藏藥喜馬拉雅紫茉莉及陸額的質(zhì)量評價初探
發(fā)布時間：2019-06-18 18:24
【摘要】：本研究通過現(xiàn)代技術(shù)手段對藏藥材喜馬拉雅紫茉莉及陸額進行初步的質(zhì)量評價研究,為兩種藥材合理利用及進一步的開發(fā)提供理論依據(jù)。具體包括以下幾個方面:1.HPLC法測定不同海拔、不同直徑藏藥喜馬拉雅紫茉莉根中葫蘆巴堿的含量方法:NH2色譜柱,流動相乙腈-水(87:13),檢測波長264 nm。結(jié)果:線性范圍1.0109~101.9μg/mL(r=0.9997)。平均回收率95.7%(n=9,RSD=1.12%)。結(jié)果:低海拔(2900 m)藥材中葫蘆巴堿含量171.5 mg/kg,高海拔3630 m以上7組藥材平均含量267.3 mg/kg;某地藥材四種不同直徑(1.5~7.5 cm),葫蘆巴堿含量依次為309.2、319.8、173.8、197.9 mg/kg。另外,某地直徑3.5~4 cm和5.5~7 cm的藥材葫蘆巴堿含量相當,分別為296.11、305.13mg/kg。結(jié)論:推測喜馬拉雅紫茉莉藥材中葫蘆巴堿的含量與海拔、藥材生長年限有關(guān):海拔較高的藥材含量較高。藥材生長初期,葫蘆巴堿含量有上升趨勢,到一定年限后,葫蘆巴堿含量顯著下降。2.喜馬拉雅紫茉莉紅外指紋圖譜研究方法:制備9批藥材的溴化鉀壓片,通過紅外光譜儀檢測并采集譜圖,對比9批藥材的紅外光譜,應用紅外分析軟件(IR solution 1.3)計算相似值,并對相似度值進行聚類分析。結(jié)果:中華紫茉莉與喜馬拉雅紫茉莉的紅外光譜在667.3cm-1處吸收峰有明顯區(qū)別,能準確地區(qū)分二者;相似度結(jié)果顯示喜馬拉雅紫茉莉樣品間相似度均大于0.92,而與中華紫茉莉相似度均不超過0.85;對相似度值進行聚類分析結(jié)果提示喜馬拉雅紫茉莉藥材直徑越相近,其紅外光譜圖更相似。結(jié)論:紅外指紋圖譜可作為鑒別喜馬拉雅紫茉莉與中華紫茉莉的手段。3.HPLC法測定藏藥材陸額中南天竹堿的含量色譜方法:C18色譜柱,流動相:ACN(A),20%ACN+0.1%(v/v)磷酸+0.167%(m/v)SDS(B);梯度洗脫(0~20 min,26%A:20~50min,26%→30%A),檢測波長220 nm。結(jié)果:線性范圍1.200~0.0096 mg/mL(r=0.9995)平均回收率105.8%。本實驗對流動相中SDS加入量進行考察。結(jié)論:該方法準確、穩(wěn)定,可用于檢測陸額中南天竹堿的含量。4.陸額的化學成分研究方法:采用系統(tǒng)分離法對陸額的正丁醇部位進行分離,通過波普數(shù)據(jù)鑒定結(jié)構(gòu)。結(jié)果:從陸額的正丁醇部位中分離鑒定了4個成分:6,7亞甲二氧基-2-甲基-1,2,3,4四氫異喹啉(6,7-Methylenedioxy-2-methyl-1,2,3,4-tetrahydroisoquinoline),前荷葉堿N-氧化物(pronuciferine N-oxide),Papraine,原阿片堿(Protopine)。首次為該植物中分離得到。
[Abstract]:In this study, the preliminary quality evaluation of the Himalayan purple jasmine and the land amount of the Tibetan medicinal materials was carried out by means of modern technical means, and the theoretical basis for the rational utilization and further development of the two kinds of medicinal materials was provided. The method comprises the following aspects:1. the method for determining the content of the cucurbita in the Himalayan purple jasmine root with different altitude and different diameters is determined by the HPLC method, the NH2 chromatographic column, the mobile phase acetonitrile-water (87:13) and the detection wavelength of 264nm are detected. Results: The linear range was 1.0109 ~ 101.9 & mu; g/ mL (r = 0.9997). The average recovery was 95.7% (n = 9, RSD = 1.12%). Results: The average content of fenugreek base in the low altitude (2900m) medicinal materials was 171.5 mg/ kg, the average content was 267.3 mg/ kg in 7 groups with high altitude of 3630 m, and the content of the four different diameters (1.5 to 7.5 cm) of the medicinal materials was 309.2, 319.8, 173.8 and 197.9 mg/ kg. In addition, the content of cucurbitacin in the medicinal materials with a diameter of 3.5 to 4 cm and 5.5 to 7 cm was comparable, which was 296.11 and 305.13 mg/ kg, respectively. Conclusion: The content of fenugreek base in the Himalayan purple jasmine is related to the altitude and the growth period of the medicinal materials: the higher the content of the medicinal materials with higher elevation. In that early stage of the growth of the medicinal material, the content of the cucurbitacin was increase, and after a certain period of time, the content of the fenugreek base decreased significantly. The research method of the infrared fingerprint of the Himalayan purple jasmine is as follows:9 batches of potassium bromide tablets are prepared, and the spectrum images are detected and collected by the infrared spectrometer, the infrared spectrum of the nine batches of medicinal materials is compared, the similar values are calculated by using the infrared analysis software (IR solution 1.3), and the similarity values are clustered. The results show that the infrared spectra of the Chinese violet jasmine and the Himalayan purple jasmine are obviously different from the absorption peak at 667.3 cm-1, and can be divided into two regions; the similarity results show that the similarity between the samples of the Himalayan purple jasmine samples is more than 0.92, and the similarity with the Chinese violet jasmine is not more than 0.85; The results of cluster analysis on the similarity value indicate that the diameter of the Himalayan purple jasmine is similar, and the infrared spectrum is more similar. Conclusion: The infrared fingerprint can be used as a means of identifying the Himalayan purple jasmine and the Chinese violet jasmine.3. The method of HPLC method for the determination of the content of the nanophanine in Tibetan medicinal materials: C18 column, mobile phase: ACN (A),20% ACN + 0.1% (v/ v) phosphoric acid + 0.167% (m/ v) SDS (B), gradient elution (0-20 min,26% A:20-50 min,26% and 30% A), The detection wavelength is 220 nm. Results: The average recovery of linear range of 1.200 ~ 0.0096mg/ mL (r = 0.9995) was 105.8%. The amount of SDS in the mobile phase was investigated in this experiment. Conclusion: The method is accurate and stable, and can be used to detect the content of the bamboo base in the land. The chemical composition of the land was studied by using the system separation method to separate the n-butanol part of the land, and the structure was identified by the Pop data. Results: Four components were identified from the n-butanol fraction of the land,6,7-methylenedioxy-2-methyl-1,2,3,4-dioxy-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2 For the first time in the plant.
【學位授予單位】：西藏大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R29
[Abstract]:In this study, the preliminary quality evaluation of the Himalayan purple jasmine and the land amount of the Tibetan medicinal materials was carried out by means of modern technical means, and the theoretical basis for the rational utilization and further development of the two kinds of medicinal materials was provided. The method comprises the following aspects:1. the method for determining the content of the cucurbita in the Himalayan purple jasmine root with different altitude and different diameters is determined by the HPLC method, the NH2 chromatographic column, the mobile phase acetonitrile-water (87:13) and the detection wavelength of 264nm are detected. Results: The linear range was 1.0109 ~ 101.9 & mu; g/ mL (r = 0.9997). The average recovery was 95.7% (n = 9, RSD = 1.12%). Results: The average content of fenugreek base in the low altitude (2900m) medicinal materials was 171.5 mg/ kg, the average content was 267.3 mg/ kg in 7 groups with high altitude of 3630 m, and the content of the four different diameters (1.5 to 7.5 cm) of the medicinal materials was 309.2, 319.8, 173.8 and 197.9 mg/ kg. In addition, the content of cucurbitacin in the medicinal materials with a diameter of 3.5 to 4 cm and 5.5 to 7 cm was comparable, which was 296.11 and 305.13 mg/ kg, respectively. Conclusion: The content of fenugreek base in the Himalayan purple jasmine is related to the altitude and the growth period of the medicinal materials: the higher the content of the medicinal materials with higher elevation. In that early stage of the growth of the medicinal material, the content of the cucurbitacin was increase, and after a certain period of time, the content of the fenugreek base decreased significantly. The research method of the infrared fingerprint of the Himalayan purple jasmine is as follows:9 batches of potassium bromide tablets are prepared, and the spectrum images are detected and collected by the infrared spectrometer, the infrared spectrum of the nine batches of medicinal materials is compared, the similar values are calculated by using the infrared analysis software (IR solution 1.3), and the similarity values are clustered. The results show that the infrared spectra of the Chinese violet jasmine and the Himalayan purple jasmine are obviously different from the absorption peak at 667.3 cm-1, and can be divided into two regions; the similarity results show that the similarity between the samples of the Himalayan purple jasmine samples is more than 0.92, and the similarity with the Chinese violet jasmine is not more than 0.85; The results of cluster analysis on the similarity value indicate that the diameter of the Himalayan purple jasmine is similar, and the infrared spectrum is more similar. Conclusion: The infrared fingerprint can be used as a means of identifying the Himalayan purple jasmine and the Chinese violet jasmine.3. The method of HPLC method for the determination of the content of the nanophanine in Tibetan medicinal materials: C18 column, mobile phase: ACN (A),20% ACN + 0.1% (v/ v) phosphoric acid + 0.167% (m/ v) SDS (B), gradient elution (0-20 min,26% A:20-50 min,26% and 30% A), The detection wavelength is 220 nm. Results: The average recovery of linear range of 1.200 ~ 0.0096mg/ mL (r = 0.9995) was 105.8%. The amount of SDS in the mobile phase was investigated in this experiment. Conclusion: The method is accurate and stable, and can be used to detect the content of the bamboo base in the land. The chemical composition of the land was studied by using the system separation method to separate the n-butanol part of the land, and the structure was identified by the Pop data. Results: Four components were identified from the n-butanol fraction of the land,6,7-methylenedioxy-2-methyl-1,2,3,4-dioxy-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2 For the first time in the plant.
【學位授予單位】：西藏大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R29
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