認(rèn)知缺陷是抑郁癥的核心癥狀之一,已成為抑郁癥治療面臨的的一大挑戰(zhàn).花櫚木為我國南方廣泛分布的一種綠色喬木,具有抗抑郁作用.然而,花櫚木對認(rèn)知損害的作用尚未見報(bào)道.本研究旨在探討花櫚木提取物對慢性應(yīng)激引起的認(rèn)知障礙的影響,通過建立小鼠慢性不可預(yù)測輕度應(yīng)激（CUMS）模型,評價(jià)花櫚木改善認(rèn)知功能的作用.認(rèn)知行為采用新物識別和Morris水迷宮測試,免疫組織化學(xué)分析海馬組織中GFAP和Aβ蛋白的水平,Western Blotting分析海馬組織NLRP3、TNF-α和IL-1β蛋白表達(dá).結(jié)果顯示,花櫚木提取物能明顯增加小鼠的物體識別辨別指數(shù),明顯減少定位航行小鼠平臺潛伏期,增加平臺穿越次數(shù).花櫚木提取物能顯著提高海馬組織中5-HT、DA、NE的水平;同時(shí)提高應(yīng)激小鼠海馬CA1區(qū)GFAP水平,降低Aβ沉積,減少海馬區(qū)NLRP3、TNF-α和IL-1β的表達(dá).以上數(shù)據(jù)表明,花櫚木提取物能明顯改善CUMS小鼠學(xué)習(xí)記憶障礙,其作用機(jī)制與上調(diào)GAFP、減少Aβ沉積、緩解炎性有關(guān). 

【文章來源】：生物化學(xué)與生物物理進(jìn)展. 2020,47(08)北大核心SCICSCD

【文章頁數(shù)】：12 頁

【文章目錄】：
1 Materials and methods
    1.1 Plant material and reagents
    1.2 Animals
    1.3 Establishment of chronic unpredictable mild stress model(CUMS)
    1.4 Grouping and administration
    1.5 Behavioral assessment
        1.5.1 Novel object recognition test(NORT)
        1.5.2 Morris water maze test(MWM)
    1.6 Neurotransmitters in hippocampal tissue in CUMS mice
    1.7 Histology
    1.8 Immunohistochemistry
    1.9 Western blot analysis
    1.1 0 Statistical analysis
    1.1 1 The experimental procedure
2 Results
    2.1 OHP alleviated the deficits of recognition memory in CUMS mice
    2.2 OHP ameliorated the deficits of spatial learning and memory in CUMS mice
    2.3 Effects of OHP on neurotransmitters in hippocampal tissue in CUMS mice
    2.4 The histopathology of hippocampus in CUMS mice
    2.5 OHP decreased the expression of GFAP and Aβprotein in the hippocampus of CUMS mice
    2.6 Effects of OHP on the expression of NLRP3,TNF-α，IL-1βin hippocampus of mice
3 Discussion
4 Conclusion


【參考文獻(xiàn)】：
期刊論文
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[2]Celastrol specifically inhibits the activation of NLRP3 inflammasome[J]. Xuesong Sang,Yihui Chen,Wei Chen,Jing Xie,Guangxun Meng,Jin Zhong,Taisheng Li,Ailing Lu.  Science China（Life Sciences）. 2018(03)
[3]花梨木精油通過嗅覺通路改善小鼠抑郁樣行為及其神經(jīng)遞質(zhì)[J]. 陳軍,徐金勇,徐蓉,劉莉茵,任振華,李光武.  江蘇醫(yī)藥. 2012(06)



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