【摘要】：目的：觀察不同劑量養(yǎng)陰潤腸方含藥血清對離體培養(yǎng)的小鼠結腸Cajal間質(zhì)細胞(Interstitial Cells of Cajal ICC)凋亡和周期調(diào)控的影響,為養(yǎng)陰潤腸方治療慢傳輸型便秘(slow transit constipation STC)提供理論依據(jù)。方法：將20只BALB/c小鼠隨機分為四組,原代分離和培養(yǎng)每組小鼠結腸組織,通過細胞流式分選技術分選出成熟的ICC,用提前制備好的正常大鼠血清及高、中、低劑量養(yǎng)陰潤腸方含藥血清對ICC進行分組干預,干預2d后觀察ICC數(shù)量及其形態(tài)學改變,并運用流式檢測技術評定ICC凋亡機制和細胞周期調(diào)控作用。結果：各組培養(yǎng)所得細胞經(jīng)相應的干預后,鏡下觀察后統(tǒng)計高、中、低及空白劑量組細胞平均計數(shù)分別為(13.94±2.64；10.17±1.33；8.17±1.47；6.33±1.63),高劑量和中劑量組細胞數(shù)量較空白組表達升高(P0.01),低劑量組與—表達無明顯差異(P0.05),且低劑量組與高劑量組存在統(tǒng)計學差異(P0.01),中劑量組與高劑量組和低劑量組之間無統(tǒng)計學差異(P0.05)。在細胞凋亡作用上,早凋亡和晚凋亡之總和為總凋亡率,空白組、養(yǎng)陰潤腸方含藥血清高、中、低劑量組早期和晚期凋亡和為16.9%、9.5%、12.4%、12.6%,總凋亡率：空白組低劑量組中劑量組高劑量組。養(yǎng)陰潤腸方血清能有效減少cajal細胞凋亡。細胞周期調(diào)控作用中,空白組、養(yǎng)陰潤腸方含藥血清高、中、低劑量組S期和G2期細胞總和所占比分別為36.52%、41.02%、43.86%、82.40%,含藥血清組內(nèi)正處于復制活動期的ICC數(shù)量所占比例高劑量組中劑量組低劑量組空白劑量組。結論：養(yǎng)陰潤腸方能促進ICC數(shù)量的增長,并能有效地減少ICC凋亡數(shù)量和提高細胞分裂能力及活性,其通便機制可能為通過促進ICC的數(shù)量生長及減少其凋亡而達到促進腸動力,為治療STC尋求一條新的途徑。
[Abstract]:Objective: to observe the effect of different doses of Yangyin Runchang recipe containing serum on (Interstitial Cells of Cajal ICC) apoptosis and cycle regulation of mouse colon Cajal Leydig cells in vitro. It provides a theoretical basis for Yangyin Runchang recipe in the treatment of slow transit constipation (slow transit constipation STC). Methods: 20 BALB/c mice were randomly divided into four groups. The colon tissue of each group was isolated and cultured. Mature ICC, was separated by flow cytometry and normal rat serum was prepared in advance. The serum containing low dose Yangyin Runchang recipe was divided into groups to intervene ICC. After 2 days of intervention, the number and morphological changes of ICC were observed, and the apoptosis mechanism and cell cycle regulation of ICC were evaluated by flow cytometry. Results: after the corresponding intervention, the average cell count of the cultured cells in the middle, low and blank dose groups was (13.94 鹵2.64) 鈮，

本文編號：2484912