本文選題：微球透鏡 + 超分辨成像�。� 參考：《南京師范大學(xué)》2017年碩士論文

【摘要】：眾所周知,光學(xué)顯微技術(shù)在科學(xué)研究中占據(jù)舉足輕重地位。但是受到傳統(tǒng)光學(xué)衍射極限影響,傳統(tǒng)光學(xué)顯微鏡的分辨率大約只有照明波長一半。近年來,借助介質(zhì)微球突破衍射極限成為實(shí)現(xiàn)超分辨成像一種簡便有效的方法。本文提出了一種簡單有效的超分辨薄膜制備方法,并對(duì)基于介質(zhì)微球的超分辨薄膜成像特性進(jìn)行了研究。本論文的主要內(nèi)容包括:(1)本文提出了一種新的基于介質(zhì)微球超分辨薄膜制備方法,通過使用普通透明膠背面作為薄膜襯底,制備了厚度為幾個(gè)微米的超分辨薄膜。(2)研究了浸沒在不同厚度聚二甲基硅氧烷(Polydimethylsiloxane,PDMS)薄膜中的鈦酸鋇(Barium Titanate Glass,BTG)微球成像特性。實(shí)驗(yàn)結(jié)果顯示,隨著PDMS薄膜厚度增加,成像放大倍數(shù)變化不大,但視場(chǎng)變化顯著。當(dāng)PDMS薄膜厚度在5-10 μm時(shí),微球視場(chǎng)達(dá)到最大。為分析視場(chǎng)大小變化的原因,我用Computer Simulation Technology (CST)軟件對(duì)不同厚度PDMS薄膜進(jìn)行模擬仿真。此外,我還制備了 7.4μm厚度的PDMS薄膜,分別研究了 2, 3, 4和6個(gè)微球緊密排列時(shí)的超分辨成像特性。(3)在微球透鏡與樣品表面增加一層介質(zhì)層,研究了不同厚度的SU-8光刻膠對(duì)微球成像特性的影響。實(shí)驗(yàn)發(fā)現(xiàn):隨著SU-8厚度從0增大到270 nm,成像視場(chǎng)大小從10.8 μm增加到了 13.2μm。當(dāng)SU-8厚度在160nm左右時(shí)候,成像視場(chǎng)大小與對(duì)比度最優(yōu)。此外,對(duì)于厚度為160 nm的不同材料介質(zhì)層,發(fā)現(xiàn)SU-8最能提高視場(chǎng)大小,而二氧化鈦(TiO2)介質(zhì)層卻削弱了視場(chǎng)大小。我認(rèn)為耦合進(jìn)介質(zhì)層的光信息含量影響了成像質(zhì)量,并通過光波干涉相消對(duì)這一現(xiàn)象進(jìn)行了分析。
[Abstract]:As we all know, optical microscopy plays an important role in scientific research. However, the resolution of traditional optical microscopy is about half of the lighting wavelength. In recent years, it is a simple and effective method to achieve superresolution imaging with the help of medium microspheres to break through the diffraction limit. A simple and effective super-resolution film preparation method and the characteristics of super-resolution film imaging based on dielectric microspheres are studied. The main contents of this paper include: (1) a new preparation method based on dielectric microsphere super-resolution film is proposed. The thickness is prepared by using the back of the ordinary transparent adhesive as the film substrate. (2) the imaging characteristics of barium titanate (Barium Titanate Glass, BTG) microspheres immersed in Polydimethylsiloxane (PDMS) thin films with different thickness are studied. The experimental results show that with the increase of the thickness of the PDMS film, the large multiplier of the imaging has not changed much, but the field of view changes remarkably. When the thickness of the PDMS film is in the thickness of the PDMS film, At 5-10 m, the field of view reaches the maximum. In order to analyze the cause of the change of field of view, I use Computer Simulation Technology (CST) software to simulate the different thickness of PDMS film. In addition, I also prepared the PDMS thin film with the thickness of 7.4 mu m, and studied the super-resolution imaging characteristics of 2, 3, 4 and 6 microspheres respectively. (3) The microsphere lens and the sample surface add a layer of media to study the effect of SU-8 photoresist on the imaging characteristics of the microspheres with different thickness. The experimental results show that as the thickness of SU-8 increases from 0 to 270 nm, the image field size is increased from 10.8 to 13.2 to M., when the thickness of SU-8 is around 160nm, the size and contrast of the imaging field is optimal. In different material layers with a thickness of 160 nm, it is found that SU-8 can improve the field of view most, while the titanium dioxide (TiO2) layer weakens the size of the field of view. I think the optical information content of the coupled dielectric layer affects the imaging quality, and the phenomenon is analyzed by the interference of light wave interference.
【學(xué)位授予單位】：南京師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：O484

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相關(guān)期刊論文 前2條

1 龐輝;杜春雷;邱琪;鄧啟凌;張滿;尹韶云;;介質(zhì)微球超分辨成像薄膜[J];光子學(xué)報(bào);2015年04期

2 王淑瑩;章海軍;張冬仙;;基于微球透鏡的任選區(qū)高分辨光學(xué)顯微成像新方法研究[J];物理學(xué)報(bào);2013年03期

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本文編號(hào)：1974266