雷公藤甲素對大鼠坐骨神經(jīng)冷保存后細(xì)胞活性及異體移植后神經(jīng)再生的影響
發(fā)布時間：2022-02-04 18:37
目的探討雷公藤甲素（T10）對冷保存大鼠坐骨神經(jīng)生物活性和異體移植后神經(jīng)再生的影響。方法取對數(shù)生長期施萬細(xì)胞（SCs）,CCK-8檢測10-66 mol/L、10-77 mol/L、10-88 mol/L、10-99 mol/L T10溶液對SCs增殖的影響。取Sprague-Dawley雌性大鼠雙側(cè)坐骨神經(jīng)15 mm,分別在含0 mol/L、10-66 mol/L、10-7mol/L、10-88 mol/L、10-99 mol/L T10的DMEM液中,4℃或37℃培養(yǎng)24 h（n=6）,Western blotting檢測神經(jīng)生長因子（NGF）、神經(jīng)膠質(zhì)細(xì)胞源性神經(jīng)營養(yǎng)因子（GDNF）、腦源性神經(jīng)營養(yǎng)因子（BDNF）表達(dá)。另取大鼠坐骨神經(jīng)15 mm,隨機分成新鮮神經(jīng)組（A組,n=30）、DMEM保存組（B組,n=30）、T10保存組（C組,n=30）、T10預(yù)處理后DMEM保存組（D組,n=30）、T10預(yù)處...
【文章來源】：重慶醫(yī)科大學(xué)重慶市
【文章頁數(shù)】：52 頁
【學(xué)位級別】：碩士
【部分圖文】：
雷公藤甲素（T10）對雪旺細(xì)胞的細(xì)胞毒性Fig1.CytotoxicityofT10onSCs.Comparisonbetweenthe10-6MT10-treatedand
圖 2: 雷公藤甲素預(yù)處理誘導(dǎo)培養(yǎng)坐骨神經(jīng) NGF、BDNF、GDNF 蛋白表達(dá)Fig 2. Levels of NGF, BDNF, and GDNF in cultured sciatic nerves pretreated withT10. Note :1) Significantly increased levels of NGF, BDNF, and GDNF were detectedfor all of the 37°C pretreatment groups compared with those for all of the 4°Cpretreatment groups (P<0.001, P<0.001, and P<0.001, respectively, Fig. 2A-C). 2) Forthe 4°C pretreatment groups, the 10-8M T10-treated group had significantly increasedNGF levels and increased levels of BDNFcompared with the groups receiving otherconcentrations of T10 (*P<0.001and *P=0.03, Fig. 2A and 2C).But no obviousdifferences were observed for GDNF expression levels among the groups (*P=0.147,Fig. 2B). 3) For the 37°C pretreatment groups, the 10-8M T10-treated groups hadsignificantly increased levels of NGF、GDNF and BDNF expression compared withthe other T10-treated groups ( **P<0.001, P<0.001, and P<0.001, respectively, Fig.2A-C).
D 組 E 組圖 3：激光共聚焦顯微鏡觀察冷保存神經(jīng)細(xì)胞活性Fig 3. Observation of cryopreserved nerve cell activity using laser confocalmicroscopy.
【參考文獻(xiàn)】：
期刊論文
[1]Tissue engineering for the repair of peripheral nerve injury[J]. Pei-Xun Zhang,Na Han,Yu-Hui Kou,Qing-Tang Zhu,Xiao-Lin Liu,Da-Ping Quan,Jian-Guo Chen,Bao-Guo Jiang. Neural Regeneration Research. 2019(01)
[2]Analysis of transcriptome sequencing of sciatic nerves in Sprague-Dawley rats of different ages[J]. Jiang-Hui Liu,Qing Tang,Xiang-Xia Liu,Jian Qi,Rui-Xi Zeng,Zhao-Wei Zhu,Bo He,Yang-Bin Xu. Neural Regeneration Research. 2018(12)
[3]Decellularized sciatic nerve matrix as a biodegradable conduit for peripheral nerve regeneration[J]. Jongbae Choi,Jun Ho Kim,Ji Wook Jang,Hyun Jung Kim,Sung Hoon Choi,Sung Won Kwon. Neural Regeneration Research. 2018(10)
[4]冷誘導(dǎo)RNA結(jié)合蛋白促進(jìn)冷凍保存大鼠坐骨神經(jīng)異體移植后神經(jīng)再生的作用[J]. 李子健,黃英如,曾歡歡,汪一,張松. 中國康復(fù)理論與實踐. 2018(04)
[5]Stimulating effect of thyroid hormones in peripheral nerve regeneration:research history and future direction toward clinical therapy[J]. I.Barakat-Walter,R.Kraftsik. Neural Regeneration Research. 2018(04)
[6]Autologous transplantation with fewer fibers repairs large peripheral nerve defects[J]. Jiu-xu Deng,Dian-yin Zhang,Ming Li,Jian Weng,Yu-hui Kou,Pei-xun Zhang,Na Han,Bo Chen,Xiao-feng Yin,Bao-guo Jiang. Neural Regeneration Research. 2017(12)
[7]中醫(yī)藥促進(jìn)周圍神經(jīng)損傷修復(fù)的研究進(jìn)展[J]. 陳傳奇,陳龍菊. 時珍國醫(yī)國藥. 2016(09)
[8]周圍神經(jīng)損傷后軸突再生微環(huán)境的研究進(jìn)展[J]. 范紅石,王艷,陳國平. 中國康復(fù)理論與實踐. 2015(03)
[9]What’s new in clinical solid organ transplantation by 2013[J]. Maurizio Salvadori,Elisabetta Bertoni. World Journal of Transplantation. 2014(04)
[10]中醫(yī)治療周圍神經(jīng)損傷探討[J]. 李玲. 中醫(yī)研究. 2014(08)
本文編號：3613732
【文章來源】：重慶醫(yī)科大學(xué)重慶市
【文章頁數(shù)】：52 頁
【學(xué)位級別】：碩士
【部分圖文】：
雷公藤甲素（T10）對雪旺細(xì)胞的細(xì)胞毒性Fig1.CytotoxicityofT10onSCs.Comparisonbetweenthe10-6MT10-treatedand
圖 2: 雷公藤甲素預(yù)處理誘導(dǎo)培養(yǎng)坐骨神經(jīng) NGF、BDNF、GDNF 蛋白表達(dá)Fig 2. Levels of NGF, BDNF, and GDNF in cultured sciatic nerves pretreated withT10. Note :1) Significantly increased levels of NGF, BDNF, and GDNF were detectedfor all of the 37°C pretreatment groups compared with those for all of the 4°Cpretreatment groups (P<0.001, P<0.001, and P<0.001, respectively, Fig. 2A-C). 2) Forthe 4°C pretreatment groups, the 10-8M T10-treated group had significantly increasedNGF levels and increased levels of BDNFcompared with the groups receiving otherconcentrations of T10 (*P<0.001and *P=0.03, Fig. 2A and 2C).But no obviousdifferences were observed for GDNF expression levels among the groups (*P=0.147,Fig. 2B). 3) For the 37°C pretreatment groups, the 10-8M T10-treated groups hadsignificantly increased levels of NGF、GDNF and BDNF expression compared withthe other T10-treated groups ( **P<0.001, P<0.001, and P<0.001, respectively, Fig.2A-C).
D 組 E 組圖 3：激光共聚焦顯微鏡觀察冷保存神經(jīng)細(xì)胞活性Fig 3. Observation of cryopreserved nerve cell activity using laser confocalmicroscopy.
【參考文獻(xiàn)】：
期刊論文
[1]Tissue engineering for the repair of peripheral nerve injury[J]. Pei-Xun Zhang,Na Han,Yu-Hui Kou,Qing-Tang Zhu,Xiao-Lin Liu,Da-Ping Quan,Jian-Guo Chen,Bao-Guo Jiang. Neural Regeneration Research. 2019(01)
[2]Analysis of transcriptome sequencing of sciatic nerves in Sprague-Dawley rats of different ages[J]. Jiang-Hui Liu,Qing Tang,Xiang-Xia Liu,Jian Qi,Rui-Xi Zeng,Zhao-Wei Zhu,Bo He,Yang-Bin Xu. Neural Regeneration Research. 2018(12)
[3]Decellularized sciatic nerve matrix as a biodegradable conduit for peripheral nerve regeneration[J]. Jongbae Choi,Jun Ho Kim,Ji Wook Jang,Hyun Jung Kim,Sung Hoon Choi,Sung Won Kwon. Neural Regeneration Research. 2018(10)
[4]冷誘導(dǎo)RNA結(jié)合蛋白促進(jìn)冷凍保存大鼠坐骨神經(jīng)異體移植后神經(jīng)再生的作用[J]. 李子健,黃英如,曾歡歡,汪一,張松. 中國康復(fù)理論與實踐. 2018(04)
[5]Stimulating effect of thyroid hormones in peripheral nerve regeneration:research history and future direction toward clinical therapy[J]. I.Barakat-Walter,R.Kraftsik. Neural Regeneration Research. 2018(04)
[6]Autologous transplantation with fewer fibers repairs large peripheral nerve defects[J]. Jiu-xu Deng,Dian-yin Zhang,Ming Li,Jian Weng,Yu-hui Kou,Pei-xun Zhang,Na Han,Bo Chen,Xiao-feng Yin,Bao-guo Jiang. Neural Regeneration Research. 2017(12)
[7]中醫(yī)藥促進(jìn)周圍神經(jīng)損傷修復(fù)的研究進(jìn)展[J]. 陳傳奇,陳龍菊. 時珍國醫(yī)國藥. 2016(09)
[8]周圍神經(jīng)損傷后軸突再生微環(huán)境的研究進(jìn)展[J]. 范紅石,王艷,陳國平. 中國康復(fù)理論與實踐. 2015(03)
[9]What’s new in clinical solid organ transplantation by 2013[J]. Maurizio Salvadori,Elisabetta Bertoni. World Journal of Transplantation. 2014(04)
[10]中醫(yī)治療周圍神經(jīng)損傷探討[J]. 李玲. 中醫(yī)研究. 2014(08)
本文編號：3613732
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