【摘要】：目的:觀察不同刺激量艾灸關(guān)元穴對寒凝證類痛經(jīng)大鼠扭體反應(yīng)及脊髓內(nèi)μ受體表達的影響,探討不同灸量的鎮(zhèn)痛效應(yīng)及其部分中樞機制。方法:按隨機數(shù)字表法將60只雌性SD大鼠,隨機分為鹽水組、寒凝證類痛經(jīng)模型組、A組(艾灸1壯)、B組(艾灸3壯),每組15只。除鹽水組外,其它各組采用全身冷凍法結(jié)合苯甲酸雌二醇注射液加縮宮素注射法制備寒凝證類痛經(jīng)大鼠模型,A組和B組以不同灸量艾灸關(guān)元穴。觀察大鼠扭體反應(yīng),并以熒光定量PCR(RT-PCR)法測定脊髓內(nèi)μ受體mRNA的表達水平。結(jié)果:(1)扭體反應(yīng):與鹽水組比較,模型組、B組扭體潛伏期縮短、扭體次數(shù)、扭體評分明顯增高(P0.05)。與模型組比較,A組、B組扭體次數(shù)、扭體評分明顯減少(P0.05)。(2)脊髓μ受體mRNA的表達:與鹽水組比較,A組與B組的表達均增強(P0.01),與模型組相比,A組與B組的表達增強(P0.01)結(jié)論:(1)不同艾灸量施予關(guān)元穴對寒凝證類痛經(jīng)大鼠模型均有鎮(zhèn)痛作用。(2)艾灸產(chǎn)生鎮(zhèn)痛效應(yīng)的部分機制可能通過增強寒凝證類痛經(jīng)模型大鼠脊髓內(nèi)阿片受體的表達產(chǎn)生鎮(zhèn)痛效應(yīng)。
[Abstract]:Aim: to observe the effects of moxibustion at Guanyuan point on writhing reaction and expression of 渭 receptor in spinal cord of rats with dysmenorrhea with cold coagulation syndrome, and to explore the analgesic effect of different amounts of moxibustion and some central mechanisms. Methods: according to the method of random digital table, 60 female SD rats were randomly divided into saline group, cold coagulation syndrome dysmenorrhea model group and group A (moxibustion 1 strong), B group (moxibustion 3 strong) with 15 rats in each group. Except saline group, the rat model of cold coagulation syndrome dysmenorrhea was established by whole body freezing combined with estridiol benzoate injection and oxytocin injection. Group A and group B were treated with different amounts of moxibustion at Guanyuan point. The writhing reaction of rats was observed, and the expression of 渭 receptor mRNA in spinal cord was measured by fluorescence quantitative PCR (RT-PCR). Results: (1) writhing reaction: compared with saline group, the latency of writhing was shortened, the number of writhing and the score of writhing in group B were significantly higher than those in saline group (P 0.05). Compared with the model group, the number of writhing and the score of writhing in group A and group B were significantly lower than those in group B (P 0.05). (2). The expression of mRNA in spinal cord 渭 receptor in group A and group B was significantly higher than that in group B (P 0.01), and that in group A and group B was significantly higher than that in group B (P 0.01). The expression of group A and group B was increased (P 0.01) conclusion: (1) different doses of moxibustion at Guanyuan acupoint have analgesic effects on the rat model of dysmenorrhea with cold coagulation syndrome. (2) part of the mechanism of analgesic effect of moxibustion may have analgesic effect by increasing the expression of opioid receptor in spinal cord of rats with cold coagulation syndrome dysmenorrhea.
【作者單位】： 北京中醫(yī)藥大學(xué)針灸推拿學(xué)院;威海市中醫(yī)院;國家中醫(yī)藥管理局針灸生物學(xué)三級實驗室;四川省婦幼保健院;北京市蒲黃榆社區(qū)衛(wèi)生服務(wù)中心;國家中醫(yī)藥管理局針灸特色療法評價重點研究室;
【基金】：國家重點基礎(chǔ)研究發(fā)展計劃(973計劃)項目(2012CB518506) 國家中醫(yī)藥管理局針灸特色療法評價重點研究室項目(200905)
【分類號】：R245

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