發(fā)布時間：2016-12-09 21:15

  本文關(guān)鍵詞：Kinesin，由筆耕文化傳播整理發(fā)布。

Domain

Kinesin motor domain (IPR001752)

Short name: Kinesin_motor_dom

Domain relationships

None.

Description

Kinesin [PMID: 8542443, PMID: 2142876, PMID: 14732151] is a microtubule-associated force-producing protein that may play a role in organelle transport. The kinesin motor activity is directed toward the microtubule's plus end. Kinesin is an oligomeric complex composed of two heavy chains and two light chains. The maintenance of the quaternary structure does not require interchain disulphide bonds.

The heavy chain is composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it is known to hydrolyse ATP, to bind and move on microtubules), a central alpha-helical coiled coil domain that mediates the heavy chain dimerisation; and a small globular C-terminal domain which interacts with other proteins (such as the Kinesin light chains), vesicles and membranous organelles.

The Kinesin motor domain comprises five motifs, namely N1 (P-loop), N2 (Switch I), N3 (Switch II), N4 and L2 (KVD finger) [PMID: 20587735]. It has a mixed eight stranded beta-sheet core with flanking solvent exposed alpha-helices and a small three-stranded antiparallel beta-sheet in the N-terminal region [PMID: 15236970].

A number of proteins have been recently found that contain a domain similar to that of the Kinesin 'motor' domain [PMID: 8542443, PMID: 1832505]:

The Kinesin motor domain is located in the N-terminal part of most of the above proteins, with the exception of KAR3, klpA, and ncd where it is located in the C-terminal section.

The Kinesin motor domain contains about 330 amino acids. An ATP-binding motif of type A is found near position 80 to 90, the C-terminal half of the domain is involved in microtubule-binding.

Bloom GS, Endow SA.
Motor proteins 1: Kinesins.
null 2 1105-71 1995

Vallee RB, Shpetner HS.
Motor proteins of cytoplasmic microtubules.
Annu. Rev. Biochem. 59 909-32 1990

Brady ST.
A Kinesin medley: biochemical and functional heterogeneity.
Trends Cell Biol. 5 159-64 1995

Kitagawa K, Kurinami S, Oki K, Abe Y, Ando T, Kono I, Yano M, Kitano H, Iwasaki Y.
A novel Kinesin 13 protein regulating rice seed length.
Plant Cell Physiol. 51 1315-29 2010

Garcia-Saez I, Yen T, Wade RH, Kozielski F.
Crystal structure of the motor domain of the human kinetochore protein CENP-E.
J. Mol. Biol. 340 1107-16 2004

Bloom GS, Endow SA.
Motor proteins 1: Kinesins.
null 2 1105-71 1995

Endow SA.
The emerging Kinesin family of microtubule motor proteins.
Trends Biochem. Sci. 16 221-5 1991

Endow SA.
The emerging Kinesin family of microtubule motor proteins.
Trends Biochem. Sci. 16 221-5 1991

GO terms

Biological Process

GO:0007018 microtubule-based movement

Molecular Function

GO:0005524 ATP binding
GO:0008017 microtubule binding
GO:0003777 microtubule motor activity

Cellular Component

No terms assigned in this category.

  本文關(guān)鍵詞：Kinesin，，由筆耕文化傳播整理發(fā)布。

本文編號：208961