本文關鍵詞：基于高效液相色譜-飛行時間質譜技術的高血壓病肝陽上亢證尿液代謝組學研究，，由筆耕文化傳播整理發(fā)布。

蔣海強,李運倫,解君.基于高效液相色譜-飛行時間質譜技術的高血壓病肝陽上亢證尿液代謝組學研究[J].,2012,32(3):333-337

基于高效液相色譜-飛行時間質譜技術的高血壓病肝陽上亢證尿液代謝組學研究

Urine Metabonomic Study on Hypertension Patients of Ascendant Hyperactivity of Gan Yang Syndrome by High Performance Liquid Chromatography Coupled with Time of Flight Mass Spectrometry

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DOI：

中文關鍵詞:  代謝組學  高效液相色譜-飛行時間質譜技術  模式識別  高血壓病肝陽上亢證

英文關鍵詞:metabolomics  high performance liquid chromatography coupled with time of flight mass spectrometry  pattern recognition  hypertension of ascendant hyperactivity of Gan yang syndrome

基金項目:國家自然科學基金資助項目(No.30772865);教育部新世紀優(yōu)秀人才支持計劃資助項目(No.NCET-07-0522);山東省優(yōu)秀中青年科學家獎勵基金資助項目(No.2008BS03029);山東省2009—2010中醫(yī)藥科技發(fā)展計劃資助項目(No.2009-018);山東省“泰山學者”建設工程專項經費資助項目

作者單位

蔣海強 山東中醫(yī)藥大學實驗中心 

李運倫 山東中醫(yī)藥大學附屬醫(yī)院高血壓中醫(yī)臨床研究基地 

解君 山東中醫(yī)藥大學實驗中心 

摘要點擊次數: 456

全文下載次數: 5

中文摘要:

      目的研究高血壓病肝陽上亢證患者的尿液代謝物變化,尋找其特征代謝物,探索高血壓病肝陽上亢證的證候本質。方法選擇高血壓病肝陽上亢證患者10例為患者組,另設12名健康體檢者為正常組,采用高效液相色譜-飛行時間質譜技術(HPLC-TOFMS)采集兩組尿液代謝物譜,利用SIMCA-P軟件進行主成分分析(principal component analysis,PCA)及偏最小二乘判別分析(partial least-square discriminant analysis,PLS-DA),尋找并鑒定尿液內差異代謝物,做出相關代謝途徑的可能解釋。結果 PCA方法處理患者組和正常組的尿液代謝物譜數據顯示,兩組HPLC-TOFMS譜數據可以明顯分開。與正常組比較,患者組機體相關代謝發(fā)生顯著變化。經PLS-DA確定與高血壓病肝陽上亢證有關的代謝物,通過查詢KEGG數據庫共確定了15個化合物的結構及代謝途徑,主要包括氨基酸、游離脂肪酸、神經鞘氨醇等。結論采用HPLC-TOFMS結合模式識別的代謝組學方法對高血壓病肝陽上亢證患者尿液代謝物進行研究,從微觀領域尋找小分子代謝標記物,有利于深入發(fā)現中醫(yī)證候的生物學本質。

英文摘要:

      Objective To study the changes of urine metabolites in hypertension patients of ascendant hyperactivity of Gan yang syndrome(AHGYS) ,and to explore its essence in hypertension patients.Methods Ten typical hypertension patients of AHGYS were recruited as the patient group,and the other twelve healthy volunteers were recruited as the normal group.The metabolite profiling in the urine were collected using by high performance liquid chromatography coupled with time of flight mass spectrometry(HPLC-TOFMS) .The principal component analysis(PCA) and partial least-square discriminant analysis(PLS-DA) were analyzed using SIMCA-P Software.The differential metabolites in the urine were found out and identified.The possible relevant metabolic pathways were explained.Results The data from the analysis by PCA in the urine samples of the patient group and the normal group showed,two sets of data could be obviously classified in the score plot.Compared with the normal group,significant changes happened to the body metabolism in the patient group.The metabolites relevant to hypertension patients of AHGYS were determined using the PLS-DA.Fifteen compounds of the structure and metabolic pathways had been confirmed through inquiring KEGG Database,mainly including amino acids,free fatty acids,sphingosine,and so on.Conclusions The hypertension patients of AHGYS were studied using HPLC-TOFMS combined with pattern recognition,thus finding out small molecular metabolic markers from the microscopic field,which was advantageous in probing the biological nature of Chinese medicine syndromes.

  本文關鍵詞：基于高效液相色譜-飛行時間質譜技術的高血壓病肝陽上亢證尿液代謝組學研究，由筆耕文化傳播整理發(fā)布。