年05期 　論文類型：期刊論文

  更多相關(guān)文章： 液相色譜-動(dòng)態(tài)串聯(lián)質(zhì)譜 直接進(jìn)樣 刺激劑 利尿劑 興奮劑管制

【摘要】：目的:采用液相色譜-動(dòng)態(tài)串聯(lián)質(zhì)譜技術(shù),建立和驗(yàn)證同時(shí)檢測人尿樣中120種刺激劑、利尿劑和其它禁用物質(zhì)的方法。方法:尿樣經(jīng)同等體積的5%醋酸鉛水溶液沉淀蛋白質(zhì)后,上清液用動(dòng)態(tài)多反應(yīng)監(jiān)測模式(d MRM)進(jìn)行檢測,并比較了傳統(tǒng)MRM與動(dòng)態(tài)MRM在檢測靈敏度上的差異。結(jié)果:所篩查禁用物質(zhì)的檢測限均介于0.05~20 ng/ml之間,達(dá)到世界反興奮劑機(jī)構(gòu)對禁用物質(zhì)檢測能力的要求。在低、中和高三個(gè)濃度級別的日內(nèi)和日間精密度均小于20%,準(zhǔn)確度和基質(zhì)效應(yīng)在80%~120%之間。結(jié)論:該方法具有靈敏度高、準(zhǔn)確可靠和重現(xiàn)性好等特點(diǎn)。動(dòng)態(tài)MRM模式只掃描某個(gè)禁用物質(zhì)保留時(shí)間附近區(qū)域,駐留時(shí)間大大增加,其檢測靈敏度遠(yuǎn)高于傳統(tǒng)MRM模式,更適合于多種禁用物質(zhì)的同時(shí)檢測。
[Abstract]:Objective: using liquid chromatography tandem mass spectrometry dynamic technology, establish and validate the simultaneous detection of 120 kinds of stimuli in human urine, method of diuretics and other prohibited substances. Methods: urine samples were 5% lead acetate solution of the same volume of precipitated protein, by using the dynamic multiple reaction monitoring mode (D and MRM) were detected. Compare the difference between traditional MRM and MRM in the dynamic detection sensitivity. Results: between screening detection limit substances are disabled from 0.05~20 to ng/ml, to meet the requirements of the World Anti Doping Agency banned substances detection ability. In the low and high concentration levels of intra day and inter day precision were less than 20% accuracy and the matrix effect between 80%~120%. Conclusion: the method is sensitive, accurate and reproducible. Dynamic MRM scan mode only a prohibited substance retention time near the area, the dwell time greatly In addition, its detection sensitivity is much higher than that of the traditional MRM model, which is more suitable for simultaneous detection of a variety of prohibited substances.

【作者單位】： 國家體育總局反興奮劑中心;
【基金】：國家體育總局反興奮劑中心科研項(xiàng)目(2013ZR002)
【分類號】：R872;R446.12
【正文快照】： 刺激劑和利尿劑類藥物是競技體育中經(jīng)常被濫用的興奮劑。刺激劑通過對中樞神經(jīng)系統(tǒng)的選擇性作用,能增強(qiáng)機(jī)體活力和敏捷性,還可使運(yùn)動(dòng)員疲勞感減輕,自信心增強(qiáng),注意力集中。利尿劑可促進(jìn)腎臟排尿功能,具有快速減輕體重的作用,主要用于需要控制體重的運(yùn)動(dòng)項(xiàng)目如拳擊、摔跤、舉重，

本文編號：1395904