本文關(guān)鍵詞：霍山石斛多糖對四氯化碳致急性肝損傷小鼠的保護作用，由筆耕文化傳播整理發(fā)布。

霍山石斛多糖對四氯化碳致急性肝損傷小鼠的保護作用

Protective effects of polysaccharides from Dendrobium huoshanense on CCl4-induced acute liver injury in mice

[1] [2] [3] [4] [5] [6]

HUANG Jing,LI Sheng-li, ZHAO Hong-wei, PAN Li-hua, SUN Hao-qiao, LUO Jian-ping（1.Hospital of Hefei University of Technology, Hefei 230009,China;2. School of Biotechnology and Foo

[1]合肥工業(yè)大學(xué)校醫(yī)院,安徽合肥230009; [2]合肥工業(yè)大學(xué)生物與食品工程學(xué)院,安徽合肥230009

文章摘要：目的：研究霍山石斛多糖對四氯化碳敏急性肝損傷小鼠的保護作用。方法：80只小鼠隨機分為8組，分別為正常對照紕、模型對照紺、有旋糖酐對照組、淀粉對照紕、多糖全水解產(chǎn)物對照組、霍山石斛多糖高、中、低（200，100，50mg·kg-1）劑量組，每組10只，各給藥組預(yù)防給藥15d，第15天給藥結(jié)束后灌胃50％（用橄欖油稀釋）的CCl4（1．5mL·kg-1BW），建譏急性肝損傷模型，連續(xù)給藥2d，每天1次。測定小鼠血清ALT和AST及肝勻漿中SOD，，MDA含量，免疫組化法觀察肝細胞中TNF-α表達水平，HE染色觀察肝臟病理組織學(xué)變化。結(jié)果：霍山百斛多糖各刺量組均能降低CCl4致小鼠急性肝損傷血清ALT，AST的升高，降低肝勻漿中MDA含量，增強SOD的活性，抑制肝細胞中TNF—α表達，減輕CCl4對肝組織的病理損傷。結(jié)論：霍山百斛多糖對CCl4致小鼠急性肝損傷具有一定的保護作用，其保護機制可能與清除自由基，抑制脂質(zhì)過氧化，抑制TNF-α表達有關(guān)。

Abstr：Objective：To study the protective effects of polysaccharides from Dendrobium huoshanense（DHP）against CCl4-induced liver injury in mice.Method：Eighty male Kunming mice were randomly divided into normal control group,model control group, destran control group,starch control group,starch control group,three different dose of DPH groups consisting of highdosage group, middle-dosage group and low-dosage group（200,100,50mg·kg-1）.Each group contained ten mice. The mice were treated with DHP via intragastric administration for 15 days before treatment of 50% CCl4 in olive oil for consecutive two days. Both alanine aminotransferase（ALT） and aspartate aminotransferase（AST） activities in serum and superoxide dismutase （SOD） activities and malondialdehyde （MDA） contents in liver tissues were determined in all groups . Immunohistochemistry was used to detect the expression of TNF-α in hepatic tissue. Hepatic histopathological examination was observed. Result：DHP effectively decreased the activities of ALT and AST in serum and the contents of hepatic MDA, and restored hepatic SOD activities in acute liver injury mice. Liver tissue damage induced by CCl4 was ameliorated in mice with DHP administration through histopathology examination. Furthermore, the expression of TNF-α was greatly decreased in groups treated with polysaceharides. Conclusion：DHP has a significantly hepatoprotective effect on CCll4-induced acute liver injury in mice. Protective effect of DHP on the liver may be related to its function of scavenging free radicals and inhibiting lipid peroxidation and TNF-α expression.

文章關(guān)鍵詞：

Keyword：:Dendrobium huoshanense polysaccharide CCl4 acute liver injury

課題項目：國家自然科學(xué)基金項目（20872024）;合肥工業(yè)大學(xué)大學(xué)生創(chuàng)新實驗計劃項目（2011CXCY385）

  本文關(guān)鍵詞：霍山石斛多糖對四氯化碳致急性肝損傷小鼠的保護作用，由筆耕文化傳播整理發(fā)布。