生物醫(yī)學(xué)工程的理想生物材料應(yīng)模仿天然組織的內(nèi)在特性,特別是要有一定的機(jī)械強(qiáng)度,使得細(xì)胞能夠有效附著。此外,優(yōu)異的細(xì)胞親和力和組織粘附性使得植入后能夠與周圍組織整合。有機(jī)/無機(jī)生物啟發(fā)的納米復(fù)合材料具有突出的機(jī)械和生物化學(xué)性質(zhì),已經(jīng)進(jìn)行了廣泛的研究,盡管將生物相容性等功能性與機(jī)械性能相結(jié)合是一項(xiàng)挑戰(zhàn),具有多功能性。受大自然的啟發(fā),我們通過逐層方法開發(fā)了使用聚丙烯酸（PAH）,聚（酰氨基胺）（PAMAM）和納米碳酸鈣（納米碳酸鈣）的聚電解質(zhì)的納米復(fù)合材料,在生物醫(yī)學(xué)領(lǐng)域具有潛在的應(yīng)用。使用原子力顯微鏡（AFM）,掃描電子顯微鏡（SEM）,透射電子顯微鏡（TEM）,X射線衍射（XRD）,傅里葉變換紅外光譜（FTIR）和拉曼光譜表征制備的混合納米復(fù)合膜。此外,評(píng)估了復(fù)合膜的機(jī)械和功能特性。在礦化之后,合成的復(fù)合膜顯示出顯著增強(qiáng)的機(jī)械性能,與平均楊氏模量為5.2GPa的非礦化復(fù)合膜相比,平均楊氏模量為10.6GPa。此外,與對(duì)照樣品相比,礦化復(fù)合膜顯示出改善的壓電性質(zhì)。使用LIVE/DEAD測(cè)定法在人肺成纖維細(xì)胞（hLFC）上測(cè)試復(fù)合材料的細(xì)胞相容性評(píng)估,結(jié)果表明對(duì)細(xì)胞的毒性可忽略不計(jì)。細(xì)胞附著... 

【文章來源】：中國(guó)地質(zhì)大學(xué)(北京)北京市 211工程院校 教育部直屬院校

【文章頁數(shù)】：69 頁

【學(xué)位級(jí)別】：碩士

【文章目錄】：
List of Abbreviations
摘要
Abstract
Chapter 1 Introduction
    1.1 Research background and significance of the study
        1.1.1 LbL Self Assembly Technology
        1.1.2 Dendrimers
        1.1.3 Calcium carbonate
    1.2 Research history and present situation
        1.2.1 PAA/ CaCO_3 films
        1.2.2 Protein/ CaCO_3 films
        1.2.3 Cellulose derivatives/ CaCO_3 films
        1.2.4 Other organic/ CaCO_3 films
    1.3 Purpose and significant of research
        1.3.1 Research purpose
        1.3.2 Significant of research
    1.4 Research content
Chapter 2 Fabrication of LbL polyelectrolyte (PAH/PAMAM)_(7.5) multilayer film
    2.1 Introduction
    2.2 Preparation of LbL polyelectrolyte (PAH/PAMAM)_(7.5) multilayer film
        2.2.1 Experimental raw materials
        2.2.2 Experimental instruments and equipment
        2.2.3 Experimental procedure and sample preparation
            2.2.3.1 Preparation of the Piezoelectric Composite Film PVDF-HFP
            2.2.3.2 Fabrication of the Crosslinked PAH/PAMAM Multilayers on the Piezoelectric Film/Quartz/ITOSubstrates
    2.3 Results and Discussions
        2.3.1 Film Preparation
    2.4 Summary of this chapter
Chapter 3 Fabrication and characterization of CaCO_3 on (PAH/PAMAM)_(7.5)- multilayer film
    3.1 Introduction
    3.2 Preparations and characterization of CaCO_3 on (PAH/PAMAM)_(7.5) multilayer film
        3.2.1 Experimental raw materials
        3.2.2 Experimental instruments and equipment
        3.2.3 Experimental procedure and sample preparation
            3.2.3.1 Mineralization of CaCO_3 nano particles in the LbL PAH/PAMAM matrices
            3.2.3.2 Film thickness, Morphological and compositional characterizations
    3.3 Results and Discussions
        3.3.1 Film thickness, Morphological characterizations
        3.3.2 Composition analysis
    3.4 Summary of this chapter
Chapter 4 Property characterizations of (PAH/PAMAM)_(7.5)-CaCO_3 multilayer film
    4.1 Introduction
    4.2 Property characterization of CaCO_3 on (PAH/PAMAM)_(7.5) multilayer film
        4.2.1 Experimental raw materials
        4.2.2 Experimental instruments and equipment
        4.2.3 Experimental procedure and sample preparation
            4.2.3.1 Mechanical properties of (PAH/PAMAM)_(7.5) before and after mineralization
            4.2.3.2 Electrical properties of (PAH/PAMAM)_(7.5) before and after mineralization
    4.3 Results and Discussions
        4.3.1 Young's moduli
        4.3.2 Piezoelectric Properties
        4.3.3 Electrochemical impedance spectroscopy (EIS)
    4.4 Summary of this chapter
Chapter 5 Applications of (PAH/PAMAM)_(7.5)-CaCO_3 multilayer film
    5.1 Introduction
    5.2 Test Instruments
        5.2.1 Experimental raw materials
        5.2.2 Experimental instruments and equipment
        5.2.3 Experimental procedure and sample preparation
            5.2.3.1 The Loading and Release of Bio macro molecules
            5.2.3.2 Cell culture and Cytocompatibility test
    5.3 Results and Discussions
        5.3.1 Molecular load and release of Methylene blue (MB) into the matrix before and after mineralization
        5.3.2 Molecular load and release of Gentamycin Sulfate (GS) into the matrix after mineralization
        5.3.3 Cell culture and Cytocompatibility
    5.4 Summary of this chapter
Chapter 6 Conclusions
    6.1 Overall conclusions
    6.2 The main innovation of this paper
    6.3 Future works
References
Acknowledgements



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