本文關(guān)鍵詞：蒺藜莖葉化學(xué)成分及含量測定研究，，由筆耕文化傳播整理發(fā)布。

        蒺藜(Tribulus terrestris L.),為蒺藜科(Zygophylaceae)蒺藜屬(Tribulus)植物。蒺藜莖葉又名蒺藜苗,始載于《本草綱目》,具有祛風(fēng)、消腫作用,主治癰腫,疥癬,風(fēng)癢,鼻塞等。蒺藜在我國具有悠久的藥用歷史,素有“草中名藥”之稱,其果實(shí)、花、根等也均可作為藥用部位。蒺藜主要含有皂苷、黃酮、生物堿和多糖類等化合物,現(xiàn)代藥理研究表明甾體皂苷和黃酮類成分為其主要活性成分,具有改善心腦血管、降血糖、增強(qiáng)性功能、抗衰老、調(diào)血脂、抑菌、抗腫癌等藥理活性。本文首先通過查閱國內(nèi)外大量文獻(xiàn),對蒺藜化學(xué)成分及藥理作用研究現(xiàn)狀進(jìn)行了綜述,共引用153篇文獻(xiàn)。在此基礎(chǔ)上,采用化學(xué)手段以及多種分離手段,包括萃取、大孔樹脂、日本MCI樹脂、羥丙基葡聚糖凝膠色(Sephadex LH20)及PHPLC等分離手段對蒺藜莖葉95%乙醇提取物部位進(jìn)行了化學(xué)成分研究,共分離得到14個化合物,通過理化性質(zhì)及UV、1hnmr、13cnmr、hmbc、hsqc等波譜技術(shù)鑒定出6個化合物,分別為異鼠李素-3-O-β-D-龍膽雙糖苷(isorhamnetin-3-O-β-D-gentiobioside, JL-1),槲皮素-3-O-β-D-龍膽雙糖苷(quercetin-3-O-β-D-gentiobioside, JL-2),山奈酚-3-O-β-D-龍膽雙糖苷(kaemp ferol-3-O-β-D-gentiobioside, JL-3),異鼠李素-3-O-β-D-龍膽雙糖基-7-O-β-D-吡喃葡萄糖苷(isorhamnetin-3-O-β-D-gentiobioside-7-0-P-D-glucoside, JL-4),山奈酚-3-O-β-D-龍膽雙糖基-7-I-β-D-吡喃葡萄糖苷(kaempferol-3-O-β-D-gentiobioside-7-0-β-D-glucoside, JL-5), N-反式-對羥基苯乙基阿魏酰胺-4’-0-βD-葡萄糖苷(N-trans-ferulo yl tyramine-4’-O-β-D-glucopyranoside, JL-6)�；衔颙L-5和JL-6中糖的連接方式首次明確,JL-6為首次從蒺藜科植物中分離得到。JL-1, JL-2及JL-4在蒺藜中含量較高,經(jīng)檢測純度均達(dá)到99%以上,符合對照品的要求。另外,對蒺藜莖葉有效部位的質(zhì)量控制方法進(jìn)行了研究,采用HPLC-DAD法建立了蒺藜莖葉中含量較高的三種黃酮醇苷類化合物的含量測定方法。結(jié)果,異鼠李素-3-O-β-D-龍膽雙糖基-7-O-β-D-葡萄糖苷、槲皮素-3-O-β-D-龍膽雙糖苷及異鼠李素-3-O-β-D-龍膽雙糖苷分別在0.56～14.00、3.24～129.60及0.92～23.00μg/mL范圍內(nèi)線性良好,其回歸方程分別為Y=21388X-2087.8, r=0.9996; Y=34596X-37335, r=0.9998; Y=50667X+10439, r=0.9998。本文通過對蒺藜莖葉化學(xué)成分的研究,從分離出的黃酮化合物中,選擇含量較高的三個黃酮醇類化合物作為對照品。利用反相HPLC法同時檢測了異鼠李素-3-O-β-D-龍膽雙糖基-7-O-β-D-葡萄糖苷、槲皮素-3-O-β-D-龍膽雙糖苷及異鼠李素-3-O-βp-D-龍膽雙糖苷的含量,該法具有簡便、快速、穩(wěn)定可靠、靈敏的特點(diǎn),精密度及重現(xiàn)性良好,回收率符合要求,可為蒺藜或其制劑的質(zhì)量控制和綜合評價(jià)提供參考。

    The plant Tribulus terrestris L., belonging to the family Zygophylianceae, have been used in traditional Chinese medicine against various diseases. The stems and leaves of Tribulus terrestris L., also known as "Jili-miao", only contained in the "Shen Nong’s Herbal Classic" are used for treating edema, abdominal distension and so on. As we all know, besides the magical therapeutic effect of the fruit of Tribulus terrestris L., its roots, flowers, stems and leaves can also be used as medicine parts. Tribulus terrestris L. has contained numerous compounds such as saponins, flavonoids, alkaioids and polysaccharides. Modern pharmacological studies have shown that steroidal saponins and flavonoids are mainly active constituents, which can improve cardiovascular diseases, lowering blood sugar, enhancing sexual function, anti-aging and lipids, anti-neoplasm pharmaceutical activity.Firstly, the paper summarizes the research situation of chemical constituents and p harmacological activities on the basis of the current domestic and international literatur es (153references were cited). By means of macroporous resin, MCI gel, the Sephade x LH-20, preparative HPLC separation method et al.14compounds were isolated from the95%EtOH extract of stems and leaves. Among them, on the basis of spectroscop y analysis, including UV,1HNMR,13CNMR, HMBC, HSQC and chemical methods,5compound structures were identified as isorhamnetin-3-O-β-D-gentiobioside (JL-1), querc etin-3-O-β-D-gentiobioside (JL-2), kaempferol-3-O-β-D-gentiobioside (JL-3), isorhamnetin-3-O-β-D-gentiobioside-7-O-β-D-glucoside(JL-4), kaempferol-3-O-β-D-gentiobioside-7-O-β-D-glucoside(JL-5), N-trans-feruloyl tyramine-4’-O-β-D-glucopyranoside(JL-6). Among the m, compound JL-6was the first reported in Zygophylaceae. Compounds JL-1, JL-2an d JL-4with the purity of more than99%were chosen as the reference substance. Esta blish a quantitative method for the determination of three flavonols in the effective of the stems and leaves of Tribulus terrestris by HPLC-ADA. The contents of index com pounds including isorhamnetin-3-O-β-D-gentiobioside (JL-1), quercetin-3-O-β-D-gentiobio side (JL-2) and isorhamnetin-3-O-β-D-gentiobioside-7-O-β-D-glucoside(JL-4) were deter mined by HPLC. The calibration curve is linear at ranges of0.56-14.00,3.24～129.60and0.92～23.00μg/mL respectively. Their regression equations are Y=21388X-2087.8, r=0.9996; Y=34596X-37335, r=0.9998; Y=50667X+10439, r=0.9998。In this paper, by the isolation and structural identification of flavonoids compound from the stems and leaves of Tribulus terrestris L, we confirmed higher content of th ree flavonol compounds as reference substance. Establish a method based on reversed phase HPLC was developed to simultaneously determine the content of isorhamnetin-3- O-β-D-gentiobioside, quercetin-3-O-β-D-gentiobioside and isorhamnetin-3-O-β-D-gentiobio side-7-O-P-β-glucoside. This method is considered to be simple, accurate, stable, good precision and reproducibility and is suitable for evaluating the quality of Tribulus terre stris L.

蒺藜莖葉化學(xué)成分及含量測定研究

中文摘要4-6ABSTRACT6-7前言8-10Abbreviations10-12第一部分 文獻(xiàn)綜述12-30    第一節(jié) 概述12    第二節(jié) 蒺藜化學(xué)成分研究進(jìn)展12-20    第三節(jié) 蒺藜藥理作用研究進(jìn)展20-30第二部分 蒺藜莖葉實(shí)驗(yàn)研究部分30-68    第一章 蒺藜莖葉化學(xué)成分研究30-46        第一節(jié) 化學(xué)成分結(jié)構(gòu)解析30-38        第二節(jié) 實(shí)驗(yàn)部分38-46    第二章 蒺藜莖葉黃酮對照品研究46-58        第一節(jié) 對照品結(jié)構(gòu)鑒定46-47        第二節(jié) 對照品純度測定47-57        第三節(jié) 結(jié)果與討論57-58    第三章 HPLC法測定蒺藜莖葉中3個黃酮苷的含量58-68總結(jié)與討論68-70主要創(chuàng)新點(diǎn)70-72參考文獻(xiàn)72-82附圖82-102致謝102-104個人簡歷104

  本文關(guān)鍵詞：蒺藜莖葉化學(xué)成分及含量測定研究，由筆耕文化傳播整理發(fā)布。