本文關(guān)鍵詞：野菊花中萜類和黃酮類化合物保肝作用研究，由筆耕文化傳播整理發(fā)布。

野菊花中萜類和黃酮類化合物保肝作用研究

Protection of terpenes and flavonoids from Chrysanthemi lndici Flos on immunological liver injury

[1] [2] [3] [4] [5] [6] [7] [8]

LI Guo-dong, CHEN Yuan-yuan, WANG Pan, GUAN Ruo-fei, YE Yu-ting, ZHAO Jing, A You-mei, BI Yue-feng（1. School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001,

[1]鄭州大學(xué)藥學(xué)院,河南鄭州450001; [2]鄭州大學(xué)生命科學(xué)學(xué)院,河南鄭州450001

文章摘要：目的 研究野菊花中萜類和黃酮類化合物對刀豆蛋白A（Con A）誘導(dǎo)的小鼠免疫性肝損傷的保護(hù)作用。方法 將小鼠隨機(jī)分成對照組，模型組，野菊花各提取部位（CIF-A，主要含萜類化合物；CIF-B，主要含萜類和黃酮類化合物；CIF-C，主要含黃酮類化合物）高、中、低劑量組，聯(lián)雙酯滴丸陽性對照組。小鼠首日上下午和次日下午各ig給藥1次，以后每天給藥1次，連續(xù)7 d，，末次給藥后，除對照組外，其他組小鼠尾iv Con A制備小鼠免疫性肝損傷模型。檢測小鼠血清中天冬氨酸轉(zhuǎn)氨酶（AST）、丙氨酸轉(zhuǎn)氨酶（ALT）、腫瘤壞死因子-α（TNF-α）、γ干擾素（IFN-γ）的水平；測定肝組織中MDA的量，觀察肝臟病理組織學(xué)變化。結(jié)果 與模型組比較，CIF-A、CIF-B均抑制血清中AST、ALT、TNF-α水平的升高；CIF-C抑制AST、ALT、IFN-γ水平的升高；CIF-B可降低肝組織中MDA的量，明顯改善肝組織病變，作用優(yōu)于CIF-A和CIF-C。結(jié)論 野菊花中萜類和黃酮類化合物對Con A致小鼠免疫性肝損傷均具有一定的保肝作用，CIF-B作用更強(qiáng)。

Abstr：Objective To study the protection of the terpenes and flavonoids from Chrysanthemi Indici Flos （CIF） on immunological liver injury （ILI） induced by concanavalin A （Con A） in mice. Methods Mice were divided into control, model, CIF-A （terpenes）, CIF-B （terpenes and flavonoids）, and CIF-C （flavonoids in high-, mid-, and low-dose） groups, and with Bifendate Pills as positive control group. Mice were ig administered with the three extracts from CIF each once in the morning and afternoon of the first day and the next afternoon, then once daily for consecutive 7 d. The model of ILI was prepared by iv injection of Con A in the tail of mice in each group except the control group. The levels of aspartate aminotransferase （AST）, alanine aminotransferase （ALT）, inferon-α （TNF-α）, and interferon-γ （IFN-γ） in serum of mice were analyzed, the level of MDA in liver was measured, and the histopathological changes of hepatic tissue were observerd. Results Compared with the model group, CIF-A and CIF-B could obviously inhibit the increased levels of AST, ALT, and TNF-α in serum, while CIF-C could mainly reduce the levels of AST, ALT, IFN-γ, and CIF-B could decrease the level of MDA in the liver tissue with the significant amelioration of liver lesions. The effect of CIF-B was stronger than that of CIF-C and CIF-A. Conclusion Terpenes and flavonoids from CIF have a certain protective effect on the liver of mice with ILI induced by Con A, and CIF-B has stronger effect.

文章關(guān)鍵詞：

Keyword：:Chrysanthemi Indici Flos terpenes flavonoids immunological liver injury liver protection

課題項(xiàng)目：國家自然科學(xué)基金面上項(xiàng)目（30973872,81273392）;河南省基礎(chǔ)與前沿技術(shù)研究項(xiàng)目（09230041010）

  本文關(guān)鍵詞：野菊花中萜類和黃酮類化合物保肝作用研究，由筆耕文化傳播整理發(fā)布。