【摘要】：目的探討香延止痛方對寒凝血瘀證原發(fā)性痛經(jīng)大鼠的治療效果及其作用機(jī)制。方法選擇大鼠50只,隨機(jī)分為空白組、模型組、西藥組及中藥低、高劑量組,模型組、西藥組及中藥低、高劑量組建立寒凝血瘀證原發(fā)性痛經(jīng)模型,造模第7天,空白組和模型組予蒸餾水2 mL/只灌胃,西藥組予布洛芬按每日0.002 g灌胃2 mL/只,中藥低劑量組給予香延止痛方水煎劑2 mL/只(含生藥2.38 g),中藥高劑量組給予香延止痛方水煎劑2 mL/只(含生藥11.9 g)。各組均連續(xù)灌胃6天。末次灌胃30 min,除空白組外,其余各組腹腔注射縮宮素,觀察大鼠扭體潛伏時間及30 min內(nèi)扭體反應(yīng)次數(shù)。采用ELISA法檢測各組子宮組織上清液INF-γ、IL-4水平,RT-PCR法檢測子宮組織上清液INF-γ、IL-4 mRNA表達(dá)。結(jié)果中藥低、高劑量組30 min內(nèi)扭體次數(shù)明顯少于模型組(P均0.05),扭體潛伏時間有延長趨勢(P均0.05)。與空白組比較,模型組子宮組織上清液IFN-γ水平降低、IL-4水平升高,IFN-γ/IL-4降低,TNF-αmRNA表達(dá)降低,IL-4 mRNA表達(dá)升高(P均0.05);與模型組比較,中藥低、高劑量組IFN-γ含量升高,IL-4含量降低,IFN-γ/IL-4升高,TNF-αmRNA表達(dá)升高,IL-4 mRNA表達(dá)降低(P均0.05);但中藥低、高劑量組比較差異無統(tǒng)計(jì)學(xué)意義(P均0.05)。結(jié)論寒凝血瘀型原發(fā)性痛經(jīng)大鼠存在Th1/Th2平衡漂移,香延止痛方可能通過調(diào)節(jié)Th1/Th2的平衡狀態(tài)緩解痛經(jīng)癥狀。
[Abstract]:Objective to investigate the therapeutic effect and mechanism of Xiangyan Zhitong recipe on primary dysmenorrhea rats with cold coagulation and blood stasis syndrome. Methods Fifty rats were randomly divided into blank group, model group, western medicine group and low traditional Chinese medicine group. The model of primary dysmenorrhea with cold coagulation and blood stasis syndrome was established in the high dose group. On the 7th day, the blank group and the model group were given distilled water 2 mL/ only intragastrically, and the western medicine group was given ibuprofen 0.002 g per day for 2 mL/. The low dose group of traditional Chinese medicine was given 2 mL/ decoction of Xiangyan Zhitong recipe (containing 2.38 g), of traditional Chinese medicine). The high dose group of traditional Chinese medicine was given 2 mL/ decoction of Xiangyan Zhitong recipe (including 11.9 g). Of crude drug). Each group was given intragastrically for 6 days. Except the blank group, oxytocin was injected intraperitoneally for the last time for 30 min,. The latent time of writhing and the number of writhing reaction in 30 min were observed. The levels of INF- 緯 and IL-4 in the culture medium of each group were detected by ELISA, and the expression of INF- 緯 and IL-4 mRNA in the culture medium of uterine tissue was detected by RT-PCR. Results the number of writhing within 30 min in the high dose group was significantly lower than that in the model group (P 0.05), and the latent time of writhing was prolonged (all P 0.05). Compared with the blank group, the level of IFN- 緯, the level of IL-4, the ratio of IFN- to IL-4, the expression of TNF- 偽 mRNA and the expression of IL-4 mRNA in the uterine tissue of the model group were decreased, and the contents of IFN- 緯, IL-4, IFN- 緯 / IL-4, TNF- 偽 mRNA and IL-4 mRNA in the model group were lower than those in the model group (P < 0.05), while those in the high dose group were significantly higher than those in the model group (P < 0.05), and the expression of TNF- 偽 mRNA and IL-4 mRNA in the model group were significantly higher than those in the model group (P < 0.05). However, there was no significant difference between the high dose group and the low dose group (P 0.05). Conclusion there is Th1/Th2 equilibrium drift in rats with primary dysmenorrhea of cold coagulation and stasis type. Xiangyan Zhitong recipe may relieve dysmenorrhea symptoms by regulating the balance of Th1/Th2.
【作者單位】： 山東中醫(yī)藥大學(xué)第一臨床醫(yī)學(xué)院;山東中醫(yī)藥大學(xué)附屬醫(yī)院;煙臺市中醫(yī)醫(yī)院;山東省醫(yī)學(xué)科學(xué)院基礎(chǔ)醫(yī)學(xué)研究所;
【基金】：山東省重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(2015GSF119010)
【分類號】：R271.113

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