牛鞭草屬DUS測(cè)試材料的SSR指紋圖譜構(gòu)建
發(fā)布時(shí)間：2019-03-18 20:05
【摘要】：高效快速的品種(系)DNA指紋圖譜鑒定,可為輔助牛鞭草屬品種(系)DUS測(cè)試和知識(shí)產(chǎn)權(quán)保護(hù)等提供科學(xué)依據(jù)。本研究從54對(duì)SSR引物中篩選出20對(duì)引物對(duì)牛鞭草屬25個(gè)DUS測(cè)試材料進(jìn)行鑒定分析。結(jié)果表明,20對(duì)SSR引物對(duì)牛鞭草屬25個(gè)DUS測(cè)試材料共擴(kuò)增出253條清晰條帶,其中多態(tài)性條帶241條,多態(tài)性條帶比率(PPB)為95.26%。每對(duì)引物的多態(tài)信息含量(PIC)為0.599~0.900,平均為0.760。UPGMA聚類分析表明,牛鞭草屬25個(gè)材料在相似系數(shù)為0.668處可分為三大類群。20對(duì)SSR引物能鑒別10~22個(gè)牛鞭草屬材料,引物DHSSR7、引物DHSSR11和引物DHSSR15的有效組合可鑒別所有供試材料。以3對(duì)SSR引物擴(kuò)增的電泳圖譜為基礎(chǔ),建立了牛鞭草屬25個(gè)DUS測(cè)試材料的指紋圖譜標(biāo)準(zhǔn)模式圖,能夠快速區(qū)分各個(gè)供試材料。
[Abstract]:The high efficiency and rapid identification of DNA fingerprinting can provide scientific basis for assisting DUS testing and intellectual property protection of bullwhip varieties (lines). In this study, 20 pairs of SSR primers were screened out from 54 pairs of primers to identify and analyze 25 DUS test materials of the genus Verbena. The results showed that a total of 253clear bands were amplified by 20 pairs of SSR primers against 25 DUS materials, of which 241were polymorphic bands, and the ratio of polymorphic bands was 95.26%. The polymorphic information content (PIC) of each pair of primers was 0.599 ~ 0.900, and the average 0.760.UPGMA cluster analysis showed that: Twenty-two pairs of SSR primers could be used to identify 10 and 22 materials, and the effective combination of primer DHSSR7, primer DHSSR11 and primer DHSSR15 could be used to identify all of the tested materials. The similarity coefficient of the 25 materials could be divided into three groups at the similarity coefficient of 0.668. 20 pairs of primers could be used to identify 10 to 22 materials of the genus Botrytis. Based on the electrophoresis patterns amplified by three pairs of SSR primers, the standard patterns of fingerprint patterns of 25 DUS materials of the genus bullwhip were established, which could quickly distinguish the tested materials from each tested material.
【作者單位】： 四川農(nóng)業(yè)大學(xué)草業(yè)科學(xué)系;
【基金】：國(guó)家牧草產(chǎn)業(yè)體系(CARS-35-05) 四川省育種攻關(guān)項(xiàng)目(2016NYZ0039)共同資助
【分類號(hào)】：Q943.2;S543.9
本文編號(hào)：2443190
[Abstract]:The high efficiency and rapid identification of DNA fingerprinting can provide scientific basis for assisting DUS testing and intellectual property protection of bullwhip varieties (lines). In this study, 20 pairs of SSR primers were screened out from 54 pairs of primers to identify and analyze 25 DUS test materials of the genus Verbena. The results showed that a total of 253clear bands were amplified by 20 pairs of SSR primers against 25 DUS materials, of which 241were polymorphic bands, and the ratio of polymorphic bands was 95.26%. The polymorphic information content (PIC) of each pair of primers was 0.599 ~ 0.900, and the average 0.760.UPGMA cluster analysis showed that: Twenty-two pairs of SSR primers could be used to identify 10 and 22 materials, and the effective combination of primer DHSSR7, primer DHSSR11 and primer DHSSR15 could be used to identify all of the tested materials. The similarity coefficient of the 25 materials could be divided into three groups at the similarity coefficient of 0.668. 20 pairs of primers could be used to identify 10 to 22 materials of the genus Botrytis. Based on the electrophoresis patterns amplified by three pairs of SSR primers, the standard patterns of fingerprint patterns of 25 DUS materials of the genus bullwhip were established, which could quickly distinguish the tested materials from each tested material.
【作者單位】： 四川農(nóng)業(yè)大學(xué)草業(yè)科學(xué)系;
【基金】：國(guó)家牧草產(chǎn)業(yè)體系(CARS-35-05) 四川省育種攻關(guān)項(xiàng)目(2016NYZ0039)共同資助
【分類號(hào)】：Q943.2;S543.9
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