本文選題：射頻電磁場 + 工頻電磁場��； 參考：《浙江大學》2012年碩士論文

【摘要】：隨著電力事業(yè)和無線通訊技術(shù)的快速發(fā)展,環(huán)境中工頻和射頻電磁場的暴露時間、強度和范圍與日俱增,公眾對其可能的健康影響日益關(guān)注。雖然有流行病學研究顯示電磁場與某些腫瘤的發(fā)生相關(guān),但研究結(jié)果不一致,并缺乏實驗室證據(jù)的支持。由于DNA損傷與腫瘤的發(fā)生發(fā)展密切相關(guān),因此,電磁場對DNA的損傷效應(yīng)研究成為了電磁場危險度評估中的熱點問題。然而,目前有關(guān)電磁場對DNA損傷作用的實驗室研究結(jié)果很不一致,究其原因,可能與研究者采用的研究方法、研究模型等不同有關(guān)。我們認為,作為一種環(huán)境物理性“弱”作用因素,必須采用更靈敏的方法和系統(tǒng)的研究策略,才有可能真實地揭示電磁場的效應(yīng)。因此,本學位論文采用DNA雙鏈斷裂的“金標準”——γH2AX焦點形成分析技術(shù),以視覺系統(tǒng)和神經(jīng)系統(tǒng)的代表性細胞——人眼晶狀體上皮細胞(HLEC)、SD大鼠小膠質(zhì)細胞(Microglia)和人腦膠質(zhì)瘤細胞(U251)為研究對象,初步評價了電磁場對視覺系統(tǒng)和神經(jīng)系統(tǒng)的代表性細胞DNA雙鏈斷裂的影響。 將上述細胞分別暴露于4W/kg的1800MHz GSM (Global system for mobile communications,全球移動通訊系統(tǒng))射頻電磁場和2mT的50Hz工頻磁場0.5-24小時,采用yH2AX焦點免疫熒光成像法檢測其yH2AX焦點形成情況。結(jié)果顯示：與假輻照組相比,4W/kg的1800MHz GSM射頻電磁場輻照24小時后,U251細胞yH2AX平均焦點數(shù)降低(p0.05)；不同時間射頻電磁場輻照Microglia與HLEC細胞,未能引起細胞yH2AX平均焦點數(shù)、yH2AX陽性細胞率和yH2AX焦點分段分布率的變化(p0.05)；此外,2mT的50Hz工頻磁場輻照未能引起U251、Microglia和HLEC細胞的yH2AX焦點的變化(p0.05)。 根據(jù)以上研究結(jié)果,我們認為在本實驗條件下：1)電磁場對HLEC和Microglia的DNA雙鏈斷裂沒有影響；2)1800MHz GSM射頻電磁場輻照24小時降低了U251細胞中的yH2AX平均焦點數(shù),射頻電磁場引起該效應(yīng)的作用機制及其和衛(wèi)生學意義有待于進一步研究。
[Abstract]:With the rapid development of power industry and wireless communication technology, the exposure time, intensity and range of power frequency and radio frequency electromagnetic field in the environment are increasing, and the public is paying more and more attention to its possible health effects.Although epidemiological studies have shown that electromagnetic fields are associated with the occurrence of certain tumors, the results are inconsistent and lack laboratory evidence.Because DNA damage is closely related to the occurrence and development of tumor, the study of electromagnetic field damage effect on DNA has become a hot issue in electromagnetic field risk assessment.However, the results of laboratory studies on the effects of electromagnetic fields on DNA damage are very inconsistent. The reasons may be related to the different research methods and models used by researchers.We think that as a kind of environmental physical "weak" action factor, we must adopt more sensitive methods and systematic research strategies in order to truly reveal the effect of electromagnetic field.Therefore, this dissertation adopts the "gold standard"-緯-H2AX focus formation analysis technique of DNA double strand breakage.The typical cells of the visual system and nervous system, human lens epithelial cells (HLECE), microglia of SD rats and human glioma cell U251) were studied.The effects of electromagnetic fields on the DNA double strand breaks in the representative cells of the visual system and nervous system were preliminarily evaluated.The cells were exposed to 1800MHz GSM system for mobile communications (global mobile communication system) of 4W/kg and 50Hz of 2mT for 0.5 to 24 hours, respectively. YH2AX focal immunofluorescence imaging was used to detect the formation of yH2AX focus.The results showed that the mean yH2AX focal point number of U251 cells was decreased by 4W / kg 1800MHz GSM radiofrequency electromagnetic field (RF-EMF) for 24 hours compared with that of false irradiation group, and the mean yH2AX focal point number of U251 cells was decreased by RF-EMF irradiation at different times.The changes of the cell yH2AX mean focal number and the distribution rate of yH2AX focus segment were not induced (p 0.05), and the change of yH2AX focus of U251 microglia and HLEC cells was not induced by 2mT 50Hz power frequency magnetic field irradiation (P 0.05).Based on the above results, we believe that under this experimental condition, there is no effect on the DNA double strand breaks of HLEC and Microglia under the condition of 1: 1) GSM radiofrequency electromagnetic field irradiation at 21800MHz for 24 hours can reduce the average number of yH2AX focal points in U251 cells.The mechanism of the effect caused by RF electromagnetic field and its hygienic significance need to be further studied.
【學位授予單位】：浙江大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R142

【參考文獻】

相關(guān)期刊論文 前2條

1 姜槐,許正平;電磁輻射與人體健康[J];中華勞動衛(wèi)生職業(yè)病雜志;2002年04期

2 許正平,姜槐;電磁場健康風險評估和標準制定勢在必行[J];中華預(yù)防醫(yī)學雜志;2004年01期

，

本文編號：1740007